RNase 7 contributes to the cutaneous defense against Enterococcus faecium.

<h4>Background</h4>Human skin is able to mount a fast response against invading microorganisms by the release of antimicrobial proteins such as the ribonuclease RNase 7. Because RNase 7 exhibits high activity against Enterococcus faecium the aim of this study was to further explore the r...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Bente Köten, Maren Simanski, Regine Gläser, Rainer Podschun, Jens-Michael Schröder, Jürgen Harder
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2687647d37cf49b1a03fcd669b7c6af7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2687647d37cf49b1a03fcd669b7c6af7
record_format	dspace
spelling	oai:doaj.org-article:2687647d37cf49b1a03fcd669b7c6af72021-11-25T06:21:22ZRNase 7 contributes to the cutaneous defense against Enterococcus faecium.1932-620310.1371/journal.pone.0006424https://doaj.org/article/2687647d37cf49b1a03fcd669b7c6af72009-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19641608/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Human skin is able to mount a fast response against invading microorganisms by the release of antimicrobial proteins such as the ribonuclease RNase 7. Because RNase 7 exhibits high activity against Enterococcus faecium the aim of this study was to further explore the role of RNase 7 in the cutaneous innate defense system against E. faecium.<h4>Methodology/principal findings</h4>Absolute quantification using real-time PCR and ELISA revealed that primary keratinocytes expressed high levels of RNase 7. Immunohistochemistry showed RNase 7 expression in all epidermal layers of the skin with an intensification in the upper more differentiated layers. Furthermore, RNase 7 was secreted by keratinocytes in vitro and in vivo in a site-dependent way. RNase 7 was still active against E. faecium at low pH (5.5) or high NaCl (150 mM) concentration and the bactericidal activity of RNase 7 against E. faecium required no ribonuclease activity as shown by recombinant RNase 7 lacking enzymatic activity. To further explore the role of RNase 7 in cutaneous defense against E. faecium, we investigated whether RNase 7 contributes to the E. faecium killing activity of skin extracts derived from stratum corneum. Treatment of the skin extract with an RNase 7 specific antibody, which neutralizes the antimicrobial activity of RNase 7, diminished its E. faecium killing activity.<h4>Conclusions/significance</h4>Our data indicate that RNase 7 contributes to the E. faecium-killing activity of skin extracts and suggest an important role for RNase 7 in the protection of human skin against E. faecium colonization.Bente KötenMaren SimanskiRegine GläserRainer PodschunJens-Michael SchröderJürgen HarderPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 7, p e6424 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bente Köten Maren Simanski Regine Gläser Rainer Podschun Jens-Michael Schröder Jürgen Harder RNase 7 contributes to the cutaneous defense against Enterococcus faecium.
description	<h4>Background</h4>Human skin is able to mount a fast response against invading microorganisms by the release of antimicrobial proteins such as the ribonuclease RNase 7. Because RNase 7 exhibits high activity against Enterococcus faecium the aim of this study was to further explore the role of RNase 7 in the cutaneous innate defense system against E. faecium.<h4>Methodology/principal findings</h4>Absolute quantification using real-time PCR and ELISA revealed that primary keratinocytes expressed high levels of RNase 7. Immunohistochemistry showed RNase 7 expression in all epidermal layers of the skin with an intensification in the upper more differentiated layers. Furthermore, RNase 7 was secreted by keratinocytes in vitro and in vivo in a site-dependent way. RNase 7 was still active against E. faecium at low pH (5.5) or high NaCl (150 mM) concentration and the bactericidal activity of RNase 7 against E. faecium required no ribonuclease activity as shown by recombinant RNase 7 lacking enzymatic activity. To further explore the role of RNase 7 in cutaneous defense against E. faecium, we investigated whether RNase 7 contributes to the E. faecium killing activity of skin extracts derived from stratum corneum. Treatment of the skin extract with an RNase 7 specific antibody, which neutralizes the antimicrobial activity of RNase 7, diminished its E. faecium killing activity.<h4>Conclusions/significance</h4>Our data indicate that RNase 7 contributes to the E. faecium-killing activity of skin extracts and suggest an important role for RNase 7 in the protection of human skin against E. faecium colonization.
format	article
author	Bente Köten Maren Simanski Regine Gläser Rainer Podschun Jens-Michael Schröder Jürgen Harder
author_facet	Bente Köten Maren Simanski Regine Gläser Rainer Podschun Jens-Michael Schröder Jürgen Harder
author_sort	Bente Köten
title	RNase 7 contributes to the cutaneous defense against Enterococcus faecium.
title_short	RNase 7 contributes to the cutaneous defense against Enterococcus faecium.
title_full	RNase 7 contributes to the cutaneous defense against Enterococcus faecium.
title_fullStr	RNase 7 contributes to the cutaneous defense against Enterococcus faecium.
title_full_unstemmed	RNase 7 contributes to the cutaneous defense against Enterococcus faecium.
title_sort	rnase 7 contributes to the cutaneous defense against enterococcus faecium.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/2687647d37cf49b1a03fcd669b7c6af7
work_keys_str_mv	AT bentekoten rnase7contributestothecutaneousdefenseagainstenterococcusfaecium AT marensimanski rnase7contributestothecutaneousdefenseagainstenterococcusfaecium AT regineglaser rnase7contributestothecutaneousdefenseagainstenterococcusfaecium AT rainerpodschun rnase7contributestothecutaneousdefenseagainstenterococcusfaecium AT jensmichaelschroder rnase7contributestothecutaneousdefenseagainstenterococcusfaecium AT jurgenharder rnase7contributestothecutaneousdefenseagainstenterococcusfaecium
_version_	1718413793144340480

RNase 7 contributes to the cutaneous defense against Enterococcus faecium.

Ejemplares similares