Differential Gene Transcription in Honeybee (Apis cerana) Larvae Challenged by Chinese Sacbrood Virus (CSBV)

Honey bees are economically important social insect. They are suffering from all kinds of pathogens, especially the virus. In response to pathogens, different immune pathways such as Toll, Imd, Jak-Stat and RNAi are involved. In the present study, the transcription analysis of 32 immune-related gene...

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Autores principales:	Yi Zhang, Xiu Huang, ZaiFu Xu, RiChou Han, JingHua Chen
Formato:	article
Lenguaje:	EN
Publicado:	Universidade Estadual de Feira de Santana 2014
Materias:	Apis cerana, qRT – PCR, antiviral immunity, RNAi Zoology QL1-991 Ecology QH540-549.5 Natural history (General) QH1-278.5
Acceso en línea:	https://doaj.org/article/2618218dc79c4824a7e1d7f90699e3c4
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spelling	oai:doaj.org-article:2618218dc79c4824a7e1d7f90699e3c42021-12-02T13:21:21ZDifferential Gene Transcription in Honeybee (Apis cerana) Larvae Challenged by Chinese Sacbrood Virus (CSBV)0361-652510.13102/sociobiology.v60i4.413-420https://doaj.org/article/2618218dc79c4824a7e1d7f90699e3c42014-01-01T00:00:00Zhttp://periodicos.uefs.br/ojs/index.php/sociobiology/article/view/204https://doaj.org/toc/0361-6525Honey bees are economically important social insect. They are suffering from all kinds of pathogens, especially the virus. In response to pathogens, different immune pathways such as Toll, Imd, Jak-Stat and RNAi are involved. In the present study, the transcription analysis of 32 immune-related genes from <em>Apis cerana</em> challenged by Chinese sacbrood virus (CSBV), the most widely distributed virus in <em>A. cerana</em>, was carried out by qRT-PCR to provide cues for the antiviral mechanism and the effective control of bee viruses. The expression level of 22 genes were statistically changed, including 11 up-regulated genes (<em>catus</em>-<em>2</em>, <em>lys</em>-<em>2</em>, <em>vir</em>, <em>s3a</em>, <em>mta1</em>, <em>faa</em>, <em>vhdl</em>, <em>co-1-iv</em>, <em>ago</em>-<em>1</em>,<em> ago</em>-<em>3</em>, <em>aub</em>) in which 3 (<em>ago</em>-<em>1</em>, <em>ago</em>-<em>3</em>, <em>aub</em>) were related to RNAi pathway, and 11 down-regulated genes (<em>kenny</em>, <em>pgrp-lc</em>, <em>pgrp-s</em>2,<em> abaecin</em>,<em> lys</em>-<em>1</em>, <em>lys</em>-<em>3</em>, <em>domeless</em>, <em>tepa</em>, <em>mlc</em>, <em>dscam</em>, <em>rpl8</em>) related to Toll, Imd, and Jak-Stat pathways. The results indicated CSBV infection in <em>A. cerana</em> may activate a RNA-based antiviral immunity system. This work constituted the first report, under laboratory conditions, about induction of immune related genes in response to CSBV.Yi ZhangXiu HuangZaiFu XuRiChou HanJingHua ChenUniversidade Estadual de Feira de SantanaarticleApis cerana, qRT – PCR, antiviral immunity, RNAiZoologyQL1-991EcologyQH540-549.5Natural history (General)QH1-278.5ENSociobiology, Vol 60, Iss 4, Pp 413-420 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Apis cerana, qRT – PCR, antiviral immunity, RNAi Zoology QL1-991 Ecology QH540-549.5 Natural history (General) QH1-278.5
spellingShingle	Apis cerana, qRT – PCR, antiviral immunity, RNAi Zoology QL1-991 Ecology QH540-549.5 Natural history (General) QH1-278.5 Yi Zhang Xiu Huang ZaiFu Xu RiChou Han JingHua Chen Differential Gene Transcription in Honeybee (Apis cerana) Larvae Challenged by Chinese Sacbrood Virus (CSBV)
