On the shape and structure of the murine pulmonary heart valve

Abstract Murine animal models are an established standard in translational research and provides a potential platform for studying heart valve disease. To date, studies on heart valve disease using murine models have been hindered by a lack of appropriate methodologies due to their small scale. In t...

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Autores principales:	Yifei Liu, Xinzeng Feng, Hao Liu, David W. McComb, Christopher K. Breuer, Michael S. Sacks
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2622d5b5e0b140f4aadaecd9ce8af04e
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spelling	oai:doaj.org-article:2622d5b5e0b140f4aadaecd9ce8af04e2021-12-02T16:14:46ZOn the shape and structure of the murine pulmonary heart valve10.1038/s41598-021-93513-02045-2322https://doaj.org/article/2622d5b5e0b140f4aadaecd9ce8af04e2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93513-0https://doaj.org/toc/2045-2322Abstract Murine animal models are an established standard in translational research and provides a potential platform for studying heart valve disease. To date, studies on heart valve disease using murine models have been hindered by a lack of appropriate methodologies due to their small scale. In the present study, we developed a multi-scale, imaging-based approach to extract the functional structure and geometry for the murine heart valve. We chose the pulmonary valve (PV) to study, due to its importance in congenital heart valve disease. Excised pulmonary outflow tracts from eleven 1-year old C57BL/6J mice were fixed at 10, 20, and 30 mmHg to simulate physiological loading. Micro-computed tomography was used to reconstruct the 3D organ-level PV geometry, which was then spatially correlated with serial en-face scanning electron microscopy imaging to quantify local collagen fiber distributions. From the acquired volume renderings, we obtained the geometric descriptors of the murine PV under increasing transvalvular pressures, which demonstrated remarkable consistency. Results to date suggest that the preferred collagen orientation was predominantly in the circumferential direction, as in larger mammalian valves. The present study represents a first step in establishing organ-level murine models for the study of heart valve disease.Yifei LiuXinzeng FengHao LiuDavid W. McCombChristopher K. BreuerMichael S. SacksNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yifei Liu Xinzeng Feng Hao Liu David W. McComb Christopher K. Breuer Michael S. Sacks On the shape and structure of the murine pulmonary heart valve
description	Abstract Murine animal models are an established standard in translational research and provides a potential platform for studying heart valve disease. To date, studies on heart valve disease using murine models have been hindered by a lack of appropriate methodologies due to their small scale. In the present study, we developed a multi-scale, imaging-based approach to extract the functional structure and geometry for the murine heart valve. We chose the pulmonary valve (PV) to study, due to its importance in congenital heart valve disease. Excised pulmonary outflow tracts from eleven 1-year old C57BL/6J mice were fixed at 10, 20, and 30 mmHg to simulate physiological loading. Micro-computed tomography was used to reconstruct the 3D organ-level PV geometry, which was then spatially correlated with serial en-face scanning electron microscopy imaging to quantify local collagen fiber distributions. From the acquired volume renderings, we obtained the geometric descriptors of the murine PV under increasing transvalvular pressures, which demonstrated remarkable consistency. Results to date suggest that the preferred collagen orientation was predominantly in the circumferential direction, as in larger mammalian valves. The present study represents a first step in establishing organ-level murine models for the study of heart valve disease.
format	article
author	Yifei Liu Xinzeng Feng Hao Liu David W. McComb Christopher K. Breuer Michael S. Sacks
author_facet	Yifei Liu Xinzeng Feng Hao Liu David W. McComb Christopher K. Breuer Michael S. Sacks
author_sort	Yifei Liu
title	On the shape and structure of the murine pulmonary heart valve
title_short	On the shape and structure of the murine pulmonary heart valve
title_full	On the shape and structure of the murine pulmonary heart valve
title_fullStr	On the shape and structure of the murine pulmonary heart valve
title_full_unstemmed	On the shape and structure of the murine pulmonary heart valve
title_sort	on the shape and structure of the murine pulmonary heart valve
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2622d5b5e0b140f4aadaecd9ce8af04e
work_keys_str_mv	AT yifeiliu ontheshapeandstructureofthemurinepulmonaryheartvalve AT xinzengfeng ontheshapeandstructureofthemurinepulmonaryheartvalve AT haoliu ontheshapeandstructureofthemurinepulmonaryheartvalve AT davidwmccomb ontheshapeandstructureofthemurinepulmonaryheartvalve AT christopherkbreuer ontheshapeandstructureofthemurinepulmonaryheartvalve AT michaelssacks ontheshapeandstructureofthemurinepulmonaryheartvalve
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On the shape and structure of the murine pulmonary heart valve

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