Role of androgen receptor CAG repeat polymorphism and X-inactivation in the manifestation of recurrent spontaneous abortions in Indian women.

The aim of the present study was to investigate the role of CAG repeat polymorphism and X-chromosome Inactivation (XCI) pattern in Recurrent Spontaneous Abortions among Indian women which has not been hitherto explored. 117 RSA cases and 224 Controls were included in the study. Cases were recruited...

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Autores principales:	Meka Aruna, Shilpi Dasgupta, Pisapati V S Sirisha, Sadaranga Andal Bhaskar, Surapaneni Tarakeswari, Lalji Singh, B Mohan Reddy
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/26306fdf88c44f08b5f7d7dc53f032e9
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spelling	oai:doaj.org-article:26306fdf88c44f08b5f7d7dc53f032e92021-11-18T06:57:20ZRole of androgen receptor CAG repeat polymorphism and X-inactivation in the manifestation of recurrent spontaneous abortions in Indian women.1932-620310.1371/journal.pone.0017718https://doaj.org/article/26306fdf88c44f08b5f7d7dc53f032e92011-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21423805/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The aim of the present study was to investigate the role of CAG repeat polymorphism and X-chromosome Inactivation (XCI) pattern in Recurrent Spontaneous Abortions among Indian women which has not been hitherto explored. 117 RSA cases and 224 Controls were included in the study. Cases were recruited from two different hospitals--Lakshmi Fertility Clinic, Nellore and Fernandez Maternity Hospital, Hyderabad. Controls were roughly matched for age, ethnicity and socioeconomic status. The CAG repeats of the Androgen Receptor gene were genotyped using a PCR-based assay and were analysed using the GeneMapper software to determine the CAG repeat length. XCI analysis was also carried out to assess the inactivation percentages. RSA cases had a significantly greater frequency of allele sizes in the polymorphic range above 19 repeats (p = 0.006), which is the median value of the controls, and in the biallelic mean range above 21 repeats (p = 0.002). We found no evidence of abnormal incidence of skewed X-inactivation. We conclude that longer CAG repeat lengths are associated with increased odds for RSA with statistical power estimated to be ∼90%.Meka ArunaShilpi DasguptaPisapati V S SirishaSadaranga Andal BhaskarSurapaneni TarakeswariLalji SinghB Mohan ReddyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 3, p e17718 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Meka Aruna Shilpi Dasgupta Pisapati V S Sirisha Sadaranga Andal Bhaskar Surapaneni Tarakeswari Lalji Singh B Mohan Reddy Role of androgen receptor CAG repeat polymorphism and X-inactivation in the manifestation of recurrent spontaneous abortions in Indian women.
description	The aim of the present study was to investigate the role of CAG repeat polymorphism and X-chromosome Inactivation (XCI) pattern in Recurrent Spontaneous Abortions among Indian women which has not been hitherto explored. 117 RSA cases and 224 Controls were included in the study. Cases were recruited from two different hospitals--Lakshmi Fertility Clinic, Nellore and Fernandez Maternity Hospital, Hyderabad. Controls were roughly matched for age, ethnicity and socioeconomic status. The CAG repeats of the Androgen Receptor gene were genotyped using a PCR-based assay and were analysed using the GeneMapper software to determine the CAG repeat length. XCI analysis was also carried out to assess the inactivation percentages. RSA cases had a significantly greater frequency of allele sizes in the polymorphic range above 19 repeats (p = 0.006), which is the median value of the controls, and in the biallelic mean range above 21 repeats (p = 0.002). We found no evidence of abnormal incidence of skewed X-inactivation. We conclude that longer CAG repeat lengths are associated with increased odds for RSA with statistical power estimated to be ∼90%.
format	article
author	Meka Aruna Shilpi Dasgupta Pisapati V S Sirisha Sadaranga Andal Bhaskar Surapaneni Tarakeswari Lalji Singh B Mohan Reddy
author_facet	Meka Aruna Shilpi Dasgupta Pisapati V S Sirisha Sadaranga Andal Bhaskar Surapaneni Tarakeswari Lalji Singh B Mohan Reddy
author_sort	Meka Aruna
title	Role of androgen receptor CAG repeat polymorphism and X-inactivation in the manifestation of recurrent spontaneous abortions in Indian women.
title_short	Role of androgen receptor CAG repeat polymorphism and X-inactivation in the manifestation of recurrent spontaneous abortions in Indian women.
title_full	Role of androgen receptor CAG repeat polymorphism and X-inactivation in the manifestation of recurrent spontaneous abortions in Indian women.
title_fullStr	Role of androgen receptor CAG repeat polymorphism and X-inactivation in the manifestation of recurrent spontaneous abortions in Indian women.
title_full_unstemmed	Role of androgen receptor CAG repeat polymorphism and X-inactivation in the manifestation of recurrent spontaneous abortions in Indian women.
title_sort	role of androgen receptor cag repeat polymorphism and x-inactivation in the manifestation of recurrent spontaneous abortions in indian women.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/26306fdf88c44f08b5f7d7dc53f032e9
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Role of androgen receptor CAG repeat polymorphism and X-inactivation in the manifestation of recurrent spontaneous abortions in Indian women.

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