A deletion affecting an LRR-RLK gene co-segregates with the fruit flat shape trait in peach

Abstract In peach, the flat phenotype is caused by a partially dominant allele in heterozygosis (Ss), fruits from homozygous trees (SS) abort a few weeks after fruit setting. Previous research has identified a SSR marker (UDP98–412) highly associated with the trait, found suitable for marker assiste...

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Autores principales:	Elena López-Girona, Yu Zhang, Iban Eduardo, José Ramón Hernández Mora, Konstantinos G. Alexiou, Pere Arús, María José Aranzana
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/26758a7bebb84311bc59c39193b5e910
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spelling	oai:doaj.org-article:26758a7bebb84311bc59c39193b5e9102021-12-02T11:41:21ZA deletion affecting an LRR-RLK gene co-segregates with the fruit flat shape trait in peach10.1038/s41598-017-07022-02045-2322https://doaj.org/article/26758a7bebb84311bc59c39193b5e9102017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07022-0https://doaj.org/toc/2045-2322Abstract In peach, the flat phenotype is caused by a partially dominant allele in heterozygosis (Ss), fruits from homozygous trees (SS) abort a few weeks after fruit setting. Previous research has identified a SSR marker (UDP98–412) highly associated with the trait, found suitable for marker assisted selection (MAS). Here we report a ∼10 Kb deletion affecting the gene PRUPE.6G281100, 400 Kb upstream of UDP98-412, co-segregating with the trait. This gene is a leucine-rich repeat receptor-like kinase (LRR-RLK) orthologous to the Brassinosteroid insensitive 1-associated receptor kinase 1 (BAK1) group. PCR markers suitable for MAS confirmed its strong association with the trait in a collection of 246 cultivars. They were used to evaluate the DNA from a round fruit derived from a somatic mutation of the flat variety ‘UFO-4’, revealing that the mutation affected the flat associated allele (S). Protein BLAST alignment identified significant hits with genes involved in different biological processes. Best protein hit occurred with AtRLP12, which may functionally complement CLAVATA2, a key regulator that controls the stem cell population size. RT-PCR analysis revealed the absence of transcription of the partially deleted allele. The data support PRUPE.6G281100 as a candidate gene for flat shape in peach.Elena López-GironaYu ZhangIban EduardoJosé Ramón Hernández MoraKonstantinos G. AlexiouPere ArúsMaría José AranzanaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elena López-Girona Yu Zhang Iban Eduardo José Ramón Hernández Mora Konstantinos G. Alexiou Pere Arús María José Aranzana A deletion affecting an LRR-RLK gene co-segregates with the fruit flat shape trait in peach
description	Abstract In peach, the flat phenotype is caused by a partially dominant allele in heterozygosis (Ss), fruits from homozygous trees (SS) abort a few weeks after fruit setting. Previous research has identified a SSR marker (UDP98–412) highly associated with the trait, found suitable for marker assisted selection (MAS). Here we report a ∼10 Kb deletion affecting the gene PRUPE.6G281100, 400 Kb upstream of UDP98-412, co-segregating with the trait. This gene is a leucine-rich repeat receptor-like kinase (LRR-RLK) orthologous to the Brassinosteroid insensitive 1-associated receptor kinase 1 (BAK1) group. PCR markers suitable for MAS confirmed its strong association with the trait in a collection of 246 cultivars. They were used to evaluate the DNA from a round fruit derived from a somatic mutation of the flat variety ‘UFO-4’, revealing that the mutation affected the flat associated allele (S). Protein BLAST alignment identified significant hits with genes involved in different biological processes. Best protein hit occurred with AtRLP12, which may functionally complement CLAVATA2, a key regulator that controls the stem cell population size. RT-PCR analysis revealed the absence of transcription of the partially deleted allele. The data support PRUPE.6G281100 as a candidate gene for flat shape in peach.
format	article
author	Elena López-Girona Yu Zhang Iban Eduardo José Ramón Hernández Mora Konstantinos G. Alexiou Pere Arús María José Aranzana
author_facet	Elena López-Girona Yu Zhang Iban Eduardo José Ramón Hernández Mora Konstantinos G. Alexiou Pere Arús María José Aranzana
author_sort	Elena López-Girona
title	A deletion affecting an LRR-RLK gene co-segregates with the fruit flat shape trait in peach
title_short	A deletion affecting an LRR-RLK gene co-segregates with the fruit flat shape trait in peach
title_full	A deletion affecting an LRR-RLK gene co-segregates with the fruit flat shape trait in peach
title_fullStr	A deletion affecting an LRR-RLK gene co-segregates with the fruit flat shape trait in peach
title_full_unstemmed	A deletion affecting an LRR-RLK gene co-segregates with the fruit flat shape trait in peach
title_sort	deletion affecting an lrr-rlk gene co-segregates with the fruit flat shape trait in peach
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/26758a7bebb84311bc59c39193b5e910
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A deletion affecting an LRR-RLK gene co-segregates with the fruit flat shape trait in peach

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