Bicaudal is a conserved substrate for Drosophila and mammalian caspases and is essential for cell survival.

Members of the caspase family of cysteine proteases coordinate cell death through restricted proteolysis of diverse protein substrates and play a conserved role in apoptosis from nematodes to man. However, while numerous substrates for the mammalian cell death-associated caspases have now been descr...

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Autores principales:	Emma M Creagh, Gabriela Brumatti, Clare Sheridan, Patrick J Duriez, Rebecca C Taylor, Sean P Cullen, Colin Adrain, Seamus J Martin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2694c5ed2a8849ef8cf7796054f2aa09
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spelling	oai:doaj.org-article:2694c5ed2a8849ef8cf7796054f2aa092021-11-25T06:16:27ZBicaudal is a conserved substrate for Drosophila and mammalian caspases and is essential for cell survival.1932-620310.1371/journal.pone.0005055https://doaj.org/article/2694c5ed2a8849ef8cf7796054f2aa092009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19330035/?tool=EBIhttps://doaj.org/toc/1932-6203Members of the caspase family of cysteine proteases coordinate cell death through restricted proteolysis of diverse protein substrates and play a conserved role in apoptosis from nematodes to man. However, while numerous substrates for the mammalian cell death-associated caspases have now been described, few caspase substrates have been identified in other organisms. Here, we have utilized a proteomics-based approach to identify proteins that are cleaved by caspases during apoptosis in Drosophila D-Mel2 cells, a subline of the Schneider S2 cell line. This approach identified multiple novel substrates for the fly caspases and revealed that bicaudal/betaNAC is a conserved substrate for Drosophila and mammalian caspases. RNAi-mediated silencing of bicaudal expression in Drosophila D-Mel2 cells resulted in a block to proliferation, followed by spontaneous apoptosis. Similarly, silencing of expression of the mammalian bicaudal homologue, betaNAC, in HeLa, HEK293T, MCF-7 and MRC5 cells also resulted in spontaneous apoptosis. These data suggest that bicaudal/betaNAC is essential for cell survival and is a conserved target of caspases from flies to man.Emma M CreaghGabriela BrumattiClare SheridanPatrick J DuriezRebecca C TaylorSean P CullenColin AdrainSeamus J MartinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 3, p e5055 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Emma M Creagh Gabriela Brumatti Clare Sheridan Patrick J Duriez Rebecca C Taylor Sean P Cullen Colin Adrain Seamus J Martin Bicaudal is a conserved substrate for Drosophila and mammalian caspases and is essential for cell survival.
description	Members of the caspase family of cysteine proteases coordinate cell death through restricted proteolysis of diverse protein substrates and play a conserved role in apoptosis from nematodes to man. However, while numerous substrates for the mammalian cell death-associated caspases have now been described, few caspase substrates have been identified in other organisms. Here, we have utilized a proteomics-based approach to identify proteins that are cleaved by caspases during apoptosis in Drosophila D-Mel2 cells, a subline of the Schneider S2 cell line. This approach identified multiple novel substrates for the fly caspases and revealed that bicaudal/betaNAC is a conserved substrate for Drosophila and mammalian caspases. RNAi-mediated silencing of bicaudal expression in Drosophila D-Mel2 cells resulted in a block to proliferation, followed by spontaneous apoptosis. Similarly, silencing of expression of the mammalian bicaudal homologue, betaNAC, in HeLa, HEK293T, MCF-7 and MRC5 cells also resulted in spontaneous apoptosis. These data suggest that bicaudal/betaNAC is essential for cell survival and is a conserved target of caspases from flies to man.
format	article
author	Emma M Creagh Gabriela Brumatti Clare Sheridan Patrick J Duriez Rebecca C Taylor Sean P Cullen Colin Adrain Seamus J Martin
author_facet	Emma M Creagh Gabriela Brumatti Clare Sheridan Patrick J Duriez Rebecca C Taylor Sean P Cullen Colin Adrain Seamus J Martin
author_sort	Emma M Creagh
title	Bicaudal is a conserved substrate for Drosophila and mammalian caspases and is essential for cell survival.
title_short	Bicaudal is a conserved substrate for Drosophila and mammalian caspases and is essential for cell survival.
title_full	Bicaudal is a conserved substrate for Drosophila and mammalian caspases and is essential for cell survival.
title_fullStr	Bicaudal is a conserved substrate for Drosophila and mammalian caspases and is essential for cell survival.
title_full_unstemmed	Bicaudal is a conserved substrate for Drosophila and mammalian caspases and is essential for cell survival.
title_sort	bicaudal is a conserved substrate for drosophila and mammalian caspases and is essential for cell survival.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/2694c5ed2a8849ef8cf7796054f2aa09
work_keys_str_mv	AT emmamcreagh bicaudalisaconservedsubstratefordrosophilaandmammaliancaspasesandisessentialforcellsurvival AT gabrielabrumatti bicaudalisaconservedsubstratefordrosophilaandmammaliancaspasesandisessentialforcellsurvival AT claresheridan bicaudalisaconservedsubstratefordrosophilaandmammaliancaspasesandisessentialforcellsurvival AT patrickjduriez bicaudalisaconservedsubstratefordrosophilaandmammaliancaspasesandisessentialforcellsurvival AT rebeccactaylor bicaudalisaconservedsubstratefordrosophilaandmammaliancaspasesandisessentialforcellsurvival AT seanpcullen bicaudalisaconservedsubstratefordrosophilaandmammaliancaspasesandisessentialforcellsurvival AT colinadrain bicaudalisaconservedsubstratefordrosophilaandmammaliancaspasesandisessentialforcellsurvival AT seamusjmartin bicaudalisaconservedsubstratefordrosophilaandmammaliancaspasesandisessentialforcellsurvival
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Bicaudal is a conserved substrate for Drosophila and mammalian caspases and is essential for cell survival.

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