description	Honey bees are economically important social insect. They are suffering from all kinds of pathogens, especially the virus. In response to pathogens, different immune pathways such as Toll, Imd, Jak-Stat and RNAi are involved. In the present study, the transcription analysis of 32 immune-related genes from <em>Apis cerana</em> challenged by Chinese sacbrood virus (CSBV), the most widely distributed virus in <em>A. cerana</em>, was carried out by qRT-PCR to provide cues for the antiviral mechanism and the effective control of bee viruses. The expression level of 22 genes were statistically changed, including 11 up-regulated genes (<em>catus</em>-<em>2</em>, <em>lys</em>-<em>2</em>, <em>vir</em>, <em>s3a</em>, <em>mta1</em>, <em>faa</em>, <em>vhdl</em>, <em>co-1-iv</em>, <em>ago</em>-<em>1</em>,<em> ago</em>-<em>3</em>, <em>aub</em>) in which 3 (<em>ago</em>-<em>1</em>, <em>ago</em>-<em>3</em>, <em>aub</em>) were related to RNAi pathway, and 11 down-regulated genes (<em>kenny</em>, <em>pgrp-lc</em>, <em>pgrp-s</em>2,<em> abaecin</em>,<em> lys</em>-<em>1</em>, <em>lys</em>-<em>3</em>, <em>domeless</em>, <em>tepa</em>, <em>mlc</em>, <em>dscam</em>, <em>rpl8</em>) related to Toll, Imd, and Jak-Stat pathways. The results indicated CSBV infection in <em>A. cerana</em> may activate a RNA-based antiviral immunity system. This work constituted the first report, under laboratory conditions, about induction of immune related genes in response to CSBV.
format	article
author	Yi Zhang Xiu Huang ZaiFu Xu RiChou Han JingHua Chen
author_facet	Yi Zhang Xiu Huang ZaiFu Xu RiChou Han JingHua Chen
author_sort	Yi Zhang
title	Differential Gene Transcription in Honeybee (Apis cerana) Larvae Challenged by Chinese Sacbrood Virus (CSBV)
title_short	Differential Gene Transcription in Honeybee (Apis cerana) Larvae Challenged by Chinese Sacbrood Virus (CSBV)
title_full	Differential Gene Transcription in Honeybee (Apis cerana) Larvae Challenged by Chinese Sacbrood Virus (CSBV)
title_fullStr	Differential Gene Transcription in Honeybee (Apis cerana) Larvae Challenged by Chinese Sacbrood Virus (CSBV)
title_full_unstemmed	Differential Gene Transcription in Honeybee (Apis cerana) Larvae Challenged by Chinese Sacbrood Virus (CSBV)
title_sort	differential gene transcription in honeybee (apis cerana) larvae challenged by chinese sacbrood virus (csbv)
publisher	Universidade Estadual de Feira de Santana
publishDate	2014
url	https://doaj.org/article/2618218dc79c4824a7e1d7f90699e3c4
work_keys_str_mv	AT yizhang differentialgenetranscriptioninhoneybeeapisceranalarvaechallengedbychinesesacbroodviruscsbv AT xiuhuang differentialgenetranscriptioninhoneybeeapisceranalarvaechallengedbychinesesacbroodviruscsbv AT zaifuxu differentialgenetranscriptioninhoneybeeapisceranalarvaechallengedbychinesesacbroodviruscsbv AT richouhan differentialgenetranscriptioninhoneybeeapisceranalarvaechallengedbychinesesacbroodviruscsbv AT jinghuachen differentialgenetranscriptioninhoneybeeapisceranalarvaechallengedbychinesesacbroodviruscsbv
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Differential Gene Transcription in Honeybee (Apis cerana) Larvae Challenged by Chinese Sacbrood Virus (CSBV)

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