Optimum protein requirement of juvenile orange-spotted grouper (Epinephelus coioides)

Abstract The purpose of subject was to explore the optimum protein requirement of juvenile grouper (Epinephelus coioides). In the test, 450 juveniles with an average weight (10.02 ± 0.22) g were randomly divided into six groups with triplicate, and were fed with 350, 400, 450, 500, 550 and 600 g/kg...

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Autores principales:	Xiaobo Yan, Junjiang Yang, Xiaohui Dong, Beiping Tan, Shuang Zhang, Shuyan Chi, QihuiYang, Hongyu Liu, Yuanzhi Yang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/260f69081271412eac5fbe5ce2d191dd
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spelling	oai:doaj.org-article:260f69081271412eac5fbe5ce2d191dd2021-12-02T11:39:39ZOptimum protein requirement of juvenile orange-spotted grouper (Epinephelus coioides)10.1038/s41598-021-85641-42045-2322https://doaj.org/article/260f69081271412eac5fbe5ce2d191dd2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85641-4https://doaj.org/toc/2045-2322Abstract The purpose of subject was to explore the optimum protein requirement of juvenile grouper (Epinephelus coioides). In the test, 450 juveniles with an average weight (10.02 ± 0.22) g were randomly divided into six groups with triplicate, and were fed with 350, 400, 450, 500, 550 and 600 g/kg iso-lipid test diet twice 1 day for 8 weeks, respectively. The results showed that: (1) With the increase of protein level, the body weight gain rate and specific growth rate first increased and then reduced, while the feed coefficient rate first decreased and then increased, while the protein efficiency significantly decreased (P < 0.05). (2) With the increase of protein level, the condition factor, hepaticsomatic index and visceralsomatic index significantly reduced (P < 0.05). (3) With the increase of protein level, the crude protein content of whole fish and muscle gradually increased, while the crude lipid content gradually decreased. (4) High-protein diet (550–600 g/kg) significantly increased the plasma total protein content and decreased the triglyceride content of orange-spotted grouper (P < 0.05). (5) Compared with the 350 g/kg group, 500, 550, 600 g/kg groups significantly increased the activities of glutamic-pyruvic transaminase and glutamic oxaloacetic transaminase in liver (P < 0.05). (6) With the increase of protein level, the protease activity of intestine first increased and then decreased, and reached the maximum at the protein level of 500 g/kg, while lipase and amylase decreased significantly (P < 0.05). (7) The activities of acid phosphatase, superoxide dismutase and lysozyme in liver increased first and then decreased with the increase of protein level, and reached the maximum in the 400 g/kg protein group. According to the analysis specific growth rate, the optimum protein level of juvenile orange-spotted grouper is 521.84 g/kg.Xiaobo YanJunjiang YangXiaohui DongBeiping TanShuang ZhangShuyan ChiQihuiYangHongyu LiuYuanzhi YangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaobo Yan Junjiang Yang Xiaohui Dong Beiping Tan Shuang Zhang Shuyan Chi QihuiYang Hongyu Liu Yuanzhi Yang Optimum protein requirement of juvenile orange-spotted grouper (Epinephelus coioides)
description	Abstract The purpose of subject was to explore the optimum protein requirement of juvenile grouper (Epinephelus coioides). In the test, 450 juveniles with an average weight (10.02 ± 0.22) g were randomly divided into six groups with triplicate, and were fed with 350, 400, 450, 500, 550 and 600 g/kg iso-lipid test diet twice 1 day for 8 weeks, respectively. The results showed that: (1) With the increase of protein level, the body weight gain rate and specific growth rate first increased and then reduced, while the feed coefficient rate first decreased and then increased, while the protein efficiency significantly decreased (P < 0.05). (2) With the increase of protein level, the condition factor, hepaticsomatic index and visceralsomatic index significantly reduced (P < 0.05). (3) With the increase of protein level, the crude protein content of whole fish and muscle gradually increased, while the crude lipid content gradually decreased. (4) High-protein diet (550–600 g/kg) significantly increased the plasma total protein content and decreased the triglyceride content of orange-spotted grouper (P < 0.05). (5) Compared with the 350 g/kg group, 500, 550, 600 g/kg groups significantly increased the activities of glutamic-pyruvic transaminase and glutamic oxaloacetic transaminase in liver (P < 0.05). (6) With the increase of protein level, the protease activity of intestine first increased and then decreased, and reached the maximum at the protein level of 500 g/kg, while lipase and amylase decreased significantly (P < 0.05). (7) The activities of acid phosphatase, superoxide dismutase and lysozyme in liver increased first and then decreased with the increase of protein level, and reached the maximum in the 400 g/kg protein group. According to the analysis specific growth rate, the optimum protein level of juvenile orange-spotted grouper is 521.84 g/kg.
format	article
author	Xiaobo Yan Junjiang Yang Xiaohui Dong Beiping Tan Shuang Zhang Shuyan Chi QihuiYang Hongyu Liu Yuanzhi Yang
author_facet	Xiaobo Yan Junjiang Yang Xiaohui Dong Beiping Tan Shuang Zhang Shuyan Chi QihuiYang Hongyu Liu Yuanzhi Yang
author_sort	Xiaobo Yan
title	Optimum protein requirement of juvenile orange-spotted grouper (Epinephelus coioides)
title_short	Optimum protein requirement of juvenile orange-spotted grouper (Epinephelus coioides)
title_full	Optimum protein requirement of juvenile orange-spotted grouper (Epinephelus coioides)
title_fullStr	Optimum protein requirement of juvenile orange-spotted grouper (Epinephelus coioides)
title_full_unstemmed	Optimum protein requirement of juvenile orange-spotted grouper (Epinephelus coioides)
title_sort	optimum protein requirement of juvenile orange-spotted grouper (epinephelus coioides)
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/260f69081271412eac5fbe5ce2d191dd
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Optimum protein requirement of juvenile orange-spotted grouper (Epinephelus coioides)

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