A niosome formulation modulates the Th1/Th2 bias immune response in mice and also provides protection against anthrax spore challenge

Himanshu Gogoi, Rajesh Mani, Rakesh Bhatnagar Laboratory of Molecular Biology and Genetic Engineering, School of Biotechnology, Jawaharlal Nehru University, New Delhi, India Introduction: In this study, we have investigated the immunogenicity and protective efficacy of a niosomal formulation encap...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Gogoi H, Mani R, Bhatnagar R
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Bacillus anthracis niosome protective antigen protective antigen domain 4 Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/267108d481a24f6c9c65127a254a4b04
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:267108d481a24f6c9c65127a254a4b04
record_format	dspace
spelling	oai:doaj.org-article:267108d481a24f6c9c65127a254a4b042021-12-02T02:52:33ZA niosome formulation modulates the Th1/Th2 bias immune response in mice and also provides protection against anthrax spore challenge1178-2013https://doaj.org/article/267108d481a24f6c9c65127a254a4b042018-11-01T00:00:00Zhttps://www.dovepress.com/a-niosome-formulation-modulates-the-th1th2-bias-immune-response-in-mic-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Himanshu Gogoi, Rajesh Mani, Rakesh Bhatnagar Laboratory of Molecular Biology and Genetic Engineering, School of Biotechnology, Jawaharlal Nehru University, New Delhi, India Introduction: In this study, we have investigated the immunogenicity and protective efficacy of a niosomal formulation encapsulating protective antigen (PA) and PA domain 4 (D4) of Bacillus anthracis. Methods: Nonionic surfactant–based vesicles (NISV) + PA and NISV + D4 were prepared from span-60 and cholesterol by reverse-phase evaporation method and were evaluated for in vitro characteristics and immunological studies. Results: Particle characterization using transmission electron microscopy and atomic force microscopy analysis showed that the niosomal formulation was spherical in shape. The entrapment efficiency values were calculated to be 58.5% and 44.75% for PA and D4, respectively. Confocal microscopy and flow cytometry studies showed an enhanced uptake of antigen in THP1 macrophages by niosome as compared to antigen only. An in vitro release assay showed a burst release of antigen from niosome within 24 hours followed by a gradual release for 144 hours. Immunological studies showed that both PA- and D4-encapsulated niosome elicited a robust IgG titer. Antibody isotyping and cytokine profile showed that NISV + PA and NISV + D4 enhanced both Th1 and Th2 responses in mice, suggesting a mixed Th1/Th2 response. Both NISV + PA and NISV + D4 elicited high levels of anti-inflammatory cytokine interleukin-10 with low levels of pro-inflammatory cytokine tumor necrosis factor-α, suggesting the anti-inflammatory property of niosome. Both the niosomal formulations were also able to confer protection against BA infection as compared to only PA and D4. Conclusion: PA and D4 encapsulated NISV formulation could modulate both the Th1 and Th2 adaptive immune system and was found to be a better prophylactic against anthrax. Keywords: Bacillus anthracis, niosome, protective antigen, protective antigen domain 4Gogoi HMani RBhatnagar RDove Medical PressarticleBacillus anthracisniosomeprotective antigenprotective antigen domain 4Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 7427-7440 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bacillus anthracis niosome protective antigen protective antigen domain 4 Medicine (General) R5-920
spellingShingle	Bacillus anthracis niosome protective antigen protective antigen domain 4 Medicine (General) R5-920 Gogoi H Mani R Bhatnagar R A niosome formulation modulates the Th1/Th2 bias immune response in mice and also provides protection against anthrax spore challenge
description	Himanshu Gogoi, Rajesh Mani, Rakesh Bhatnagar Laboratory of Molecular Biology and Genetic Engineering, School of Biotechnology, Jawaharlal Nehru University, New Delhi, India Introduction: In this study, we have investigated the immunogenicity and protective efficacy of a niosomal formulation encapsulating protective antigen (PA) and PA domain 4 (D4) of Bacillus anthracis. Methods: Nonionic surfactant–based vesicles (NISV) + PA and NISV + D4 were prepared from span-60 and cholesterol by reverse-phase evaporation method and were evaluated for in vitro characteristics and immunological studies. Results: Particle characterization using transmission electron microscopy and atomic force microscopy analysis showed that the niosomal formulation was spherical in shape. The entrapment efficiency values were calculated to be 58.5% and 44.75% for PA and D4, respectively. Confocal microscopy and flow cytometry studies showed an enhanced uptake of antigen in THP1 macrophages by niosome as compared to antigen only. An in vitro release assay showed a burst release of antigen from niosome within 24 hours followed by a gradual release for 144 hours. Immunological studies showed that both PA- and D4-encapsulated niosome elicited a robust IgG titer. Antibody isotyping and cytokine profile showed that NISV + PA and NISV + D4 enhanced both Th1 and Th2 responses in mice, suggesting a mixed Th1/Th2 response. Both NISV + PA and NISV + D4 elicited high levels of anti-inflammatory cytokine interleukin-10 with low levels of pro-inflammatory cytokine tumor necrosis factor-α, suggesting the anti-inflammatory property of niosome. Both the niosomal formulations were also able to confer protection against BA infection as compared to only PA and D4. Conclusion: PA and D4 encapsulated NISV formulation could modulate both the Th1 and Th2 adaptive immune system and was found to be a better prophylactic against anthrax. Keywords: Bacillus anthracis, niosome, protective antigen, protective antigen domain 4
format	article
author	Gogoi H Mani R Bhatnagar R
author_facet	Gogoi H Mani R Bhatnagar R
author_sort	Gogoi H
title	A niosome formulation modulates the Th1/Th2 bias immune response in mice and also provides protection against anthrax spore challenge
title_short	A niosome formulation modulates the Th1/Th2 bias immune response in mice and also provides protection against anthrax spore challenge
title_full	A niosome formulation modulates the Th1/Th2 bias immune response in mice and also provides protection against anthrax spore challenge
title_fullStr	A niosome formulation modulates the Th1/Th2 bias immune response in mice and also provides protection against anthrax spore challenge
title_full_unstemmed	A niosome formulation modulates the Th1/Th2 bias immune response in mice and also provides protection against anthrax spore challenge
title_sort	niosome formulation modulates the th1/th2 bias immune response in mice and also provides protection against anthrax spore challenge
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/267108d481a24f6c9c65127a254a4b04
work_keys_str_mv	AT gogoih aniosomeformulationmodulatestheth1th2biasimmuneresponseinmiceandalsoprovidesprotectionagainstanthraxsporechallenge AT manir aniosomeformulationmodulatestheth1th2biasimmuneresponseinmiceandalsoprovidesprotectionagainstanthraxsporechallenge AT bhatnagarr aniosomeformulationmodulatestheth1th2biasimmuneresponseinmiceandalsoprovidesprotectionagainstanthraxsporechallenge AT gogoih niosomeformulationmodulatestheth1th2biasimmuneresponseinmiceandalsoprovidesprotectionagainstanthraxsporechallenge AT manir niosomeformulationmodulatestheth1th2biasimmuneresponseinmiceandalsoprovidesprotectionagainstanthraxsporechallenge AT bhatnagarr niosomeformulationmodulatestheth1th2biasimmuneresponseinmiceandalsoprovidesprotectionagainstanthraxsporechallenge
_version_	1718402090942857216

A niosome formulation modulates the Th1/Th2 bias immune response in mice and also provides protection against anthrax spore challenge

Ejemplares similares