Tocotrienol-adjuvanted dendritic cells inhibit tumor growth and metastasis: a murine model of breast cancer.

Tocotrienol-rich fraction (TRF) from palm oil is reported to possess anti-cancer and immune-enhancing effects. In this study, TRF supplementation was used as an adjuvant to enhance the anti-cancer effects of dendritic cells (DC)-based cancer vaccine in a syngeneic mouse model of breast cancer. Femal...

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Autores principales:	Sitti Rahma Abdul Hafid, Srikumar Chakravarthi, Kalanithi Nesaretnam, Ammu Kutty Radhakrishnan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/26585ea126cb43b896d6d6885f1723f9
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spelling	oai:doaj.org-article:26585ea126cb43b896d6d6885f1723f92021-11-18T08:54:33ZTocotrienol-adjuvanted dendritic cells inhibit tumor growth and metastasis: a murine model of breast cancer.1932-620310.1371/journal.pone.0074753https://doaj.org/article/26585ea126cb43b896d6d6885f1723f92013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24069344/?tool=EBIhttps://doaj.org/toc/1932-6203Tocotrienol-rich fraction (TRF) from palm oil is reported to possess anti-cancer and immune-enhancing effects. In this study, TRF supplementation was used as an adjuvant to enhance the anti-cancer effects of dendritic cells (DC)-based cancer vaccine in a syngeneic mouse model of breast cancer. Female BALB/c mice were inoculated with 4T1 cells in mammary pad to induce tumor. When the tumor was palpable, the mice in the experimental groups were injected subcutaneously with DC-pulsed with tumor lysate (TL) from 4T1 cells (DC+TL) once a week for three weeks and fed daily with 1 mg TRF or vehicle. Control mice received unpulsed DC and were fed with vehicle. The combined therapy of using DC+TL injections and TRF supplementation (DC+TL+TRF) inhibited (p<0.05) tumor growth and metastasis. Splenocytes from the DC+TL+TRF group cultured with mitomycin-C (MMC)-treated 4T1 cells produced higher (p<0.05) levels of IFN-γ and IL-12. The cytotoxic T-lymphocyte (CTL) assay also showed enhanced tumor-specific killing (p<0.05) by CD8(+) T-lymphocytes isolated from mice in the DC+TL+TRF group. This study shows that TRF has the potential to be used as an adjuvant to enhance effectiveness of DC-based vaccines.Sitti Rahma Abdul HafidSrikumar ChakravarthiKalanithi NesaretnamAmmu Kutty RadhakrishnanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e74753 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sitti Rahma Abdul Hafid Srikumar Chakravarthi Kalanithi Nesaretnam Ammu Kutty Radhakrishnan Tocotrienol-adjuvanted dendritic cells inhibit tumor growth and metastasis: a murine model of breast cancer.
description	Tocotrienol-rich fraction (TRF) from palm oil is reported to possess anti-cancer and immune-enhancing effects. In this study, TRF supplementation was used as an adjuvant to enhance the anti-cancer effects of dendritic cells (DC)-based cancer vaccine in a syngeneic mouse model of breast cancer. Female BALB/c mice were inoculated with 4T1 cells in mammary pad to induce tumor. When the tumor was palpable, the mice in the experimental groups were injected subcutaneously with DC-pulsed with tumor lysate (TL) from 4T1 cells (DC+TL) once a week for three weeks and fed daily with 1 mg TRF or vehicle. Control mice received unpulsed DC and were fed with vehicle. The combined therapy of using DC+TL injections and TRF supplementation (DC+TL+TRF) inhibited (p<0.05) tumor growth and metastasis. Splenocytes from the DC+TL+TRF group cultured with mitomycin-C (MMC)-treated 4T1 cells produced higher (p<0.05) levels of IFN-γ and IL-12. The cytotoxic T-lymphocyte (CTL) assay also showed enhanced tumor-specific killing (p<0.05) by CD8(+) T-lymphocytes isolated from mice in the DC+TL+TRF group. This study shows that TRF has the potential to be used as an adjuvant to enhance effectiveness of DC-based vaccines.
format	article
author	Sitti Rahma Abdul Hafid Srikumar Chakravarthi Kalanithi Nesaretnam Ammu Kutty Radhakrishnan
author_facet	Sitti Rahma Abdul Hafid Srikumar Chakravarthi Kalanithi Nesaretnam Ammu Kutty Radhakrishnan
author_sort	Sitti Rahma Abdul Hafid
title	Tocotrienol-adjuvanted dendritic cells inhibit tumor growth and metastasis: a murine model of breast cancer.
title_short	Tocotrienol-adjuvanted dendritic cells inhibit tumor growth and metastasis: a murine model of breast cancer.
title_full	Tocotrienol-adjuvanted dendritic cells inhibit tumor growth and metastasis: a murine model of breast cancer.
title_fullStr	Tocotrienol-adjuvanted dendritic cells inhibit tumor growth and metastasis: a murine model of breast cancer.
title_full_unstemmed	Tocotrienol-adjuvanted dendritic cells inhibit tumor growth and metastasis: a murine model of breast cancer.
title_sort	tocotrienol-adjuvanted dendritic cells inhibit tumor growth and metastasis: a murine model of breast cancer.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/26585ea126cb43b896d6d6885f1723f9
work_keys_str_mv	AT sittirahmaabdulhafid tocotrienoladjuvanteddendriticcellsinhibittumorgrowthandmetastasisamurinemodelofbreastcancer AT srikumarchakravarthi tocotrienoladjuvanteddendriticcellsinhibittumorgrowthandmetastasisamurinemodelofbreastcancer AT kalanithinesaretnam tocotrienoladjuvanteddendriticcellsinhibittumorgrowthandmetastasisamurinemodelofbreastcancer AT ammukuttyradhakrishnan tocotrienoladjuvanteddendriticcellsinhibittumorgrowthandmetastasisamurinemodelofbreastcancer
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Tocotrienol-adjuvanted dendritic cells inhibit tumor growth and metastasis: a murine model of breast cancer.

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