Folic acid-conjugated mesoporous silica nanoparticles for enhanced therapeutic efficacy of topotecan in retina cancers

Wei Qu,1 Bo Meng,1 Yangyang Yu,2 Shaowei Wang1 1Department of Ophthalmology, The 2nd Affiliated Hospital of Harbin Medical University, 2Department of Ophthalmology, Heilongjiang Provincial Hospital, Harbin, Heilongjiang, People’s Republic of China Abstract: In this study, topotecan-loade...

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Autores principales:	Qu W, Meng B, Yu Y, Wang S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Retinoblastoma topotecan mesoporous silica nanoparticles apoptosis anticancer effect Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/26005fd560f2466b878e5cccef23b32d
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spelling	oai:doaj.org-article:26005fd560f2466b878e5cccef23b32d2021-12-02T00:47:00ZFolic acid-conjugated mesoporous silica nanoparticles for enhanced therapeutic efficacy of topotecan in retina cancers1178-2013https://doaj.org/article/26005fd560f2466b878e5cccef23b32d2018-07-01T00:00:00Zhttps://www.dovepress.com/folic-acid-conjugated-mesoporous-silica-nanoparticles-for-enhanced-the-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Wei Qu,1 Bo Meng,1 Yangyang Yu,2 Shaowei Wang1 1Department of Ophthalmology, The 2nd Affiliated Hospital of Harbin Medical University, 2Department of Ophthalmology, Heilongjiang Provincial Hospital, Harbin, Heilongjiang, People’s Republic of China Abstract: In this study, topotecan-loaded mesoporous silica nanoparticles were prepared and surface conjugated with folic acid (FTMN) to enhance the therapeutic efficacy of topotecan for the treatment of retinoblastoma (RB) cancers. The particles were nano-sized and exhibited a sustained release of drug in the physiological conditions. The folic acid-conjugated nanoformulations exhibited a remarkable uptake in RB cells compared to that of non-targeted nanoparticles. These results clearly indicate that receptor-mediated endocytosis is the mechanism of cellular internalization. The greater cellular uptake of FTMN resulted in significantly higher cytotoxic effect in Y79 cancer cells compared to that of other formulations. The results were well corroborated with the live/dead assay and nuclear fragmentation assay. FTMN consistently induced apoptosis of cancer cells with an efficiency of ~58%. Our results clearly showed that nanoparticulate encapsulation of TPT exhibited superior anticancer efficacy in Y79 cancer cells compared to that of free drug or non-targeted nanoparticles. As expected, FTMN exhibited a remarkable reduction in the overall tumor volume compared to any other group with less presence of tumor cells in histology staining. Overall, folic acid-conjugated nanoparticulate system could provide an effective platform for RB treatment. Keywords: retinoblastoma, topotecan, mesoporous silica nanoparticles, apoptosis, anticancer effectQu WMeng BYu YWang SDove Medical PressarticleRetinoblastomatopotecanmesoporous silica nanoparticlesapoptosisanticancer effectMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 4379-4389 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Retinoblastoma topotecan mesoporous silica nanoparticles apoptosis anticancer effect Medicine (General) R5-920
spellingShingle	Retinoblastoma topotecan mesoporous silica nanoparticles apoptosis anticancer effect Medicine (General) R5-920 Qu W Meng B Yu Y Wang S Folic acid-conjugated mesoporous silica nanoparticles for enhanced therapeutic efficacy of topotecan in retina cancers
description	Wei Qu,1 Bo Meng,1 Yangyang Yu,2 Shaowei Wang1 1Department of Ophthalmology, The 2nd Affiliated Hospital of Harbin Medical University, 2Department of Ophthalmology, Heilongjiang Provincial Hospital, Harbin, Heilongjiang, People’s Republic of China Abstract: In this study, topotecan-loaded mesoporous silica nanoparticles were prepared and surface conjugated with folic acid (FTMN) to enhance the therapeutic efficacy of topotecan for the treatment of retinoblastoma (RB) cancers. The particles were nano-sized and exhibited a sustained release of drug in the physiological conditions. The folic acid-conjugated nanoformulations exhibited a remarkable uptake in RB cells compared to that of non-targeted nanoparticles. These results clearly indicate that receptor-mediated endocytosis is the mechanism of cellular internalization. The greater cellular uptake of FTMN resulted in significantly higher cytotoxic effect in Y79 cancer cells compared to that of other formulations. The results were well corroborated with the live/dead assay and nuclear fragmentation assay. FTMN consistently induced apoptosis of cancer cells with an efficiency of ~58%. Our results clearly showed that nanoparticulate encapsulation of TPT exhibited superior anticancer efficacy in Y79 cancer cells compared to that of free drug or non-targeted nanoparticles. As expected, FTMN exhibited a remarkable reduction in the overall tumor volume compared to any other group with less presence of tumor cells in histology staining. Overall, folic acid-conjugated nanoparticulate system could provide an effective platform for RB treatment. Keywords: retinoblastoma, topotecan, mesoporous silica nanoparticles, apoptosis, anticancer effect
format	article
author	Qu W Meng B Yu Y Wang S
author_facet	Qu W Meng B Yu Y Wang S
author_sort	Qu W
title	Folic acid-conjugated mesoporous silica nanoparticles for enhanced therapeutic efficacy of topotecan in retina cancers
title_short	Folic acid-conjugated mesoporous silica nanoparticles for enhanced therapeutic efficacy of topotecan in retina cancers
title_full	Folic acid-conjugated mesoporous silica nanoparticles for enhanced therapeutic efficacy of topotecan in retina cancers
title_fullStr	Folic acid-conjugated mesoporous silica nanoparticles for enhanced therapeutic efficacy of topotecan in retina cancers
title_full_unstemmed	Folic acid-conjugated mesoporous silica nanoparticles for enhanced therapeutic efficacy of topotecan in retina cancers
title_sort	folic acid-conjugated mesoporous silica nanoparticles for enhanced therapeutic efficacy of topotecan in retina cancers
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/26005fd560f2466b878e5cccef23b32d
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Folic acid-conjugated mesoporous silica nanoparticles for enhanced therapeutic efficacy of topotecan in retina cancers

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