β-Cyclocitral, a Master Regulator of Multiple Stress-Responsive Genes in <i>Solanum lycopersicum</i> L. Plants

β-cyclocitral (βCC), a major apocarotenoid of β-carotene, enhances plants’ defense against environmental stresses. However, the knowledge of βCC’s involvement in the complex stress-signaling network is limited. Here we demonstrate how βCC reprograms the transcriptional responses that enable <i>...

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Autores principales:	Shreyas Deshpande, Vishwabandhu Purkar, Sirsha Mitra
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	apocarotenoids phytohormone plant defense reactive oxygen species singlet oxygen Botany QK1-989
Acceso en línea:	https://doaj.org/article/26334050fa404239a35c2e85b40aabf4
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spelling	oai:doaj.org-article:26334050fa404239a35c2e85b40aabf42021-11-25T18:46:48Zβ-Cyclocitral, a Master Regulator of Multiple Stress-Responsive Genes in <i>Solanum lycopersicum</i> L. Plants10.3390/plants101124652223-7747https://doaj.org/article/26334050fa404239a35c2e85b40aabf42021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2465https://doaj.org/toc/2223-7747β-cyclocitral (βCC), a major apocarotenoid of β-carotene, enhances plants’ defense against environmental stresses. However, the knowledge of βCC’s involvement in the complex stress-signaling network is limited. Here we demonstrate how βCC reprograms the transcriptional responses that enable <i>Solanum lycopersicum</i> L. (tomato) plants to endure a plethora of environmental stresses. Comparative transcriptome analysis of control and βCC-treated tomato plants was done by generating RNA sequences in the BGISEQ-500 platform. The trimmed sequences were mapped on the tomato reference genome that identifies 211 protein-coding differentially expressed genes. Gene ontology and Kyoto Encyclopedia of Genes and Genomes analysis and their enrichment uncovered that only upregulated genes are attributed to the stress response. Moreover, 80% of the upregulated genes are functionally related to abiotic and biotic stresses. Co-functional analysis of stress-responsive genes revealed a network of 18 genes that code for heat shock proteins, transcription factors (TFs), and calcium-binding proteins. The upregulation of jasmonic acid (JA)-dependent TFs (<i>MYC2</i>, <i>MYB44</i>, <i>ERFs</i>) but not the JA biosynthetic genes is surprising. However, the upregulation of <i>DREB3</i>, an abscisic acid (ABA)-independent TF, validates the unaltered expression of ABA biosynthetic genes. We conclude that βCC treatment upregulates multiple stress-responsive genes without eliciting JA and ABA biosynthesis.Shreyas DeshpandeVishwabandhu PurkarSirsha MitraMDPI AGarticleapocarotenoidsphytohormoneplant defensereactive oxygen speciessinglet oxygenBotanyQK1-989ENPlants, Vol 10, Iss 2465, p 2465 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	apocarotenoids phytohormone plant defense reactive oxygen species singlet oxygen Botany QK1-989
spellingShingle	apocarotenoids phytohormone plant defense reactive oxygen species singlet oxygen Botany QK1-989 Shreyas Deshpande Vishwabandhu Purkar Sirsha Mitra β-Cyclocitral, a Master Regulator of Multiple Stress-Responsive Genes in <i>Solanum lycopersicum</i> L. Plants
description	β-cyclocitral (βCC), a major apocarotenoid of β-carotene, enhances plants’ defense against environmental stresses. However, the knowledge of βCC’s involvement in the complex stress-signaling network is limited. Here we demonstrate how βCC reprograms the transcriptional responses that enable <i>Solanum lycopersicum</i> L. (tomato) plants to endure a plethora of environmental stresses. Comparative transcriptome analysis of control and βCC-treated tomato plants was done by generating RNA sequences in the BGISEQ-500 platform. The trimmed sequences were mapped on the tomato reference genome that identifies 211 protein-coding differentially expressed genes. Gene ontology and Kyoto Encyclopedia of Genes and Genomes analysis and their enrichment uncovered that only upregulated genes are attributed to the stress response. Moreover, 80% of the upregulated genes are functionally related to abiotic and biotic stresses. Co-functional analysis of stress-responsive genes revealed a network of 18 genes that code for heat shock proteins, transcription factors (TFs), and calcium-binding proteins. The upregulation of jasmonic acid (JA)-dependent TFs (<i>MYC2</i>, <i>MYB44</i>, <i>ERFs</i>) but not the JA biosynthetic genes is surprising. However, the upregulation of <i>DREB3</i>, an abscisic acid (ABA)-independent TF, validates the unaltered expression of ABA biosynthetic genes. We conclude that βCC treatment upregulates multiple stress-responsive genes without eliciting JA and ABA biosynthesis.
format	article
author	Shreyas Deshpande Vishwabandhu Purkar Sirsha Mitra
author_facet	Shreyas Deshpande Vishwabandhu Purkar Sirsha Mitra
author_sort	Shreyas Deshpande
title	β-Cyclocitral, a Master Regulator of Multiple Stress-Responsive Genes in <i>Solanum lycopersicum</i> L. Plants
title_short	β-Cyclocitral, a Master Regulator of Multiple Stress-Responsive Genes in <i>Solanum lycopersicum</i> L. Plants
title_full	β-Cyclocitral, a Master Regulator of Multiple Stress-Responsive Genes in <i>Solanum lycopersicum</i> L. Plants
title_fullStr	β-Cyclocitral, a Master Regulator of Multiple Stress-Responsive Genes in <i>Solanum lycopersicum</i> L. Plants
title_full_unstemmed	β-Cyclocitral, a Master Regulator of Multiple Stress-Responsive Genes in <i>Solanum lycopersicum</i> L. Plants
title_sort	β-cyclocitral, a master regulator of multiple stress-responsive genes in <i>solanum lycopersicum</i> l. plants
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/26334050fa404239a35c2e85b40aabf4
work_keys_str_mv	AT shreyasdeshpande bcyclocitralamasterregulatorofmultiplestressresponsivegenesinisolanumlycopersicumilplants AT vishwabandhupurkar bcyclocitralamasterregulatorofmultiplestressresponsivegenesinisolanumlycopersicumilplants AT sirshamitra bcyclocitralamasterregulatorofmultiplestressresponsivegenesinisolanumlycopersicumilplants
_version_	1718410699972018176

β-Cyclocitral, a Master Regulator of Multiple Stress-Responsive Genes in <i>Solanum lycopersicum</i> L. Plants

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