Impact of obesity on the expression profile of natriuretic peptide system in a rat experimental model.

Natriuretic peptides (NPs) play an important role in obesity and aim of this study was to evaluate, in cardiac tissue of obese Zucker rats (O, n = 29) their transcriptomic profile compared to controls (CO, n = 24) by Real-Time PCR study; CNP protein expression was evaluated by immunostaining and imm...

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Autores principales:	Manuela Cabiati, Serena Raucci, Tiziana Liistro, Eugenia Belcastro, Tommaso Prescimone, Chiara Caselli, Marco Matteucci, Patricia Iozzo, Letizia Mattii, Daniela Giannessi, Silvia Del Ry
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/263651c52d5f4319a3c5c52d36ba0591
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spelling	oai:doaj.org-article:263651c52d5f4319a3c5c52d36ba05912021-11-18T08:57:43ZImpact of obesity on the expression profile of natriuretic peptide system in a rat experimental model.1932-620310.1371/journal.pone.0072959https://doaj.org/article/263651c52d5f4319a3c5c52d36ba05912013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24009719/?tool=EBIhttps://doaj.org/toc/1932-6203Natriuretic peptides (NPs) play an important role in obesity and aim of this study was to evaluate, in cardiac tissue of obese Zucker rats (O, n = 29) their transcriptomic profile compared to controls (CO, n = 24) by Real-Time PCR study; CNP protein expression was evaluated by immunostaining and immunometric tests. Myocardial histology was performed, confirming no alteration of organ structure. While ANP and BNP are cardiac peptides, CNP is mainly an endothelial hormone; thus its expression, as well as that of NPR-B and NPR-C, was also evaluated in kidney and lung of an animal subgroup (n = 20). In heart, lower BNP mRNA levels in O vs CO (p = 0.02) as well as ANP and CNP (p = ns), were detected. NPR-B/NPR-A mRNA was similar in O and CO, while NPR-C was numerically lower (p = ns) in O than in CO. In kidney, CNP/NPR-B/NPR-C mRNA was similar in O and CO, while in lung CNP/NPR-C expression decreased and NPR-B increased (p = ns) in O vs CO. Subdividing into fasting and hyperglycemic rats, the pattern of mRNA expression for each gene analyzed remained unchanged. The trend observed in heart, kidney and lung for CNP protein concentrations and immunohistochemistry reflected the mRNA expression. TNF-α and IL-6 mRNA were measured in each tissue and no significant genotype effect was detected in any tissue. The main NP variations were observed at the cardiac level, suggesting a reduced release by cardiac cells. The understanding of mechanisms involved in the modulation of the NP system in obesity could be a useful starting point for future clinical study devoted to identifying new obesity treatment strategies.Manuela CabiatiSerena RaucciTiziana LiistroEugenia BelcastroTommaso PrescimoneChiara CaselliMarco MatteucciPatricia IozzoLetizia MattiiDaniela GiannessiSilvia Del RyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e72959 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Manuela Cabiati Serena Raucci Tiziana Liistro Eugenia Belcastro Tommaso Prescimone Chiara Caselli Marco Matteucci Patricia Iozzo Letizia Mattii Daniela Giannessi Silvia Del Ry Impact of obesity on the expression profile of natriuretic peptide system in a rat experimental model.
description	Natriuretic peptides (NPs) play an important role in obesity and aim of this study was to evaluate, in cardiac tissue of obese Zucker rats (O, n = 29) their transcriptomic profile compared to controls (CO, n = 24) by Real-Time PCR study; CNP protein expression was evaluated by immunostaining and immunometric tests. Myocardial histology was performed, confirming no alteration of organ structure. While ANP and BNP are cardiac peptides, CNP is mainly an endothelial hormone; thus its expression, as well as that of NPR-B and NPR-C, was also evaluated in kidney and lung of an animal subgroup (n = 20). In heart, lower BNP mRNA levels in O vs CO (p = 0.02) as well as ANP and CNP (p = ns), were detected. NPR-B/NPR-A mRNA was similar in O and CO, while NPR-C was numerically lower (p = ns) in O than in CO. In kidney, CNP/NPR-B/NPR-C mRNA was similar in O and CO, while in lung CNP/NPR-C expression decreased and NPR-B increased (p = ns) in O vs CO. Subdividing into fasting and hyperglycemic rats, the pattern of mRNA expression for each gene analyzed remained unchanged. The trend observed in heart, kidney and lung for CNP protein concentrations and immunohistochemistry reflected the mRNA expression. TNF-α and IL-6 mRNA were measured in each tissue and no significant genotype effect was detected in any tissue. The main NP variations were observed at the cardiac level, suggesting a reduced release by cardiac cells. The understanding of mechanisms involved in the modulation of the NP system in obesity could be a useful starting point for future clinical study devoted to identifying new obesity treatment strategies.
format	article
author	Manuela Cabiati Serena Raucci Tiziana Liistro Eugenia Belcastro Tommaso Prescimone Chiara Caselli Marco Matteucci Patricia Iozzo Letizia Mattii Daniela Giannessi Silvia Del Ry
author_facet	Manuela Cabiati Serena Raucci Tiziana Liistro Eugenia Belcastro Tommaso Prescimone Chiara Caselli Marco Matteucci Patricia Iozzo Letizia Mattii Daniela Giannessi Silvia Del Ry
author_sort	Manuela Cabiati
title	Impact of obesity on the expression profile of natriuretic peptide system in a rat experimental model.
title_short	Impact of obesity on the expression profile of natriuretic peptide system in a rat experimental model.
title_full	Impact of obesity on the expression profile of natriuretic peptide system in a rat experimental model.
title_fullStr	Impact of obesity on the expression profile of natriuretic peptide system in a rat experimental model.
title_full_unstemmed	Impact of obesity on the expression profile of natriuretic peptide system in a rat experimental model.
title_sort	impact of obesity on the expression profile of natriuretic peptide system in a rat experimental model.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/263651c52d5f4319a3c5c52d36ba0591
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Impact of obesity on the expression profile of natriuretic peptide system in a rat experimental model.

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