STAT1/SOCS1/3 Are Involved in the Inflammation-Regulating Effect of GAS6/AXL in Periodontal Ligament Cells Induced by Porphyromonas gingivalis Lipopolysaccharide In Vitro

Periodontitis involves chronic inflammation of the tissues around the teeth caused by plaque and the corresponding immune response. Growth arrest-specific protein 6 (GAS6) and AXL receptor tyrosine kinase (AXL) are known to be involved in inflammatory diseases, while signal transducer and activator...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Shengnan Zhang, Yingjun Liu, Xuekui Wang, Na An, Xiangying Ouyang
Formato: article
Lenguaje:EN
Publicado: Hindawi Limited 2021
Materias:
Acceso en línea:https://doaj.org/article/26371158b7aa4920add00a163668d645
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:26371158b7aa4920add00a163668d645
record_format dspace
spelling oai:doaj.org-article:26371158b7aa4920add00a163668d6452021-11-08T02:37:11ZSTAT1/SOCS1/3 Are Involved in the Inflammation-Regulating Effect of GAS6/AXL in Periodontal Ligament Cells Induced by Porphyromonas gingivalis Lipopolysaccharide In Vitro2314-715610.1155/2021/9577695https://doaj.org/article/26371158b7aa4920add00a163668d6452021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/9577695https://doaj.org/toc/2314-7156Periodontitis involves chronic inflammation of the tissues around the teeth caused by plaque and the corresponding immune response. Growth arrest-specific protein 6 (GAS6) and AXL receptor tyrosine kinase (AXL) are known to be involved in inflammatory diseases, while signal transducer and activator of transcription-1 (STAT1) and suppressor of cytokine signaling (SOCS) are related to inflammatory processes. Moreover, miRNA34a directly targets AXL to regulate the AXL expression. However, the specific roles of GAS6 and AXL in periodontitis remain unclear. This study was designed to explore the effect and mechanism of AXL on the expression of inflammatory cytokines induced by Porphyromonas gingivalis lipopolysaccharide (P. gingivalis LPS) in human periodontal ligament cells (hPDLCs). The effects of different concentrations of P. gingivalis LPS on the expression of GAS6/AXL in hPDLCs were observed. Additionally, the effect of LPS on AXL was investigated by transfection of the miRNA34a inhibitor. AXL was knocked down or overexpressed to observe the release of inflammatory cytokines interleukin- (IL-) 8 and IL-6. The results showed that the expression levels of GAS6 and AXL decreased after P. gingivalis LPS infection. Transfection of a miR-34a inhibitor to hPDLCs demonstrated a role of miR-34a in the downregulation of AXL expression induced by LPS. Moreover, AXL knockdown or overexpression influencing the expression of IL-8 and IL-6 was investigated under LPS stimulation. AXL knockdown decreased the expression of STAT1 and SOCS1/3. Overall, these results demonstrate that AXL inhibits the expression of LPS-induced inflammatory cytokines in hPDLCs and that STAT1 and SOCS1/3 are involved in the regulation of inflammation by GAS6/AXL.Shengnan ZhangYingjun LiuXuekui WangNa AnXiangying OuyangHindawi LimitedarticleImmunologic diseases. AllergyRC581-607ENJournal of Immunology Research, Vol 2021 (2021)
institution DOAJ
collection DOAJ
language EN
topic Immunologic diseases. Allergy
RC581-607
spellingShingle Immunologic diseases. Allergy
RC581-607
Shengnan Zhang
Yingjun Liu
Xuekui Wang
Na An
Xiangying Ouyang
STAT1/SOCS1/3 Are Involved in the Inflammation-Regulating Effect of GAS6/AXL in Periodontal Ligament Cells Induced by Porphyromonas gingivalis Lipopolysaccharide In Vitro
