The development of poly-L-arginine-coated liposomes for gene delivery

Praneet Opanasopit1, Jintana Tragulpakseerojn1, Auayporn Apirakaramwong1, Tanasait Ngawhirunpat1, Theerasak Rojanarata1, Uracha Ruktanonchai21Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, Thailand;&nbsp;2National Nanotechnology Center, Thailand Science Park, Pathumthani, Thailand...

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Autores principales:	Opanasopit P, Tragulpakseerojn J, Apirakaramwong A, Ngawhirunpat T, Rojanarata T, Ruktanonchai U
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/264b348ded6c4e62819ffbd923fd6982
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spelling	oai:doaj.org-article:264b348ded6c4e62819ffbd923fd69822021-12-02T02:52:42ZThe development of poly-L-arginine-coated liposomes for gene delivery1176-91141178-2013https://doaj.org/article/264b348ded6c4e62819ffbd923fd69822011-10-01T00:00:00Zhttp://www.dovepress.com/the-development-of-poly-l-arginine-coated-liposomes-for-gene-delivery-a8428https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Praneet Opanasopit1, Jintana Tragulpakseerojn1, Auayporn Apirakaramwong1, Tanasait Ngawhirunpat1, Theerasak Rojanarata1, Uracha Ruktanonchai21Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, Thailand;&nbsp;2National Nanotechnology Center, Thailand Science Park, Pathumthani, Thailand&nbsp;Abstract: In this study, liposomes coated with cationic polymers, poly-L-arginine (PLA), were assessed as a promising gene transfer system in human cervical carcinoma (HeLa) cells and human hepatoma cell line (Huh7) cells. The liposomes were prepared using egg yolk phosphatidylcholine and sodium oleate in the molar ratio of 10:2 with an ultrasonic generator and then coated with PLA. The PLA-coated liposomes (PCLs) formed complexes with plasmid DNA encoding green fluorescent protein. The complexes were characterized by agarose gel electrophoresis and investigated for their transfection efficiency in HeLa and Huh7 cells. The data were compared with PLA/DNA complexes and the positive control Lipofectamine 2000TM. The results showed that complete PCL/DNA complexes were formed at weight ratios of more than 0.05. Efficient gene transfer by PCLs was dependent on the cell type. The transfection efficiency of PCLs was about two times higher than that of PLA/DNA complexes in both HeLa cells and Huh7 cells. Cytotoxicity was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and showed that 80%-100% of both of the cells were viable after treating PCL/DNA complexes. The present results demonstrate that PCLs are a promising, nonviral gene carrier with low toxicity.Keywords: PLA-coated liposomes, PLA, gene delivery, transfection efficiency&nbsp;Opanasopit PTragulpakseerojn JApirakaramwong ANgawhirunpat TRojanarata TRuktanonchai UDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 2245-2252 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Opanasopit P Tragulpakseerojn J Apirakaramwong A Ngawhirunpat T Rojanarata T Ruktanonchai U The development of poly-L-arginine-coated liposomes for gene delivery
description	Praneet Opanasopit1, Jintana Tragulpakseerojn1, Auayporn Apirakaramwong1, Tanasait Ngawhirunpat1, Theerasak Rojanarata1, Uracha Ruktanonchai21Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, Thailand;&nbsp;2National Nanotechnology Center, Thailand Science Park, Pathumthani, Thailand&nbsp;Abstract: In this study, liposomes coated with cationic polymers, poly-L-arginine (PLA), were assessed as a promising gene transfer system in human cervical carcinoma (HeLa) cells and human hepatoma cell line (Huh7) cells. The liposomes were prepared using egg yolk phosphatidylcholine and sodium oleate in the molar ratio of 10:2 with an ultrasonic generator and then coated with PLA. The PLA-coated liposomes (PCLs) formed complexes with plasmid DNA encoding green fluorescent protein. The complexes were characterized by agarose gel electrophoresis and investigated for their transfection efficiency in HeLa and Huh7 cells. The data were compared with PLA/DNA complexes and the positive control Lipofectamine 2000TM. The results showed that complete PCL/DNA complexes were formed at weight ratios of more than 0.05. Efficient gene transfer by PCLs was dependent on the cell type. The transfection efficiency of PCLs was about two times higher than that of PLA/DNA complexes in both HeLa cells and Huh7 cells. Cytotoxicity was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and showed that 80%-100% of both of the cells were viable after treating PCL/DNA complexes. The present results demonstrate that PCLs are a promising, nonviral gene carrier with low toxicity.Keywords: PLA-coated liposomes, PLA, gene delivery, transfection efficiency&nbsp;
format	article
author	Opanasopit P Tragulpakseerojn J Apirakaramwong A Ngawhirunpat T Rojanarata T Ruktanonchai U
author_facet	Opanasopit P Tragulpakseerojn J Apirakaramwong A Ngawhirunpat T Rojanarata T Ruktanonchai U
author_sort	Opanasopit P
title	The development of poly-L-arginine-coated liposomes for gene delivery
title_short	The development of poly-L-arginine-coated liposomes for gene delivery
title_full	The development of poly-L-arginine-coated liposomes for gene delivery
title_fullStr	The development of poly-L-arginine-coated liposomes for gene delivery
title_full_unstemmed	The development of poly-L-arginine-coated liposomes for gene delivery
title_sort	development of poly-l-arginine-coated liposomes for gene delivery
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/264b348ded6c4e62819ffbd923fd6982
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The development of poly-L-arginine-coated liposomes for gene delivery

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