Cell fusing agent virus and dengue virus mutually interact in Aedes aegypti cell lines

Abstract The genus Flavivirus contains more than 70 single-stranded, positive-sense arthropod-borne RNA viruses. Some flaviviruses are particularly medically important to humans and other vertebrates including dengue virus (DENV), West Nile virus, and yellow fever virus. These viruses are transmitte...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Guangmei Zhang, Sultan Asad, Alexander A. Khromykh, Sassan Asgari
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2612d31f91d34fbdb7452002f51862d0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2612d31f91d34fbdb7452002f51862d0
record_format	dspace
spelling	oai:doaj.org-article:2612d31f91d34fbdb7452002f51862d02021-12-02T12:32:20ZCell fusing agent virus and dengue virus mutually interact in Aedes aegypti cell lines10.1038/s41598-017-07279-52045-2322https://doaj.org/article/2612d31f91d34fbdb7452002f51862d02017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07279-5https://doaj.org/toc/2045-2322Abstract The genus Flavivirus contains more than 70 single-stranded, positive-sense arthropod-borne RNA viruses. Some flaviviruses are particularly medically important to humans and other vertebrates including dengue virus (DENV), West Nile virus, and yellow fever virus. These viruses are transmitted to vertebrates by mosquitoes and other arthropod species. Mosquitoes are also infected by insect-specific flaviviruses (ISFs) that do not appear to be infective to vertebrates. Cell fusing agent virus (CFAV) was the first described ISF, which was discovered in an Aedes aegypti cell culture. We found that while CFAV infection could be significantly reduced by application of RNAi against the NS5 gene, removal of the treatment led to quick restoration of CFAV replication. Interestingly, we found that CFAV infection significantly enhanced replication of DENV, and vice versa, DENV infection significantly enhanced replication of CFAV in mosquito cells. We have shown that CFAV infection leads to increase in the expression of ribonuclease kappa (RNASEK), which is known to promote infection of viruses that rely on endocytosis and pH-dependent entry. Knockdown of RNASEK by dsRNA resulted in reduced DENV replication. Thus, increased expression of RNASEK induced by CFAV is likely to contribute to enhanced DENV replication in CFAV-infected cells.Guangmei ZhangSultan AsadAlexander A. KhromykhSassan AsgariNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Guangmei Zhang Sultan Asad Alexander A. Khromykh Sassan Asgari Cell fusing agent virus and dengue virus mutually interact in Aedes aegypti cell lines
description	Abstract The genus Flavivirus contains more than 70 single-stranded, positive-sense arthropod-borne RNA viruses. Some flaviviruses are particularly medically important to humans and other vertebrates including dengue virus (DENV), West Nile virus, and yellow fever virus. These viruses are transmitted to vertebrates by mosquitoes and other arthropod species. Mosquitoes are also infected by insect-specific flaviviruses (ISFs) that do not appear to be infective to vertebrates. Cell fusing agent virus (CFAV) was the first described ISF, which was discovered in an Aedes aegypti cell culture. We found that while CFAV infection could be significantly reduced by application of RNAi against the NS5 gene, removal of the treatment led to quick restoration of CFAV replication. Interestingly, we found that CFAV infection significantly enhanced replication of DENV, and vice versa, DENV infection significantly enhanced replication of CFAV in mosquito cells. We have shown that CFAV infection leads to increase in the expression of ribonuclease kappa (RNASEK), which is known to promote infection of viruses that rely on endocytosis and pH-dependent entry. Knockdown of RNASEK by dsRNA resulted in reduced DENV replication. Thus, increased expression of RNASEK induced by CFAV is likely to contribute to enhanced DENV replication in CFAV-infected cells.
format	article
author	Guangmei Zhang Sultan Asad Alexander A. Khromykh Sassan Asgari
author_facet	Guangmei Zhang Sultan Asad Alexander A. Khromykh Sassan Asgari
author_sort	Guangmei Zhang
title	Cell fusing agent virus and dengue virus mutually interact in Aedes aegypti cell lines
title_short	Cell fusing agent virus and dengue virus mutually interact in Aedes aegypti cell lines
title_full	Cell fusing agent virus and dengue virus mutually interact in Aedes aegypti cell lines
title_fullStr	Cell fusing agent virus and dengue virus mutually interact in Aedes aegypti cell lines
title_full_unstemmed	Cell fusing agent virus and dengue virus mutually interact in Aedes aegypti cell lines
title_sort	cell fusing agent virus and dengue virus mutually interact in aedes aegypti cell lines
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2612d31f91d34fbdb7452002f51862d0
work_keys_str_mv	AT guangmeizhang cellfusingagentvirusanddenguevirusmutuallyinteractinaedesaegypticelllines AT sultanasad cellfusingagentvirusanddenguevirusmutuallyinteractinaedesaegypticelllines AT alexanderakhromykh cellfusingagentvirusanddenguevirusmutuallyinteractinaedesaegypticelllines AT sassanasgari cellfusingagentvirusanddenguevirusmutuallyinteractinaedesaegypticelllines
_version_	1718394084279713792

Cell fusing agent virus and dengue virus mutually interact in Aedes aegypti cell lines

Ejemplares similares