description Periodontitis involves chronic inflammation of the tissues around the teeth caused by plaque and the corresponding immune response. Growth arrest-specific protein 6 (GAS6) and AXL receptor tyrosine kinase (AXL) are known to be involved in inflammatory diseases, while signal transducer and activator of transcription-1 (STAT1) and suppressor of cytokine signaling (SOCS) are related to inflammatory processes. Moreover, miRNA34a directly targets AXL to regulate the AXL expression. However, the specific roles of GAS6 and AXL in periodontitis remain unclear. This study was designed to explore the effect and mechanism of AXL on the expression of inflammatory cytokines induced by Porphyromonas gingivalis lipopolysaccharide (P. gingivalis LPS) in human periodontal ligament cells (hPDLCs). The effects of different concentrations of P. gingivalis LPS on the expression of GAS6/AXL in hPDLCs were observed. Additionally, the effect of LPS on AXL was investigated by transfection of the miRNA34a inhibitor. AXL was knocked down or overexpressed to observe the release of inflammatory cytokines interleukin- (IL-) 8 and IL-6. The results showed that the expression levels of GAS6 and AXL decreased after P. gingivalis LPS infection. Transfection of a miR-34a inhibitor to hPDLCs demonstrated a role of miR-34a in the downregulation of AXL expression induced by LPS. Moreover, AXL knockdown or overexpression influencing the expression of IL-8 and IL-6 was investigated under LPS stimulation. AXL knockdown decreased the expression of STAT1 and SOCS1/3. Overall, these results demonstrate that AXL inhibits the expression of LPS-induced inflammatory cytokines in hPDLCs and that STAT1 and SOCS1/3 are involved in the regulation of inflammation by GAS6/AXL.
format article
author Shengnan Zhang
Yingjun Liu
Xuekui Wang
Na An
Xiangying Ouyang
author_facet Shengnan Zhang
Yingjun Liu
Xuekui Wang
Na An
Xiangying Ouyang
author_sort Shengnan Zhang
title STAT1/SOCS1/3 Are Involved in the Inflammation-Regulating Effect of GAS6/AXL in Periodontal Ligament Cells Induced by Porphyromonas gingivalis Lipopolysaccharide In Vitro
title_short STAT1/SOCS1/3 Are Involved in the Inflammation-Regulating Effect of GAS6/AXL in Periodontal Ligament Cells Induced by Porphyromonas gingivalis Lipopolysaccharide In Vitro
title_full STAT1/SOCS1/3 Are Involved in the Inflammation-Regulating Effect of GAS6/AXL in Periodontal Ligament Cells Induced by Porphyromonas gingivalis Lipopolysaccharide In Vitro
title_fullStr STAT1/SOCS1/3 Are Involved in the Inflammation-Regulating Effect of GAS6/AXL in Periodontal Ligament Cells Induced by Porphyromonas gingivalis Lipopolysaccharide In Vitro
title_full_unstemmed STAT1/SOCS1/3 Are Involved in the Inflammation-Regulating Effect of GAS6/AXL in Periodontal Ligament Cells Induced by Porphyromonas gingivalis Lipopolysaccharide In Vitro
title_sort stat1/socs1/3 are involved in the inflammation-regulating effect of gas6/axl in periodontal ligament cells induced by porphyromonas gingivalis lipopolysaccharide in vitro
publisher Hindawi Limited
publishDate 2021
url https://doaj.org/article/26371158b7aa4920add00a163668d645
work_keys_str_mv AT shengnanzhang stat1socs13areinvolvedintheinflammationregulatingeffectofgas6axlinperiodontalligamentcellsinducedbyporphyromonasgingivalislipopolysaccharideinvitro
AT yingjunliu stat1socs13areinvolvedintheinflammationregulatingeffectofgas6axlinperiodontalligamentcellsinducedbyporphyromonasgingivalislipopolysaccharideinvitro
AT xuekuiwang stat1socs13areinvolvedintheinflammationregulatingeffectofgas6axlinperiodontalligamentcellsinducedbyporphyromonasgingivalislipopolysaccharideinvitro
AT naan stat1socs13areinvolvedintheinflammationregulatingeffectofgas6axlinperiodontalligamentcellsinducedbyporphyromonasgingivalislipopolysaccharideinvitro
AT xiangyingouyang stat1socs13areinvolvedintheinflammationregulatingeffectofgas6axlinperiodontalligamentcellsinducedbyporphyromonasgingivalislipopolysaccharideinvitro
_version_ 1718442982225477632