Transcriptome Analysis and Expression Profiling of Molecular Responses to Cd Toxicity in Morchella spongiola

Morchella is a genus of fungi with the ability to concentrate Cd both in the fruit-body and mycelium. However, the molecular mechanisms conferring resistance to Cd stress in Morchella are unknown. Here, RNA-based transcriptomic sequencing was used to identify the genes and pathways involved in Cd to...

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Autores principales:	Xu Hongyan, Xie Zhanling, Jiang Hongchen, Guo Jing, Meng Qing, Zhao Yuan, Wang Xiaofang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	cadmium stress transporter protein enzymatic antioxidant non-enzymatic antioxidant gene regulatory network Botany QK1-989
Acceso en línea:	https://doaj.org/article/26257a1c20ad4fc6b862d333aed86638
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spelling	oai:doaj.org-article:26257a1c20ad4fc6b862d333aed866382021-12-01T14:40:58ZTranscriptome Analysis and Expression Profiling of Molecular Responses to Cd Toxicity in Morchella spongiola1229-80932092-932310.1080/12298093.2021.1937882https://doaj.org/article/26257a1c20ad4fc6b862d333aed866382021-07-01T00:00:00Zhttp://dx.doi.org/10.1080/12298093.2021.1937882https://doaj.org/toc/1229-8093https://doaj.org/toc/2092-9323Morchella is a genus of fungi with the ability to concentrate Cd both in the fruit-body and mycelium. However, the molecular mechanisms conferring resistance to Cd stress in Morchella are unknown. Here, RNA-based transcriptomic sequencing was used to identify the genes and pathways involved in Cd tolerance in Morchella spongiola. 7444 differentially expressed genes (DEGs) were identified by cultivating M. spongiola in media containing 0.15, 0.90, or 1.50 mg/L Cd2+. The DEGs were divided into six sub-clusters based on their global expression profiles. GO enrichment analysis indicated that numerous DEGs were associated with catalytic activity, cell cycle control, and the ribosome. KEGG enrichment analysis showed that the main pathways under Cd stress were MAPK signaling, oxidative phosphorylation, pyruvate metabolism, and propanoate metabolism. In addition, several DEGs encoding ion transporters, enzymatic/non-enzymatic antioxidants, and transcription factors were identified. Based on these results, a preliminary gene regulatory network was firstly proposed to illustrate the molecular mechanisms of Cd detoxification in M. spongiola. These results provide valuable insights into the Cd tolerance mechanism of M. spongiola and constitute a robust foundation for further studies on detoxification mechanisms in macrofungi that could potentially lead to the development of new and improved fungal bioremediation strategies.Xu HongyanXie ZhanlingJiang HongchenGuo JingMeng QingZhao YuanWang XiaofangTaylor & Francis Grouparticlecadmium stresstransporter proteinenzymatic antioxidantnon-enzymatic antioxidantgene regulatory networkBotanyQK1-989ENMycobiology, Vol 49, Iss 4, Pp 421-433 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cadmium stress transporter protein enzymatic antioxidant non-enzymatic antioxidant gene regulatory network Botany QK1-989
spellingShingle	cadmium stress transporter protein enzymatic antioxidant non-enzymatic antioxidant gene regulatory network Botany QK1-989 Xu Hongyan Xie Zhanling Jiang Hongchen Guo Jing Meng Qing Zhao Yuan Wang Xiaofang Transcriptome Analysis and Expression Profiling of Molecular Responses to Cd Toxicity in Morchella spongiola
description	Morchella is a genus of fungi with the ability to concentrate Cd both in the fruit-body and mycelium. However, the molecular mechanisms conferring resistance to Cd stress in Morchella are unknown. Here, RNA-based transcriptomic sequencing was used to identify the genes and pathways involved in Cd tolerance in Morchella spongiola. 7444 differentially expressed genes (DEGs) were identified by cultivating M. spongiola in media containing 0.15, 0.90, or 1.50 mg/L Cd2+. The DEGs were divided into six sub-clusters based on their global expression profiles. GO enrichment analysis indicated that numerous DEGs were associated with catalytic activity, cell cycle control, and the ribosome. KEGG enrichment analysis showed that the main pathways under Cd stress were MAPK signaling, oxidative phosphorylation, pyruvate metabolism, and propanoate metabolism. In addition, several DEGs encoding ion transporters, enzymatic/non-enzymatic antioxidants, and transcription factors were identified. Based on these results, a preliminary gene regulatory network was firstly proposed to illustrate the molecular mechanisms of Cd detoxification in M. spongiola. These results provide valuable insights into the Cd tolerance mechanism of M. spongiola and constitute a robust foundation for further studies on detoxification mechanisms in macrofungi that could potentially lead to the development of new and improved fungal bioremediation strategies.
format	article
author	Xu Hongyan Xie Zhanling Jiang Hongchen Guo Jing Meng Qing Zhao Yuan Wang Xiaofang
author_facet	Xu Hongyan Xie Zhanling Jiang Hongchen Guo Jing Meng Qing Zhao Yuan Wang Xiaofang
author_sort	Xu Hongyan
title	Transcriptome Analysis and Expression Profiling of Molecular Responses to Cd Toxicity in Morchella spongiola
title_short	Transcriptome Analysis and Expression Profiling of Molecular Responses to Cd Toxicity in Morchella spongiola
title_full	Transcriptome Analysis and Expression Profiling of Molecular Responses to Cd Toxicity in Morchella spongiola
title_fullStr	Transcriptome Analysis and Expression Profiling of Molecular Responses to Cd Toxicity in Morchella spongiola
title_full_unstemmed	Transcriptome Analysis and Expression Profiling of Molecular Responses to Cd Toxicity in Morchella spongiola
title_sort	transcriptome analysis and expression profiling of molecular responses to cd toxicity in morchella spongiola
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/26257a1c20ad4fc6b862d333aed86638
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Transcriptome Analysis and Expression Profiling of Molecular Responses to Cd Toxicity in Morchella spongiola

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