Pyk2 in dorsal hippocampus plays a selective role in spatial memory and synaptic plasticity

Abstract Pyk2 is a Ca2+-activated non-receptor tyrosine kinase enriched in the forebrain, especially in pyramidal neurons of the hippocampus. Previous reports suggested its role in hippocampal synaptic plasticity and spatial memory but with contradictory findings possibly due to experimental conditi...

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Autores principales:	Vincenzo Mastrolia, Omar al Massadi, Benoit de Pins, Jean-Antoine Girault
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/25fc90fc77e143baa5cc1dd54097531e
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spelling	oai:doaj.org-article:25fc90fc77e143baa5cc1dd54097531e2021-12-02T16:27:50ZPyk2 in dorsal hippocampus plays a selective role in spatial memory and synaptic plasticity10.1038/s41598-021-95813-x2045-2322https://doaj.org/article/25fc90fc77e143baa5cc1dd54097531e2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95813-xhttps://doaj.org/toc/2045-2322Abstract Pyk2 is a Ca2+-activated non-receptor tyrosine kinase enriched in the forebrain, especially in pyramidal neurons of the hippocampus. Previous reports suggested its role in hippocampal synaptic plasticity and spatial memory but with contradictory findings possibly due to experimental conditions. Here we address this issue and show that novel object location, a simple test of spatial memory induced by a single training session, is altered in Pyk2 KO mice and that re-expression of Pyk2 in the dorsal hippocampus corrects this deficit. Bilateral targeted deletion of Pyk2 in dorsal hippocampus CA1 region also alters novel object location. Long term potentiation (LTP) in CA1 is impaired in Pyk2 KO mice using a high frequency stimulation induction protocol but not with a theta burst protocol, explaining differences between previous reports. The same selective LTP alteration is observed in mice with Pyk2 deletion in dorsal hippocampus CA1 region. Thus, our results establish the role of Pyk2 in specific aspects of spatial memory and synaptic plasticity and show the dependence of the phenotype on the type of experiments used to reveal it. In combination with other studies, we provide evidence for a selective role of non-receptor tyrosine kinases in specific aspects of hippocampal neurons synaptic plasticity.Vincenzo MastroliaOmar al MassadiBenoit de PinsJean-Antoine GiraultNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vincenzo Mastrolia Omar al Massadi Benoit de Pins Jean-Antoine Girault Pyk2 in dorsal hippocampus plays a selective role in spatial memory and synaptic plasticity
description	Abstract Pyk2 is a Ca2+-activated non-receptor tyrosine kinase enriched in the forebrain, especially in pyramidal neurons of the hippocampus. Previous reports suggested its role in hippocampal synaptic plasticity and spatial memory but with contradictory findings possibly due to experimental conditions. Here we address this issue and show that novel object location, a simple test of spatial memory induced by a single training session, is altered in Pyk2 KO mice and that re-expression of Pyk2 in the dorsal hippocampus corrects this deficit. Bilateral targeted deletion of Pyk2 in dorsal hippocampus CA1 region also alters novel object location. Long term potentiation (LTP) in CA1 is impaired in Pyk2 KO mice using a high frequency stimulation induction protocol but not with a theta burst protocol, explaining differences between previous reports. The same selective LTP alteration is observed in mice with Pyk2 deletion in dorsal hippocampus CA1 region. Thus, our results establish the role of Pyk2 in specific aspects of spatial memory and synaptic plasticity and show the dependence of the phenotype on the type of experiments used to reveal it. In combination with other studies, we provide evidence for a selective role of non-receptor tyrosine kinases in specific aspects of hippocampal neurons synaptic plasticity.
format	article
author	Vincenzo Mastrolia Omar al Massadi Benoit de Pins Jean-Antoine Girault
author_facet	Vincenzo Mastrolia Omar al Massadi Benoit de Pins Jean-Antoine Girault
author_sort	Vincenzo Mastrolia
title	Pyk2 in dorsal hippocampus plays a selective role in spatial memory and synaptic plasticity
title_short	Pyk2 in dorsal hippocampus plays a selective role in spatial memory and synaptic plasticity
title_full	Pyk2 in dorsal hippocampus plays a selective role in spatial memory and synaptic plasticity
title_fullStr	Pyk2 in dorsal hippocampus plays a selective role in spatial memory and synaptic plasticity
title_full_unstemmed	Pyk2 in dorsal hippocampus plays a selective role in spatial memory and synaptic plasticity
title_sort	pyk2 in dorsal hippocampus plays a selective role in spatial memory and synaptic plasticity
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/25fc90fc77e143baa5cc1dd54097531e
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_version_	1718383981792067584

Pyk2 in dorsal hippocampus plays a selective role in spatial memory and synaptic plasticity

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