In vitro imaging of bacteria using 18F-fluorodeoxyglucose micro positron emission tomography

Abstract Positron emission tomography (PET) with fluorine-18-fluorodeoxyglucose (18F-FDG) can be applied to detect infection and inflammation. However, it was so far not known to what extent bacterial pathogens may contribute to the PET signal. Therefore, we investigated whether clinical isolates of...

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Autores principales:	Marjolein Heuker, Jürgen W. A. Sijbesma, Rocío Aguilar Suárez, Johan R. de Jong, Hendrikus H. Boersma, Gert Luurtsema, Philip H. Elsinga, Andor W. J. M. Glaudemans, Gooitzen M. van Dam, Jan Maarten van Dijl, Riemer H. J. A. Slart, Marleen van Oosten
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/264fc062fe304185807c54321efd1f2d
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spelling	oai:doaj.org-article:264fc062fe304185807c54321efd1f2d2021-12-02T16:06:21ZIn vitro imaging of bacteria using 18F-fluorodeoxyglucose micro positron emission tomography10.1038/s41598-017-05403-z2045-2322https://doaj.org/article/264fc062fe304185807c54321efd1f2d2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05403-zhttps://doaj.org/toc/2045-2322Abstract Positron emission tomography (PET) with fluorine-18-fluorodeoxyglucose (18F-FDG) can be applied to detect infection and inflammation. However, it was so far not known to what extent bacterial pathogens may contribute to the PET signal. Therefore, we investigated whether clinical isolates of frequently encountered bacterial pathogens take up 18F-FDG in vitro, and whether FDG inhibits bacterial growth as previously shown for 2-deoxy-glucose. 22 isolates of Gram-positive and Gram-negative bacterial pathogens implicated in fever and inflammation were incubated with 18F-FDG and uptake of 18F-FDG was assessed by gamma-counting and µPET imaging. Possible growth inhibition by FDG was assayed with Staphylococcus aureus and the Gram-positive model bacterium Bacillus subtilis. The results show that all tested isolates accumulated 18F-FDG actively. Further, 18F-FDG uptake was hampered in B. subtilis pts mutants impaired in glucose uptake. FDG inhibited growth of S. aureus and B. subtilis only to minor extents, and this effect was abrogated by pts mutations in B. subtilis. These observations imply that bacteria may contribute to the signals observed in FDG-PET infection imaging in vivo. Active bacterial FDG uptake is corroborated by the fact that the B. subtilis phosphotransferase system is needed for 18F-FDG uptake, while pts mutations protect against growth inhibition by FDG.Marjolein HeukerJürgen W. A. SijbesmaRocío Aguilar SuárezJohan R. de JongHendrikus H. BoersmaGert LuurtsemaPhilip H. ElsingaAndor W. J. M. GlaudemansGooitzen M. van DamJan Maarten van DijlRiemer H. J. A. SlartMarleen van OostenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marjolein Heuker Jürgen W. A. Sijbesma Rocío Aguilar Suárez Johan R. de Jong Hendrikus H. Boersma Gert Luurtsema Philip H. Elsinga Andor W. J. M. Glaudemans Gooitzen M. van Dam Jan Maarten van Dijl Riemer H. J. A. Slart Marleen van Oosten In vitro imaging of bacteria using 18F-fluorodeoxyglucose micro positron emission tomography
description	Abstract Positron emission tomography (PET) with fluorine-18-fluorodeoxyglucose (18F-FDG) can be applied to detect infection and inflammation. However, it was so far not known to what extent bacterial pathogens may contribute to the PET signal. Therefore, we investigated whether clinical isolates of frequently encountered bacterial pathogens take up 18F-FDG in vitro, and whether FDG inhibits bacterial growth as previously shown for 2-deoxy-glucose. 22 isolates of Gram-positive and Gram-negative bacterial pathogens implicated in fever and inflammation were incubated with 18F-FDG and uptake of 18F-FDG was assessed by gamma-counting and µPET imaging. Possible growth inhibition by FDG was assayed with Staphylococcus aureus and the Gram-positive model bacterium Bacillus subtilis. The results show that all tested isolates accumulated 18F-FDG actively. Further, 18F-FDG uptake was hampered in B. subtilis pts mutants impaired in glucose uptake. FDG inhibited growth of S. aureus and B. subtilis only to minor extents, and this effect was abrogated by pts mutations in B. subtilis. These observations imply that bacteria may contribute to the signals observed in FDG-PET infection imaging in vivo. Active bacterial FDG uptake is corroborated by the fact that the B. subtilis phosphotransferase system is needed for 18F-FDG uptake, while pts mutations protect against growth inhibition by FDG.
format	article
author	Marjolein Heuker Jürgen W. A. Sijbesma Rocío Aguilar Suárez Johan R. de Jong Hendrikus H. Boersma Gert Luurtsema Philip H. Elsinga Andor W. J. M. Glaudemans Gooitzen M. van Dam Jan Maarten van Dijl Riemer H. J. A. Slart Marleen van Oosten
author_facet	Marjolein Heuker Jürgen W. A. Sijbesma Rocío Aguilar Suárez Johan R. de Jong Hendrikus H. Boersma Gert Luurtsema Philip H. Elsinga Andor W. J. M. Glaudemans Gooitzen M. van Dam Jan Maarten van Dijl Riemer H. J. A. Slart Marleen van Oosten
author_sort	Marjolein Heuker
title	In vitro imaging of bacteria using 18F-fluorodeoxyglucose micro positron emission tomography
title_short	In vitro imaging of bacteria using 18F-fluorodeoxyglucose micro positron emission tomography
title_full	In vitro imaging of bacteria using 18F-fluorodeoxyglucose micro positron emission tomography
title_fullStr	In vitro imaging of bacteria using 18F-fluorodeoxyglucose micro positron emission tomography
title_full_unstemmed	In vitro imaging of bacteria using 18F-fluorodeoxyglucose micro positron emission tomography
title_sort	in vitro imaging of bacteria using 18f-fluorodeoxyglucose micro positron emission tomography
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/264fc062fe304185807c54321efd1f2d
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In vitro imaging of bacteria using 18F-fluorodeoxyglucose micro positron emission tomography

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