Hepatic mitochondrial function analysis using needle liver biopsy samples.

<h4>Backgrounds and aim</h4>Current assessment of pre-operative liver function relies upon biochemical blood tests and histology but these only indirectly measure liver function. Mitochondrial function (MF) analysis allows direct measurement of cellular metabolic function and may provide...

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Autores principales:	Michael J J Chu, Anthony R J Phillips, Alexander W G Hosking, Julia R MacDonald, Adam S J R Bartlett, Anthony J R Hickey
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/266fe634e9b643ec8e043acff4dcb4f7
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spelling	oai:doaj.org-article:266fe634e9b643ec8e043acff4dcb4f72021-11-18T08:49:05ZHepatic mitochondrial function analysis using needle liver biopsy samples.1932-620310.1371/journal.pone.0079097https://doaj.org/article/266fe634e9b643ec8e043acff4dcb4f72013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24205366/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Backgrounds and aim</h4>Current assessment of pre-operative liver function relies upon biochemical blood tests and histology but these only indirectly measure liver function. Mitochondrial function (MF) analysis allows direct measurement of cellular metabolic function and may provide an additional index of hepatic health. Conventional MF analysis requires substantial tissue samples (>100 mg) obtained at open surgery. Here we report a method to assess MF using <3 mg of tissue obtained by a Tru-cut® biopsy needle making it suitable for percutaneous application.<h4>Methods</h4>An 18G Bard® Max-core® biopsy instrument was used to collect samples. The optimal Tru-cut® sample weight, stability in ice-cold University of Wisconsin solution, reproducibility and protocol utility was initially evaluated in Wistar rat livers then confirmed in human samples. MF was measured in saponin-permeabilized samples using high-resolution respirometry.<h4>Results</h4>The average mass of a single rat and human liver Tru-cut® biopsy was 5.60±0.30 and 5.16±0.15 mg, respectively (mean; standard error of mean). Two milligram of sample was found the lowest feasible mass for the MF assay. Tissue MF declined after 1 hour of cold storage. Six replicate measurements within rats and humans (n = 6 each) showed low coefficient of variation (<10%) in measurements of State-III respiration, electron transport chain (ETC) capacity and respiratory control ratio (RCR). Ischemic rat and human liver samples consistently showed lower State-III respiration, ETC capacity and RCR, compared to normal perfused liver samples.<h4>Conclusion</h4>Consistent measurement of liver MF and detection of derangement in a disease state was successfully demonstrated using less than half the tissue from a single Tru-cut® biopsy. Using this technique outpatient assessment of liver MF is now feasible, providing a new assay for the evaluation of hepatic function.Michael J J ChuAnthony R J PhillipsAlexander W G HoskingJulia R MacDonaldAdam S J R BartlettAnthony J R HickeyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 10, p e79097 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Michael J J Chu Anthony R J Phillips Alexander W G Hosking Julia R MacDonald Adam S J R Bartlett Anthony J R Hickey Hepatic mitochondrial function analysis using needle liver biopsy samples.
description	<h4>Backgrounds and aim</h4>Current assessment of pre-operative liver function relies upon biochemical blood tests and histology but these only indirectly measure liver function. Mitochondrial function (MF) analysis allows direct measurement of cellular metabolic function and may provide an additional index of hepatic health. Conventional MF analysis requires substantial tissue samples (>100 mg) obtained at open surgery. Here we report a method to assess MF using <3 mg of tissue obtained by a Tru-cut® biopsy needle making it suitable for percutaneous application.<h4>Methods</h4>An 18G Bard® Max-core® biopsy instrument was used to collect samples. The optimal Tru-cut® sample weight, stability in ice-cold University of Wisconsin solution, reproducibility and protocol utility was initially evaluated in Wistar rat livers then confirmed in human samples. MF was measured in saponin-permeabilized samples using high-resolution respirometry.<h4>Results</h4>The average mass of a single rat and human liver Tru-cut® biopsy was 5.60±0.30 and 5.16±0.15 mg, respectively (mean; standard error of mean). Two milligram of sample was found the lowest feasible mass for the MF assay. Tissue MF declined after 1 hour of cold storage. Six replicate measurements within rats and humans (n = 6 each) showed low coefficient of variation (<10%) in measurements of State-III respiration, electron transport chain (ETC) capacity and respiratory control ratio (RCR). Ischemic rat and human liver samples consistently showed lower State-III respiration, ETC capacity and RCR, compared to normal perfused liver samples.<h4>Conclusion</h4>Consistent measurement of liver MF and detection of derangement in a disease state was successfully demonstrated using less than half the tissue from a single Tru-cut® biopsy. Using this technique outpatient assessment of liver MF is now feasible, providing a new assay for the evaluation of hepatic function.
format	article
author	Michael J J Chu Anthony R J Phillips Alexander W G Hosking Julia R MacDonald Adam S J R Bartlett Anthony J R Hickey
author_facet	Michael J J Chu Anthony R J Phillips Alexander W G Hosking Julia R MacDonald Adam S J R Bartlett Anthony J R Hickey
author_sort	Michael J J Chu
title	Hepatic mitochondrial function analysis using needle liver biopsy samples.
title_short	Hepatic mitochondrial function analysis using needle liver biopsy samples.
title_full	Hepatic mitochondrial function analysis using needle liver biopsy samples.
title_fullStr	Hepatic mitochondrial function analysis using needle liver biopsy samples.
title_full_unstemmed	Hepatic mitochondrial function analysis using needle liver biopsy samples.
title_sort	hepatic mitochondrial function analysis using needle liver biopsy samples.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/266fe634e9b643ec8e043acff4dcb4f7
work_keys_str_mv	AT michaeljjchu hepaticmitochondrialfunctionanalysisusingneedleliverbiopsysamples AT anthonyrjphillips hepaticmitochondrialfunctionanalysisusingneedleliverbiopsysamples AT alexanderwghosking hepaticmitochondrialfunctionanalysisusingneedleliverbiopsysamples AT juliarmacdonald hepaticmitochondrialfunctionanalysisusingneedleliverbiopsysamples AT adamsjrbartlett hepaticmitochondrialfunctionanalysisusingneedleliverbiopsysamples AT anthonyjrhickey hepaticmitochondrialfunctionanalysisusingneedleliverbiopsysamples
_version_	1718421250375680000

Hepatic mitochondrial function analysis using needle liver biopsy samples.

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