Enzyme and lateral flow monoclonal antibody-based immunoassays to simultaneously determine spirotetramat and spirotetramat-enol in foodstuffs

Abstract Spirotetramat is employed worldwide to fight insect pests due to its high efficiency. This chemical is quickly metabolized by plants into spirotetramat-enol, so current regulations establish that both compounds must be determined in foodstuffs for monitoring purposes. Nowadays, immunochemic...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ramón E. Cevallos-Cedeño, Consuelo Agulló, Antonio Abad-Fuentes, Antonio Abad-Somovilla, Josep V. Mercader
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/267f7dfce3fe4996a54109d7c95e3505
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:267f7dfce3fe4996a54109d7c95e3505
record_format	dspace
spelling	oai:doaj.org-article:267f7dfce3fe4996a54109d7c95e35052021-12-02T13:48:53ZEnzyme and lateral flow monoclonal antibody-based immunoassays to simultaneously determine spirotetramat and spirotetramat-enol in foodstuffs10.1038/s41598-021-81432-z2045-2322https://doaj.org/article/267f7dfce3fe4996a54109d7c95e35052021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81432-zhttps://doaj.org/toc/2045-2322Abstract Spirotetramat is employed worldwide to fight insect pests due to its high efficiency. This chemical is quickly metabolized by plants into spirotetramat-enol, so current regulations establish that both compounds must be determined in foodstuffs for monitoring purposes. Nowadays, immunochemical methods constitute rapid and cost-effective strategies for chemical contaminant analysis at trace levels. However, high-affinity binders and suitable bioconjugates are required. In this study, haptens with opposite functionalisation sites were synthesized in order to generate high-affinity monoclonal antibodies. A direct competitive enzyme-linked immunosorbent assay with an IC50 value for the sum of spirotetramat and spirotetramat-enol of 0.1 μg/L was developed using selected antibodies and a novel heterologous bioconjugate carrying a rationally-designed hapten. Studies with fortified grape, grape juice, and wine samples showed good precision and accuracy values, with limits of quantification well below the maximum residue limits. Excellent correlation of results was observed with a standard reference chromatographic method. As a step forward, a lateral flow immunoassay was developed for onsite screening analysis of spirotetramat in wine. This assay was successfully validated according to Regulation 519/2014/EU for semi-quantitative methods at concentrations in line with the legal levels of spirotetramat and spirotetramat-enol in grapes, with a satisfactory false suspect rate below 2%.Ramón E. Cevallos-CedeñoConsuelo AgullóAntonio Abad-FuentesAntonio Abad-SomovillaJosep V. MercaderNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ramón E. Cevallos-Cedeño Consuelo Agulló Antonio Abad-Fuentes Antonio Abad-Somovilla Josep V. Mercader Enzyme and lateral flow monoclonal antibody-based immunoassays to simultaneously determine spirotetramat and spirotetramat-enol in foodstuffs
description	Abstract Spirotetramat is employed worldwide to fight insect pests due to its high efficiency. This chemical is quickly metabolized by plants into spirotetramat-enol, so current regulations establish that both compounds must be determined in foodstuffs for monitoring purposes. Nowadays, immunochemical methods constitute rapid and cost-effective strategies for chemical contaminant analysis at trace levels. However, high-affinity binders and suitable bioconjugates are required. In this study, haptens with opposite functionalisation sites were synthesized in order to generate high-affinity monoclonal antibodies. A direct competitive enzyme-linked immunosorbent assay with an IC50 value for the sum of spirotetramat and spirotetramat-enol of 0.1 μg/L was developed using selected antibodies and a novel heterologous bioconjugate carrying a rationally-designed hapten. Studies with fortified grape, grape juice, and wine samples showed good precision and accuracy values, with limits of quantification well below the maximum residue limits. Excellent correlation of results was observed with a standard reference chromatographic method. As a step forward, a lateral flow immunoassay was developed for onsite screening analysis of spirotetramat in wine. This assay was successfully validated according to Regulation 519/2014/EU for semi-quantitative methods at concentrations in line with the legal levels of spirotetramat and spirotetramat-enol in grapes, with a satisfactory false suspect rate below 2%.
format	article
author	Ramón E. Cevallos-Cedeño Consuelo Agulló Antonio Abad-Fuentes Antonio Abad-Somovilla Josep V. Mercader
author_facet	Ramón E. Cevallos-Cedeño Consuelo Agulló Antonio Abad-Fuentes Antonio Abad-Somovilla Josep V. Mercader
author_sort	Ramón E. Cevallos-Cedeño
title	Enzyme and lateral flow monoclonal antibody-based immunoassays to simultaneously determine spirotetramat and spirotetramat-enol in foodstuffs
title_short	Enzyme and lateral flow monoclonal antibody-based immunoassays to simultaneously determine spirotetramat and spirotetramat-enol in foodstuffs
title_full	Enzyme and lateral flow monoclonal antibody-based immunoassays to simultaneously determine spirotetramat and spirotetramat-enol in foodstuffs
title_fullStr	Enzyme and lateral flow monoclonal antibody-based immunoassays to simultaneously determine spirotetramat and spirotetramat-enol in foodstuffs
title_full_unstemmed	Enzyme and lateral flow monoclonal antibody-based immunoassays to simultaneously determine spirotetramat and spirotetramat-enol in foodstuffs
title_sort	enzyme and lateral flow monoclonal antibody-based immunoassays to simultaneously determine spirotetramat and spirotetramat-enol in foodstuffs
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/267f7dfce3fe4996a54109d7c95e3505
work_keys_str_mv	AT ramonecevalloscedeno enzymeandlateralflowmonoclonalantibodybasedimmunoassaystosimultaneouslydeterminespirotetramatandspirotetramatenolinfoodstuffs AT consueloagullo enzymeandlateralflowmonoclonalantibodybasedimmunoassaystosimultaneouslydeterminespirotetramatandspirotetramatenolinfoodstuffs AT antonioabadfuentes enzymeandlateralflowmonoclonalantibodybasedimmunoassaystosimultaneouslydeterminespirotetramatandspirotetramatenolinfoodstuffs AT antonioabadsomovilla enzymeandlateralflowmonoclonalantibodybasedimmunoassaystosimultaneouslydeterminespirotetramatandspirotetramatenolinfoodstuffs AT josepvmercader enzymeandlateralflowmonoclonalantibodybasedimmunoassaystosimultaneouslydeterminespirotetramatandspirotetramatenolinfoodstuffs
_version_	1718392427692163072

Enzyme and lateral flow monoclonal antibody-based immunoassays to simultaneously determine spirotetramat and spirotetramat-enol in foodstuffs

Ejemplares similares