Pterostilbene exerts anticancer activity on non-small-cell lung cancer via activating endoplasmic reticulum stress

Abstract Pterostilbene (PT), the natural dimethylated analog of resveratrol (RSV), is a potent anticarcinogen for non-small-cell lung cancer (NSCLC), but its anti-NSCLC mechanisms remain unclear. In this study, we show that PT treatment time- and dose-dependently enhanced the endoplasmic reticulum s...

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Autores principales:	Zhiqiang Ma, Yang Yang, Shouyin Di, Xiao Feng, Dong Liu, Shuai Jiang, Wei Hu, Zhigang Qin, Yue Li, Jianjun Lv, Chongxi Fan, Xiaolong Yan, Xiaofei Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2659c22ced554d26bcb696a3b90b514b
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spelling	oai:doaj.org-article:2659c22ced554d26bcb696a3b90b514b2021-12-02T16:08:24ZPterostilbene exerts anticancer activity on non-small-cell lung cancer via activating endoplasmic reticulum stress10.1038/s41598-017-08547-02045-2322https://doaj.org/article/2659c22ced554d26bcb696a3b90b514b2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08547-0https://doaj.org/toc/2045-2322Abstract Pterostilbene (PT), the natural dimethylated analog of resveratrol (RSV), is a potent anticarcinogen for non-small-cell lung cancer (NSCLC), but its anti-NSCLC mechanisms remain unclear. In this study, we show that PT treatment time- and dose-dependently enhanced the endoplasmic reticulum stress (ERS) signaling (i.e., p-PERK, IRE1, ATF4, CHOP), thus decreasing the cell viability and inducing apoptosis in human PC9 and A549 NSCLC cell lines. Moreover, the decreased migratory and adhesive abilities, downregulation of intracellular glutathione (GSH) level, enhanced reactive oxygen species (ROS) generation, Caspase 3 activity and mitochondrial membrane depolarization were observed in NSCLC cells treated with PT. These effects were reversed by CHOP siRNA which inhibited the ERS signaling pathway, but were promoted by thapsigargin (a classical ERS inducer) in vitro. Besides, in vivo studies also verify that PT exerted anticancer activity by mobilizing ERS signaling and apoptosis-related proteins, and these effects were enhanced by thapsigargin. Therefore, ERS activation may represent a new mechanism of anti-NSCLC action by PT, and a novel therapeutic intervention for lung cancer.Zhiqiang MaYang YangShouyin DiXiao FengDong LiuShuai JiangWei HuZhigang QinYue LiJianjun LvChongxi FanXiaolong YanXiaofei LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhiqiang Ma Yang Yang Shouyin Di Xiao Feng Dong Liu Shuai Jiang Wei Hu Zhigang Qin Yue Li Jianjun Lv Chongxi Fan Xiaolong Yan Xiaofei Li Pterostilbene exerts anticancer activity on non-small-cell lung cancer via activating endoplasmic reticulum stress
description	Abstract Pterostilbene (PT), the natural dimethylated analog of resveratrol (RSV), is a potent anticarcinogen for non-small-cell lung cancer (NSCLC), but its anti-NSCLC mechanisms remain unclear. In this study, we show that PT treatment time- and dose-dependently enhanced the endoplasmic reticulum stress (ERS) signaling (i.e., p-PERK, IRE1, ATF4, CHOP), thus decreasing the cell viability and inducing apoptosis in human PC9 and A549 NSCLC cell lines. Moreover, the decreased migratory and adhesive abilities, downregulation of intracellular glutathione (GSH) level, enhanced reactive oxygen species (ROS) generation, Caspase 3 activity and mitochondrial membrane depolarization were observed in NSCLC cells treated with PT. These effects were reversed by CHOP siRNA which inhibited the ERS signaling pathway, but were promoted by thapsigargin (a classical ERS inducer) in vitro. Besides, in vivo studies also verify that PT exerted anticancer activity by mobilizing ERS signaling and apoptosis-related proteins, and these effects were enhanced by thapsigargin. Therefore, ERS activation may represent a new mechanism of anti-NSCLC action by PT, and a novel therapeutic intervention for lung cancer.
format	article
author	Zhiqiang Ma Yang Yang Shouyin Di Xiao Feng Dong Liu Shuai Jiang Wei Hu Zhigang Qin Yue Li Jianjun Lv Chongxi Fan Xiaolong Yan Xiaofei Li
author_facet	Zhiqiang Ma Yang Yang Shouyin Di Xiao Feng Dong Liu Shuai Jiang Wei Hu Zhigang Qin Yue Li Jianjun Lv Chongxi Fan Xiaolong Yan Xiaofei Li
author_sort	Zhiqiang Ma
title	Pterostilbene exerts anticancer activity on non-small-cell lung cancer via activating endoplasmic reticulum stress
title_short	Pterostilbene exerts anticancer activity on non-small-cell lung cancer via activating endoplasmic reticulum stress
title_full	Pterostilbene exerts anticancer activity on non-small-cell lung cancer via activating endoplasmic reticulum stress
title_fullStr	Pterostilbene exerts anticancer activity on non-small-cell lung cancer via activating endoplasmic reticulum stress
title_full_unstemmed	Pterostilbene exerts anticancer activity on non-small-cell lung cancer via activating endoplasmic reticulum stress
title_sort	pterostilbene exerts anticancer activity on non-small-cell lung cancer via activating endoplasmic reticulum stress
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2659c22ced554d26bcb696a3b90b514b
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Pterostilbene exerts anticancer activity on non-small-cell lung cancer via activating endoplasmic reticulum stress

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