SARS-CoV-2 Prevalence in Laparoscopic Surgery Filters. Analysis in Patients with Negative Oropharyngeal RT-qPCR in a Pandemic Context: A Cross-Sectional Study

Objective: Surgical societies of different specialties have lately demonstrated a growing concern regarding the potential risk of SARS-CoV-2 transmission during surgery, mainly via aerosols carrying SARS-CoV-2 particles during laparoscopy smoke evacuation. Since there is not sufficient scientific ev...

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Autores principales:	Antoni Llueca, Manuela Barneo-Muñoz, Javier Escrig, Rosa de Llanos, on Behalf of COVID-Lap Working Group
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	SARS-CoV-2 laparoscopy smoke evacuation laparoscopy filters COVID-19 laparoscopic surgery Medicine R
Acceso en línea:	https://doaj.org/article/266a9c64d7724713b3c191b7360cab72
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spelling	oai:doaj.org-article:266a9c64d7724713b3c191b7360cab722021-11-25T18:06:45ZSARS-CoV-2 Prevalence in Laparoscopic Surgery Filters. Analysis in Patients with Negative Oropharyngeal RT-qPCR in a Pandemic Context: A Cross-Sectional Study10.3390/jpm111110522075-4426https://doaj.org/article/266a9c64d7724713b3c191b7360cab722021-10-01T00:00:00Zhttps://www.mdpi.com/2075-4426/11/11/1052https://doaj.org/toc/2075-4426Objective: Surgical societies of different specialties have lately demonstrated a growing concern regarding the potential risk of SARS-CoV-2 transmission during surgery, mainly via aerosols carrying SARS-CoV-2 particles during laparoscopy smoke evacuation. Since there is not sufficient scientific evidence to rule out this hypothesis, our study aimed to evaluate the prevalence of the appearance of SARS-CoV-2 genetic material in the in-filter membrane of the smoke filter systems, used in laparoscopic surgery, in a tertiary referral hospital during the peak phases of the pandemic. Methods: During the highest incidence of the pandemic outbreak, 180 laparoscopic smoke evacuation systems were collected from laparoscopies performed between April 2020 and May 2021 in University General Hospital of Castellón. As part of the safety protocol established as a result of the pandemic, an oropharyngeal reverse-transcription polymerase chain reaction (RT-PCR) was performed before surgery. We performed RT-qPCR tests for the detection and quantification of SARS-CoV-2 genetic material in the in-filter membranes extracted from the smoke evacuation systems. Results: We found two RT-qPCR positive in-filters from a sample of 128 patients with SARS-CoV-2-negative results in their oropharyngeal RT-qPCR, i.e., 1.6% (95% CI: 0.5–5.5%). From this estimation, the predictive posterior probabilities of finding <i>n</i> cases of negative oropharyngeal COVID-19 patients with positive filters increases with the increasing number of surgeries performed. Conclusions: This cross-sectional study provides evidence suggesting that airborne transmission of SARS-CoV-2 particles from smoke evacuation of aerosols carrying viral particles during laparoscopy should not be ruled out.Antoni LluecaManuela Barneo-MuñozJavier EscrigRosa de Llanoson Behalf of COVID-Lap Working GroupMDPI AGarticleSARS-CoV-2laparoscopy smoke evacuationlaparoscopy filtersCOVID-19laparoscopic surgeryMedicineRENJournal of Personalized Medicine, Vol 11, Iss 1052, p 1052 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	SARS-CoV-2 laparoscopy smoke evacuation laparoscopy filters COVID-19 laparoscopic surgery Medicine R
spellingShingle	SARS-CoV-2 laparoscopy smoke evacuation laparoscopy filters COVID-19 laparoscopic surgery Medicine R Antoni Llueca Manuela Barneo-Muñoz Javier Escrig Rosa de Llanos on Behalf of COVID-Lap Working Group SARS-CoV-2 Prevalence in Laparoscopic Surgery Filters. Analysis in Patients with Negative Oropharyngeal RT-qPCR in a Pandemic Context: A Cross-Sectional Study
description	Objective: Surgical societies of different specialties have lately demonstrated a growing concern regarding the potential risk of SARS-CoV-2 transmission during surgery, mainly via aerosols carrying SARS-CoV-2 particles during laparoscopy smoke evacuation. Since there is not sufficient scientific evidence to rule out this hypothesis, our study aimed to evaluate the prevalence of the appearance of SARS-CoV-2 genetic material in the in-filter membrane of the smoke filter systems, used in laparoscopic surgery, in a tertiary referral hospital during the peak phases of the pandemic. Methods: During the highest incidence of the pandemic outbreak, 180 laparoscopic smoke evacuation systems were collected from laparoscopies performed between April 2020 and May 2021 in University General Hospital of Castellón. As part of the safety protocol established as a result of the pandemic, an oropharyngeal reverse-transcription polymerase chain reaction (RT-PCR) was performed before surgery. We performed RT-qPCR tests for the detection and quantification of SARS-CoV-2 genetic material in the in-filter membranes extracted from the smoke evacuation systems. Results: We found two RT-qPCR positive in-filters from a sample of 128 patients with SARS-CoV-2-negative results in their oropharyngeal RT-qPCR, i.e., 1.6% (95% CI: 0.5–5.5%). From this estimation, the predictive posterior probabilities of finding <i>n</i> cases of negative oropharyngeal COVID-19 patients with positive filters increases with the increasing number of surgeries performed. Conclusions: This cross-sectional study provides evidence suggesting that airborne transmission of SARS-CoV-2 particles from smoke evacuation of aerosols carrying viral particles during laparoscopy should not be ruled out.
format	article
author	Antoni Llueca Manuela Barneo-Muñoz Javier Escrig Rosa de Llanos on Behalf of COVID-Lap Working Group
author_facet	Antoni Llueca Manuela Barneo-Muñoz Javier Escrig Rosa de Llanos on Behalf of COVID-Lap Working Group
author_sort	Antoni Llueca
title	SARS-CoV-2 Prevalence in Laparoscopic Surgery Filters. Analysis in Patients with Negative Oropharyngeal RT-qPCR in a Pandemic Context: A Cross-Sectional Study
title_short	SARS-CoV-2 Prevalence in Laparoscopic Surgery Filters. Analysis in Patients with Negative Oropharyngeal RT-qPCR in a Pandemic Context: A Cross-Sectional Study
title_full	SARS-CoV-2 Prevalence in Laparoscopic Surgery Filters. Analysis in Patients with Negative Oropharyngeal RT-qPCR in a Pandemic Context: A Cross-Sectional Study
title_fullStr	SARS-CoV-2 Prevalence in Laparoscopic Surgery Filters. Analysis in Patients with Negative Oropharyngeal RT-qPCR in a Pandemic Context: A Cross-Sectional Study
title_full_unstemmed	SARS-CoV-2 Prevalence in Laparoscopic Surgery Filters. Analysis in Patients with Negative Oropharyngeal RT-qPCR in a Pandemic Context: A Cross-Sectional Study
title_sort	sars-cov-2 prevalence in laparoscopic surgery filters. analysis in patients with negative oropharyngeal rt-qpcr in a pandemic context: a cross-sectional study
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/266a9c64d7724713b3c191b7360cab72
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SARS-CoV-2 Prevalence in Laparoscopic Surgery Filters. Analysis in Patients with Negative Oropharyngeal RT-qPCR in a Pandemic Context: A Cross-Sectional Study

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