PLD$ is involved in phagocytosis of microglia: expression and localization changes of PLD4 are correlated with activation state of microglia.

Phospholipase D4 (PLD4) is a recently identified protein that is mainly expressed in the ionized calcium binding adapter molecule 1 (Iba1)-positive microglia in the early postnatal mouse cerebellar white matter. Unlike PLD1 and PLD2, PLD4 exhibits no enzymatic activity for conversion of phosphatidyl...

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Autores principales: Yoshinori Otani, Yoshihide Yamaguchi, Yumi Sato, Teiichi Furuichi, Kazuhiro Ikenaka, Hiroshi Kitani, Hiroko Baba
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Publicado: Public Library of Science (PLoS) 2011
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Acceso en línea:https://doaj.org/article/26881169ee1f478b977a05c4a1387d66
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spelling oai:doaj.org-article:26881169ee1f478b977a05c4a1387d662021-11-18T07:34:18ZPLD$ is involved in phagocytosis of microglia: expression and localization changes of PLD4 are correlated with activation state of microglia.1932-620310.1371/journal.pone.0027544https://doaj.org/article/26881169ee1f478b977a05c4a1387d662011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22102906/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Phospholipase D4 (PLD4) is a recently identified protein that is mainly expressed in the ionized calcium binding adapter molecule 1 (Iba1)-positive microglia in the early postnatal mouse cerebellar white matter. Unlike PLD1 and PLD2, PLD4 exhibits no enzymatic activity for conversion of phosphatidylcholine into choline and phosphatidic acid, and its function is completely unknown. In the present study, we examined the distribution of PLD4 in mouse cerebellar white matter during development and under pathological conditions. Immunohistochemical analysis revealed that PLD4 expression was associated with microglial activation under such two different circumstances. A primary cultured microglia and microglial cell line (MG6) showed that PLD4 was mainly present in the nucleus, except the nucleolus, and expression of PLD4 was upregulated by lipopolysaccharide (LPS) stimulation. In the analysis of phagocytosis of LPS-stimulated microglia, PLD4 was co-localized with phagosomes that contained BioParticles. Inhibition of PLD4 expression using PLD4 specific small interfering RNA (siRNA) in MG6 cells significantly reduced the ratio of phagocytotic cell numbers. These results suggest that the increased PLD4 in the activation process is involved in phagocytosis of activated microglia in the developmental stages and pathological conditions of white matter.Yoshinori OtaniYoshihide YamaguchiYumi SatoTeiichi FuruichiKazuhiro IkenakaHiroshi KitaniHiroko BabaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 11, p e27544 (2011)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Yoshinori Otani
Yoshihide Yamaguchi
Yumi Sato
Teiichi Furuichi
Kazuhiro Ikenaka
Hiroshi Kitani
Hiroko Baba
PLD$ is involved in phagocytosis of microglia: expression and localization changes of PLD4 are correlated with activation state of microglia.
description Phospholipase D4 (PLD4) is a recently identified protein that is mainly expressed in the ionized calcium binding adapter molecule 1 (Iba1)-positive microglia in the early postnatal mouse cerebellar white matter. Unlike PLD1 and PLD2, PLD4 exhibits no enzymatic activity for conversion of phosphatidylcholine into choline and phosphatidic acid, and its function is completely unknown. In the present study, we examined the distribution of PLD4 in mouse cerebellar white matter during development and under pathological conditions. Immunohistochemical analysis revealed that PLD4 expression was associated with microglial activation under such two different circumstances. A primary cultured microglia and microglial cell line (MG6) showed that PLD4 was mainly present in the nucleus, except the nucleolus, and expression of PLD4 was upregulated by lipopolysaccharide (LPS) stimulation. In the analysis of phagocytosis of LPS-stimulated microglia, PLD4 was co-localized with phagosomes that contained BioParticles. Inhibition of PLD4 expression using PLD4 specific small interfering RNA (siRNA) in MG6 cells significantly reduced the ratio of phagocytotic cell numbers. These results suggest that the increased PLD4 in the activation process is involved in phagocytosis of activated microglia in the developmental stages and pathological conditions of white matter.
format article
author Yoshinori Otani
Yoshihide Yamaguchi
Yumi Sato
Teiichi Furuichi
Kazuhiro Ikenaka
Hiroshi Kitani
Hiroko Baba
author_facet Yoshinori Otani
Yoshihide Yamaguchi
Yumi Sato
Teiichi Furuichi
Kazuhiro Ikenaka
Hiroshi Kitani
Hiroko Baba
author_sort Yoshinori Otani
title PLD$ is involved in phagocytosis of microglia: expression and localization changes of PLD4 are correlated with activation state of microglia.
title_short PLD$ is involved in phagocytosis of microglia: expression and localization changes of PLD4 are correlated with activation state of microglia.
title_full PLD$ is involved in phagocytosis of microglia: expression and localization changes of PLD4 are correlated with activation state of microglia.
title_fullStr PLD$ is involved in phagocytosis of microglia: expression and localization changes of PLD4 are correlated with activation state of microglia.
title_full_unstemmed PLD$ is involved in phagocytosis of microglia: expression and localization changes of PLD4 are correlated with activation state of microglia.
title_sort pld$ is involved in phagocytosis of microglia: expression and localization changes of pld4 are correlated with activation state of microglia.
publisher Public Library of Science (PLoS)
publishDate 2011
url https://doaj.org/article/26881169ee1f478b977a05c4a1387d66
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