Investigation into the stability and culturability of Chinese enterotypes

Abstract Although many gut microbial enterotypes have been reported in Europe, Africa and the U.S., their effects on human health are still not yet clear. Culturing gut microbial enterotypes in vitro will be helpful to study their effects and applications. Here, fecal samples from 13 healthy Chinese...

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Autores principales:	Yeshi Yin, Bin Fan, Wei Liu, Rongrong Ren, Huahai Chen, Shaofeng Bai, Liying Zhu, Gang Sun, Yunsheng Yang, Xin Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/26210d7a192c4a338d55abda88928770
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spelling	oai:doaj.org-article:26210d7a192c4a338d55abda889287702021-12-02T16:05:59ZInvestigation into the stability and culturability of Chinese enterotypes10.1038/s41598-017-08478-w2045-2322https://doaj.org/article/26210d7a192c4a338d55abda889287702017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08478-whttps://doaj.org/toc/2045-2322Abstract Although many gut microbial enterotypes have been reported in Europe, Africa and the U.S., their effects on human health are still not yet clear. Culturing gut microbial enterotypes in vitro will be helpful to study their effects and applications. Here, fecal samples from 13 healthy Chinese volunteers were collected and subjected to next-generation sequencing. The results showed that seven of these samples belong to the Bacteroides enterotype and another six to the Prevotella enterotype. Stability of these Chinese gut microbial enterotypes was also evaluated. Results showed that most of the tested volunteer gut microbiota to be very stable. For one volunteer, the bacterial community returned to the state it was in before intestinal lavage and antibiotics treatment after four months. XP medium was found effective for simulating the Bacteroides enterotype independent of the original gut microbial community in an in vitro chemostat culture system. Although, the Prevotella enterotype was not very well simulated in vitro, different culture elements selectively enriched different gut bacteria. Pectin and xylan were found to be related to the enrichment of the genera Bacteroides, Sutterella, and Flavonifractor in this chemostat culture system.Yeshi YinBin FanWei LiuRongrong RenHuahai ChenShaofeng BaiLiying ZhuGang SunYunsheng YangXin WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yeshi Yin Bin Fan Wei Liu Rongrong Ren Huahai Chen Shaofeng Bai Liying Zhu Gang Sun Yunsheng Yang Xin Wang Investigation into the stability and culturability of Chinese enterotypes
description	Abstract Although many gut microbial enterotypes have been reported in Europe, Africa and the U.S., their effects on human health are still not yet clear. Culturing gut microbial enterotypes in vitro will be helpful to study their effects and applications. Here, fecal samples from 13 healthy Chinese volunteers were collected and subjected to next-generation sequencing. The results showed that seven of these samples belong to the Bacteroides enterotype and another six to the Prevotella enterotype. Stability of these Chinese gut microbial enterotypes was also evaluated. Results showed that most of the tested volunteer gut microbiota to be very stable. For one volunteer, the bacterial community returned to the state it was in before intestinal lavage and antibiotics treatment after four months. XP medium was found effective for simulating the Bacteroides enterotype independent of the original gut microbial community in an in vitro chemostat culture system. Although, the Prevotella enterotype was not very well simulated in vitro, different culture elements selectively enriched different gut bacteria. Pectin and xylan were found to be related to the enrichment of the genera Bacteroides, Sutterella, and Flavonifractor in this chemostat culture system.
format	article
author	Yeshi Yin Bin Fan Wei Liu Rongrong Ren Huahai Chen Shaofeng Bai Liying Zhu Gang Sun Yunsheng Yang Xin Wang
author_facet	Yeshi Yin Bin Fan Wei Liu Rongrong Ren Huahai Chen Shaofeng Bai Liying Zhu Gang Sun Yunsheng Yang Xin Wang
author_sort	Yeshi Yin
title	Investigation into the stability and culturability of Chinese enterotypes
title_short	Investigation into the stability and culturability of Chinese enterotypes
title_full	Investigation into the stability and culturability of Chinese enterotypes
title_fullStr	Investigation into the stability and culturability of Chinese enterotypes
title_full_unstemmed	Investigation into the stability and culturability of Chinese enterotypes
title_sort	investigation into the stability and culturability of chinese enterotypes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/26210d7a192c4a338d55abda88928770
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Investigation into the stability and culturability of Chinese enterotypes

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