Colorimetric and Fluorescent Dual-Modality Sensing Platform Based on Fluorescent Nanozyme

Compared with natural enzymes, nanozymes based on carbonaceous nanomaterials are advantages due to high stability, good biocompatibility, and the possibility of multifunctionalities through materials engineering at an atomic level. Herein, we present a sensing platform using a nitrogen-doped graphen...

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Autores principales:	Yejian Wan, Jingwen Zhao, Xiaochun Deng, Jie Chen, Fengna Xi, Xiaobo Wang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	dual-modality sensing colorimetric detection fluorescent detection nanozyme graphene quantum dots Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/268dc2d7246a4d888e90ae0860c71558
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id	oai:doaj.org-article:268dc2d7246a4d888e90ae0860c71558
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spelling	oai:doaj.org-article:268dc2d7246a4d888e90ae0860c715582021-11-17T05:09:58ZColorimetric and Fluorescent Dual-Modality Sensing Platform Based on Fluorescent Nanozyme2296-264610.3389/fchem.2021.774486https://doaj.org/article/268dc2d7246a4d888e90ae0860c715582021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fchem.2021.774486/fullhttps://doaj.org/toc/2296-2646Compared with natural enzymes, nanozymes based on carbonaceous nanomaterials are advantages due to high stability, good biocompatibility, and the possibility of multifunctionalities through materials engineering at an atomic level. Herein, we present a sensing platform using a nitrogen-doped graphene quantum dot (NGQD) as a highly efficient fluorescent peroxidase mimic, which enables a colorimetric/fluorescent dual-modality platform for detection of hydrogen peroxide (H2O2) and biomolecules (ascorbic acid-AA, acid phosphatase-ACP) with high sensitivity. NGQD is synthesized using a simple hydrothermal process, which has advantages of high production yield and potential for large-scale preparation. NGQD with uniform size (3.0 ± 0.6 nm) and a single-layer graphene structure exhibits bright and stable fluorescence. N-doping and ultrasmall size endow NGQD with high peroxidase-mimicking activity with an obviously reduced Michaelis–Menten constant (Km) in comparison with natural horseradish peroxidase. Taking advantages of both high nanozyme activity and unique fluorescence property of NGQD, a colorimetric and fluorescent dual-modality platform capable of detecting H2O2 and biomolecules (AA, ACP) with high sensitivity is developed as the proof-of-concept demonstration. Furthermore, the mechanisms underlying the nanozyme activity and biosensing are investigated.Yejian WanJingwen ZhaoXiaochun DengJie ChenFengna XiXiaobo WangFrontiers Media S.A.articledual-modality sensingcolorimetric detectionfluorescent detectionnanozymegraphene quantum dotsChemistryQD1-999ENFrontiers in Chemistry, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dual-modality sensing colorimetric detection fluorescent detection nanozyme graphene quantum dots Chemistry QD1-999
spellingShingle	dual-modality sensing colorimetric detection fluorescent detection nanozyme graphene quantum dots Chemistry QD1-999 Yejian Wan Jingwen Zhao Xiaochun Deng Jie Chen Fengna Xi Xiaobo Wang Colorimetric and Fluorescent Dual-Modality Sensing Platform Based on Fluorescent Nanozyme
description	Compared with natural enzymes, nanozymes based on carbonaceous nanomaterials are advantages due to high stability, good biocompatibility, and the possibility of multifunctionalities through materials engineering at an atomic level. Herein, we present a sensing platform using a nitrogen-doped graphene quantum dot (NGQD) as a highly efficient fluorescent peroxidase mimic, which enables a colorimetric/fluorescent dual-modality platform for detection of hydrogen peroxide (H2O2) and biomolecules (ascorbic acid-AA, acid phosphatase-ACP) with high sensitivity. NGQD is synthesized using a simple hydrothermal process, which has advantages of high production yield and potential for large-scale preparation. NGQD with uniform size (3.0 ± 0.6 nm) and a single-layer graphene structure exhibits bright and stable fluorescence. N-doping and ultrasmall size endow NGQD with high peroxidase-mimicking activity with an obviously reduced Michaelis–Menten constant (Km) in comparison with natural horseradish peroxidase. Taking advantages of both high nanozyme activity and unique fluorescence property of NGQD, a colorimetric and fluorescent dual-modality platform capable of detecting H2O2 and biomolecules (AA, ACP) with high sensitivity is developed as the proof-of-concept demonstration. Furthermore, the mechanisms underlying the nanozyme activity and biosensing are investigated.
format	article
author	Yejian Wan Jingwen Zhao Xiaochun Deng Jie Chen Fengna Xi Xiaobo Wang
author_facet	Yejian Wan Jingwen Zhao Xiaochun Deng Jie Chen Fengna Xi Xiaobo Wang
author_sort	Yejian Wan
title	Colorimetric and Fluorescent Dual-Modality Sensing Platform Based on Fluorescent Nanozyme
title_short	Colorimetric and Fluorescent Dual-Modality Sensing Platform Based on Fluorescent Nanozyme
title_full	Colorimetric and Fluorescent Dual-Modality Sensing Platform Based on Fluorescent Nanozyme
title_fullStr	Colorimetric and Fluorescent Dual-Modality Sensing Platform Based on Fluorescent Nanozyme
title_full_unstemmed	Colorimetric and Fluorescent Dual-Modality Sensing Platform Based on Fluorescent Nanozyme
title_sort	colorimetric and fluorescent dual-modality sensing platform based on fluorescent nanozyme
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/268dc2d7246a4d888e90ae0860c71558
work_keys_str_mv	AT yejianwan colorimetricandfluorescentdualmodalitysensingplatformbasedonfluorescentnanozyme AT jingwenzhao colorimetricandfluorescentdualmodalitysensingplatformbasedonfluorescentnanozyme AT xiaochundeng colorimetricandfluorescentdualmodalitysensingplatformbasedonfluorescentnanozyme AT jiechen colorimetricandfluorescentdualmodalitysensingplatformbasedonfluorescentnanozyme AT fengnaxi colorimetricandfluorescentdualmodalitysensingplatformbasedonfluorescentnanozyme AT xiaobowang colorimetricandfluorescentdualmodalitysensingplatformbasedonfluorescentnanozyme
_version_	1718425913373229056

Colorimetric and Fluorescent Dual-Modality Sensing Platform Based on Fluorescent Nanozyme

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