Synchronized resistive-pulse analysis with flow visualization for single micro- and nanoscale objects driven by optical vortex in double orifice

Abstract Resistive-pulse analysis is a powerful tool for identifying micro- and nanoscale objects. For low-concentration specimens, the pulse responses are rare, and it is difficult to obtain a sufficient number of electrical waveforms to clearly characterize the targets and reduce noise. In this st...

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Autores principales:	Kichitaro Nakajima, Ryoji Nakatsuka, Tetsuro Tsuji, Kentaro Doi, Satoyuki Kawano
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2645be167f4347be8f8e560754769c69
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spelling	oai:doaj.org-article:2645be167f4347be8f8e560754769c692021-12-02T14:53:42ZSynchronized resistive-pulse analysis with flow visualization for single micro- and nanoscale objects driven by optical vortex in double orifice10.1038/s41598-021-87822-72045-2322https://doaj.org/article/2645be167f4347be8f8e560754769c692021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87822-7https://doaj.org/toc/2045-2322Abstract Resistive-pulse analysis is a powerful tool for identifying micro- and nanoscale objects. For low-concentration specimens, the pulse responses are rare, and it is difficult to obtain a sufficient number of electrical waveforms to clearly characterize the targets and reduce noise. In this study, we conducted a periodic resistive-pulse analysis using an optical vortex and a double orifice, which repetitively senses a single micro- or nanoscale target particle with a diameter ranging from 700 nm to 2 $$\mu$$ μ m. The periodic motion results in the accumulation of a sufficient number of waveforms within a short period. Acquired pulses show periodic ionic-current drops associated with the translocation events through each orifice. Furthermore, a transparent fluidic device allows us to synchronously average the waveforms by the microscopic observation of the translocation events and improve the signal-to-noise ratio. By this method, we succeed in distinguishing single particle diameters. Additionally, the results of measured signals and the simultaneous high-speed observations are used to quantitatively and systematically discuss the effect of the complex fluid flow in the orifices on the amplitude of the resistive pulse. The synchronized resistive-pulse analysis by the optical vortex with the flow visualization improves the pulse-acquisition rate for a single specific particle and accuracy of the analysis, refining the micro- and nanoscale object identification.Kichitaro NakajimaRyoji NakatsukaTetsuro TsujiKentaro DoiSatoyuki KawanoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kichitaro Nakajima Ryoji Nakatsuka Tetsuro Tsuji Kentaro Doi Satoyuki Kawano Synchronized resistive-pulse analysis with flow visualization for single micro- and nanoscale objects driven by optical vortex in double orifice
description	Abstract Resistive-pulse analysis is a powerful tool for identifying micro- and nanoscale objects. For low-concentration specimens, the pulse responses are rare, and it is difficult to obtain a sufficient number of electrical waveforms to clearly characterize the targets and reduce noise. In this study, we conducted a periodic resistive-pulse analysis using an optical vortex and a double orifice, which repetitively senses a single micro- or nanoscale target particle with a diameter ranging from 700 nm to 2 $$\mu$$ μ m. The periodic motion results in the accumulation of a sufficient number of waveforms within a short period. Acquired pulses show periodic ionic-current drops associated with the translocation events through each orifice. Furthermore, a transparent fluidic device allows us to synchronously average the waveforms by the microscopic observation of the translocation events and improve the signal-to-noise ratio. By this method, we succeed in distinguishing single particle diameters. Additionally, the results of measured signals and the simultaneous high-speed observations are used to quantitatively and systematically discuss the effect of the complex fluid flow in the orifices on the amplitude of the resistive pulse. The synchronized resistive-pulse analysis by the optical vortex with the flow visualization improves the pulse-acquisition rate for a single specific particle and accuracy of the analysis, refining the micro- and nanoscale object identification.
format	article
author	Kichitaro Nakajima Ryoji Nakatsuka Tetsuro Tsuji Kentaro Doi Satoyuki Kawano
author_facet	Kichitaro Nakajima Ryoji Nakatsuka Tetsuro Tsuji Kentaro Doi Satoyuki Kawano
author_sort	Kichitaro Nakajima
title	Synchronized resistive-pulse analysis with flow visualization for single micro- and nanoscale objects driven by optical vortex in double orifice
title_short	Synchronized resistive-pulse analysis with flow visualization for single micro- and nanoscale objects driven by optical vortex in double orifice
title_full	Synchronized resistive-pulse analysis with flow visualization for single micro- and nanoscale objects driven by optical vortex in double orifice
title_fullStr	Synchronized resistive-pulse analysis with flow visualization for single micro- and nanoscale objects driven by optical vortex in double orifice
title_full_unstemmed	Synchronized resistive-pulse analysis with flow visualization for single micro- and nanoscale objects driven by optical vortex in double orifice
title_sort	synchronized resistive-pulse analysis with flow visualization for single micro- and nanoscale objects driven by optical vortex in double orifice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2645be167f4347be8f8e560754769c69
work_keys_str_mv	AT kichitaronakajima synchronizedresistivepulseanalysiswithflowvisualizationforsinglemicroandnanoscaleobjectsdrivenbyopticalvortexindoubleorifice AT ryojinakatsuka synchronizedresistivepulseanalysiswithflowvisualizationforsinglemicroandnanoscaleobjectsdrivenbyopticalvortexindoubleorifice AT tetsurotsuji synchronizedresistivepulseanalysiswithflowvisualizationforsinglemicroandnanoscaleobjectsdrivenbyopticalvortexindoubleorifice AT kentarodoi synchronizedresistivepulseanalysiswithflowvisualizationforsinglemicroandnanoscaleobjectsdrivenbyopticalvortexindoubleorifice AT satoyukikawano synchronizedresistivepulseanalysiswithflowvisualizationforsinglemicroandnanoscaleobjectsdrivenbyopticalvortexindoubleorifice
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Synchronized resistive-pulse analysis with flow visualization for single micro- and nanoscale objects driven by optical vortex in double orifice

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