Kochia scoparia seed extract suppresses VEGF-induced angiogenesis via modulating VEGF receptor 2 and PI3K/AKT/mTOR pathways

Context: Kochia scoparia (L.) Schrad (Amaranthaceae), known as a traditional medicine in China, Japan and Korea, is reported to have various biological activities. However, K. scoparia seed extract (KSE) functional roles on angiogenesis and prostate cancer inhibition have not been elucidated. Object...

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Autores principales:	Hyun-Dong Cho, Jeong-Ho Kim, Jun-Kyu Park, Seong-Min Hong, Du-Hyun Kim, Kwon-Il Seo
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2019
Materias:	herbal medicine antiangiogenesis anticancer vegf Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/264a51b7b3174f5cb3903723d1a53a30
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spelling	oai:doaj.org-article:264a51b7b3174f5cb3903723d1a53a302021-11-17T14:21:56ZKochia scoparia seed extract suppresses VEGF-induced angiogenesis via modulating VEGF receptor 2 and PI3K/AKT/mTOR pathways1388-02091744-511610.1080/13880209.2019.1672753https://doaj.org/article/264a51b7b3174f5cb3903723d1a53a302019-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2019.1672753https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context: Kochia scoparia (L.) Schrad (Amaranthaceae), known as a traditional medicine in China, Japan and Korea, is reported to have various biological activities. However, K. scoparia seed extract (KSE) functional roles on angiogenesis and prostate cancer inhibition have not been elucidated. Objective: This study elucidates the effects of KSE on vascular endothelial growth factor (VEGF)-induced angiogenesis in human umbilical vein endothelial cells (HUVECs) and inhibition of proliferation in prostate cancer cells. Materials and methods: HUVECs were treated with 10–20 µg/mL of KSE and 20–50 ng/mL of VEGF for 12–72 h. Anti-angiogenesis properties of KSE were determined by wound healing, trans-well, tube formation, rat aortic ring assay and western blotting. Prostate cancer and normal cells were incubated with 10–250 µg/mL of KSE for 24 h, and cell viability was measured by SRB assay. Phenolic compounds in KSE were analyzed using a HPLC-PDA system. Results: IC50 for cell viability of HUVECs, LNCaP, PC-3, RC-58T and RWPE-1 by KSE were 30.64, 89.25, 123.41, 141.62 and >250 µg/mL, respectively. Treatment with KSE (20 µg/mL) significantly suppressed VEGF-induced migration, invasion and capillary-like structure formation of HUVECs and microvessel sprouting from rat aortic rings. In addition, KSE down-regulated PI3K/AKT/mTOR levels and phosphorylation of VEGF receptor 2 in HUVECs. 3-OH-tyrosol (1.63 mg/g) and morin hydrate (0.17 mg/g) were identified in KSE. Conclusions: KSE inhibits angiogenesis in HUVECs as well as proliferation in human prostate cancer cells, suggesting KSE may be useful herbal medicine for preventing progression of prostate cancer and angiogenesis.Hyun-Dong ChoJeong-Ho KimJun-Kyu ParkSeong-Min HongDu-Hyun KimKwon-Il SeoTaylor & Francis Grouparticleherbal medicineantiangiogenesis anticancervegfTherapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 57, Iss 1, Pp 684-693 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	herbal medicine antiangiogenesis anticancer vegf Therapeutics. Pharmacology RM1-950
spellingShingle	herbal medicine antiangiogenesis anticancer vegf Therapeutics. Pharmacology RM1-950 Hyun-Dong Cho Jeong-Ho Kim Jun-Kyu Park Seong-Min Hong Du-Hyun Kim Kwon-Il Seo Kochia scoparia seed extract suppresses VEGF-induced angiogenesis via modulating VEGF receptor 2 and PI3K/AKT/mTOR pathways
description	Context: Kochia scoparia (L.) Schrad (Amaranthaceae), known as a traditional medicine in China, Japan and Korea, is reported to have various biological activities. However, K. scoparia seed extract (KSE) functional roles on angiogenesis and prostate cancer inhibition have not been elucidated. Objective: This study elucidates the effects of KSE on vascular endothelial growth factor (VEGF)-induced angiogenesis in human umbilical vein endothelial cells (HUVECs) and inhibition of proliferation in prostate cancer cells. Materials and methods: HUVECs were treated with 10–20 µg/mL of KSE and 20–50 ng/mL of VEGF for 12–72 h. Anti-angiogenesis properties of KSE were determined by wound healing, trans-well, tube formation, rat aortic ring assay and western blotting. Prostate cancer and normal cells were incubated with 10–250 µg/mL of KSE for 24 h, and cell viability was measured by SRB assay. Phenolic compounds in KSE were analyzed using a HPLC-PDA system. Results: IC50 for cell viability of HUVECs, LNCaP, PC-3, RC-58T and RWPE-1 by KSE were 30.64, 89.25, 123.41, 141.62 and >250 µg/mL, respectively. Treatment with KSE (20 µg/mL) significantly suppressed VEGF-induced migration, invasion and capillary-like structure formation of HUVECs and microvessel sprouting from rat aortic rings. In addition, KSE down-regulated PI3K/AKT/mTOR levels and phosphorylation of VEGF receptor 2 in HUVECs. 3-OH-tyrosol (1.63 mg/g) and morin hydrate (0.17 mg/g) were identified in KSE. Conclusions: KSE inhibits angiogenesis in HUVECs as well as proliferation in human prostate cancer cells, suggesting KSE may be useful herbal medicine for preventing progression of prostate cancer and angiogenesis.
format	article
author	Hyun-Dong Cho Jeong-Ho Kim Jun-Kyu Park Seong-Min Hong Du-Hyun Kim Kwon-Il Seo
author_facet	Hyun-Dong Cho Jeong-Ho Kim Jun-Kyu Park Seong-Min Hong Du-Hyun Kim Kwon-Il Seo
author_sort	Hyun-Dong Cho
title	Kochia scoparia seed extract suppresses VEGF-induced angiogenesis via modulating VEGF receptor 2 and PI3K/AKT/mTOR pathways
title_short	Kochia scoparia seed extract suppresses VEGF-induced angiogenesis via modulating VEGF receptor 2 and PI3K/AKT/mTOR pathways
title_full	Kochia scoparia seed extract suppresses VEGF-induced angiogenesis via modulating VEGF receptor 2 and PI3K/AKT/mTOR pathways
title_fullStr	Kochia scoparia seed extract suppresses VEGF-induced angiogenesis via modulating VEGF receptor 2 and PI3K/AKT/mTOR pathways
title_full_unstemmed	Kochia scoparia seed extract suppresses VEGF-induced angiogenesis via modulating VEGF receptor 2 and PI3K/AKT/mTOR pathways
title_sort	kochia scoparia seed extract suppresses vegf-induced angiogenesis via modulating vegf receptor 2 and pi3k/akt/mtor pathways
publisher	Taylor & Francis Group
publishDate	2019
url	https://doaj.org/article/264a51b7b3174f5cb3903723d1a53a30
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Kochia scoparia seed extract suppresses VEGF-induced angiogenesis via modulating VEGF receptor 2 and PI3K/AKT/mTOR pathways

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