HCV eradication with DAAs differently affects HIV males and females: A whole miRNA sequencing characterization
Gender-specific consequences after HCV eradication are unexplored. MicroRNAs (miRNAs) play a crucial role in the immune response against viral infections. However, few have highlighted miRNA role in sex-biased disease or therapy response. We aim to assess gender differences reflected in the miRNA ex...
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2022
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oai:doaj.org-article:26319812b14e4a5ea859ebf8db1d51b62021-11-14T04:30:30ZHCV eradication with DAAs differently affects HIV males and females: A whole miRNA sequencing characterization0753-332210.1016/j.biopha.2021.112405https://doaj.org/article/26319812b14e4a5ea859ebf8db1d51b62022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0753332221011914https://doaj.org/toc/0753-3322Gender-specific consequences after HCV eradication are unexplored. MicroRNAs (miRNAs) play a crucial role in the immune response against viral infections. However, few have highlighted miRNA role in sex-biased disease or therapy response. We aim to assess gender differences reflected in the miRNA expression of HIV/HCV-coinfected patients who achieve sustained virological response (SVR) with direct acting antivirals (DAAs). We conducted a prospective study of miRNA expression in PBMCs from 28 chronic HIV/HCV-coinfected patients (HIV/HCV) at baseline and after achieving SVR with DAAs. Sixteen HIV-monoinfected patients (HIV) and 36 healthy controls (HC) were used as controls. Identification of significant differentially expressed (SDE) miRNAs was performed with generalized linear model and mixed GLMs. We also explored putative dysregulated biological pathways. At baseline, the HIV/HCV patients showed differences in the miRNA profile concerning the HIV group (165 and 102 SDE miRNAs for males and females, respectively). Gender-stratified analysis of HIV/HCV group at baseline versus at SVR achievement showed higher differences in males (80 SDE miRNAs) than in females (55 SDE miRNAs). After SVR, HIV/HCV group showed similar values to HIV individuals, especially in females (1 SDE miRNA). However, ten miRNAs in males remained dysregulated, which were mainly involved in cancer, fatty acid, and inflammatory pathways. Taken together, our results show gender-biased dysregulation in the miRNA expression profile of PBMCs after HCV eradication with DAAs. These differences were normalized in females, while miRNA profile and their target-related pathways in males lack of normalization, which may be related to a high-risk of developing liver-related complications.Daniel Valle-MillaresÓscar Brochado-KithAlicia Gómez-SanzLuz Martín-CarboneroPablo RyanIgnacio De los SantosJuan M. CastroJesús TroyaMario Mayoral-MuñozGuillermo CuevasPaula Martínez-RománJesús Sanz-SanzMaría Muñoz-MuñozMaría Á Jiménez-SousaSalvador ResinoVerónica BrizAmanda Fernández-RodríguezElsevierarticleHCVHIVMicroRNAsDAAsGenderHigh throughput sequencingTherapeutics. PharmacologyRM1-950ENBiomedicine & Pharmacotherapy, Vol 145, Iss , Pp 112405- (2022) |
| institution |
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| collection |
DOAJ |
| language |
EN |
| topic |
HCV HIV MicroRNAs DAAs Gender High throughput sequencing Therapeutics. Pharmacology RM1-950 |
| spellingShingle |
HCV HIV MicroRNAs DAAs Gender High throughput sequencing Therapeutics. Pharmacology RM1-950 Daniel Valle-Millares Óscar Brochado-Kith Alicia Gómez-Sanz Luz Martín-Carbonero Pablo Ryan Ignacio De los Santos Juan M. Castro Jesús Troya Mario Mayoral-Muñoz Guillermo Cuevas Paula Martínez-Román Jesús Sanz-Sanz María Muñoz-Muñoz María Á Jiménez-Sousa Salvador Resino Verónica Briz Amanda Fernández-Rodríguez HCV eradication with DAAs differently affects HIV males and females: A whole miRNA sequencing characterization |
| description |
Gender-specific consequences after HCV eradication are unexplored. MicroRNAs (miRNAs) play a crucial role in the immune response against viral infections. However, few have highlighted miRNA role in sex-biased disease or therapy response. We aim to assess gender differences reflected in the miRNA expression of HIV/HCV-coinfected patients who achieve sustained virological response (SVR) with direct acting antivirals (DAAs). We conducted a prospective study of miRNA expression in PBMCs from 28 chronic HIV/HCV-coinfected patients (HIV/HCV) at baseline and after achieving SVR with DAAs. Sixteen HIV-monoinfected patients (HIV) and 36 healthy controls (HC) were used as controls. Identification of significant differentially expressed (SDE) miRNAs was performed with generalized linear model and mixed GLMs. We also explored putative dysregulated biological pathways. At baseline, the HIV/HCV patients showed differences in the miRNA profile concerning the HIV group (165 and 102 SDE miRNAs for males and females, respectively). Gender-stratified analysis of HIV/HCV group at baseline versus at SVR achievement showed higher differences in males (80 SDE miRNAs) than in females (55 SDE miRNAs). After SVR, HIV/HCV group showed similar values to HIV individuals, especially in females (1 SDE miRNA). However, ten miRNAs in males remained dysregulated, which were mainly involved in cancer, fatty acid, and inflammatory pathways. Taken together, our results show gender-biased dysregulation in the miRNA expression profile of PBMCs after HCV eradication with DAAs. These differences were normalized in females, while miRNA profile and their target-related pathways in males lack of normalization, which may be related to a high-risk of developing liver-related complications. |
| format |
article |
| author |
Daniel Valle-Millares Óscar Brochado-Kith Alicia Gómez-Sanz Luz Martín-Carbonero Pablo Ryan Ignacio De los Santos Juan M. Castro Jesús Troya Mario Mayoral-Muñoz Guillermo Cuevas Paula Martínez-Román Jesús Sanz-Sanz María Muñoz-Muñoz María Á Jiménez-Sousa Salvador Resino Verónica Briz Amanda Fernández-Rodríguez |
| author_facet |
Daniel Valle-Millares Óscar Brochado-Kith Alicia Gómez-Sanz Luz Martín-Carbonero Pablo Ryan Ignacio De los Santos Juan M. Castro Jesús Troya Mario Mayoral-Muñoz Guillermo Cuevas Paula Martínez-Román Jesús Sanz-Sanz María Muñoz-Muñoz María Á Jiménez-Sousa Salvador Resino Verónica Briz Amanda Fernández-Rodríguez |
| author_sort |
Daniel Valle-Millares |
| title |
HCV eradication with DAAs differently affects HIV males and females: A whole miRNA sequencing characterization |
| title_short |
HCV eradication with DAAs differently affects HIV males and females: A whole miRNA sequencing characterization |
| title_full |
HCV eradication with DAAs differently affects HIV males and females: A whole miRNA sequencing characterization |
| title_fullStr |
HCV eradication with DAAs differently affects HIV males and females: A whole miRNA sequencing characterization |
| title_full_unstemmed |
HCV eradication with DAAs differently affects HIV males and females: A whole miRNA sequencing characterization |
| title_sort |
hcv eradication with daas differently affects hiv males and females: a whole mirna sequencing characterization |
| publisher |
Elsevier |
| publishDate |
2022 |
| url |
https://doaj.org/article/26319812b14e4a5ea859ebf8db1d51b6 |
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