CHEMOKINE RECEPTORS AT DISTINCT DIFFERENTIATION STAGES OF T-HELPERS FROM PERIPHERAL BLOOD

Expression of chemokine receptors (CCR4, CCR6, CXCR3 and CXCR5) on T-helper (Th) cells at various levels of differentiation in a group of healthy volunteers (n = 52) was assessed on the basis of CD45RA and CD62L expression, using the eight-color flow cytometry. It was found that the “naive” T helper...

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Autores principales:	I. V. Kudryavtsev, A. G. Borisov, I. I. Krobinets, A. A. Savchenko, M. K. Serebriakova, Areg A. Totolian
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2016
Materias:	chemokine receptors t helper subsets ccr4 and cxcr5 ccr6 and cxcr3 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/26981136c2054b55858782ab3e0eb3b7
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spelling	oai:doaj.org-article:26981136c2054b55858782ab3e0eb3b72021-11-18T08:03:45ZCHEMOKINE RECEPTORS AT DISTINCT DIFFERENTIATION STAGES OF T-HELPERS FROM PERIPHERAL BLOOD1563-06252313-741X10.15789/1563-0625-2016-3-239-250https://doaj.org/article/26981136c2054b55858782ab3e0eb3b72016-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1036https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XExpression of chemokine receptors (CCR4, CCR6, CXCR3 and CXCR5) on T-helper (Th) cells at various levels of differentiation in a group of healthy volunteers (n = 52) was assessed on the basis of CD45RA and CD62L expression, using the eight-color flow cytometry. It was found that the “naive” T helper cells (N) with CD45RA+CD62L+ phenotype express CXCR3 (4.94±0.39%), and CXCR5 (3.63±0.25%). About 50% of central memory T helpers (CD45RA–CD62L+, CM) were CXCR3 positive, and 43.72±1.27% of CM cells expressed CCR6, whereas CXCR5 and CCR4 levels were about 30%. Furthermore, CXCR3 was expressed by 76.76±0.75% of the CD3+CD4+CD45RA–CD62L– (EM) population, and similar values were obtained for CCR6, while the relative abundance of CXCR5+ cells decreased to 13.68±0.50%, and CCR4 levels did not change and accounted for 33.26±1.13% positive cells. Likewise, co-expression of the chemokine receptors was studied for the abovementioned subpopulations of T helper cells. Among the CXCR5– Th, Th1 cells were identified as CXCR3+CCR6–CCR4– (this subset also contained Th9), and CXCR3+CCR6+CCR4– subsets, referred to as Th1/Th17. Th2 were detected on the basis of CCR4 expression in absence of all other chemokine receptors. In addition to the mentioned Th1/Th17 populations, Th 17 cells were found in the subsets of Th17 CXCR3–CCR6+CCR4– and CXCR3–CR6+CCR4+. The latter also contained a Th22 population. Follicular Th cell populations (CXCR5+) consisted of, at least, six different subsets: CXCR3–CCR6–CCR4– (Tfh/Tfh2), CXCR3–CCR6–CCR4+ (Tfh2), CXCR3-CCR6+CCR4–(Tfh17), CXCR3–CCR6+CCR4+ (Tfh17), CXCR3+CCR6–CCR4– (Tfh1) and CXCR3+CCR6+CCR4–(Tfh1/Tfh17). The cells with Th1/Th9 and Th1/Th17 phenotypes dominated among CM (about 13%), whereas their relative abundance within EM increased to 22.37±1.69% and 31.69±1.52%, respectively. The amounts of Th2 were 8.15±0.46% within CM, and only 1.72±0.15% for EM population. For the cells with Th17/Th22 phenotype, these values are 8.07±0.30% and 12.03±0.57%, respectively. The main Tfh subsets were represented among the CM T-helpers: the relative content of Tfh/Tfh2 was 5.79 0.26%, Tfh2, 1.34±0.07%; Tfh17 with CXCR3-CCR6+CCR4– and CXCR3-CCR6+CCR4+ phenotypes made up to 6.22±0.28% and 3.28±0.16%, as well as Tfh1 (7.68±0.31%), and Tfh1/Tfh17 (4.02±0.17%), respectively. Relative content of the mentioned Tfh subsets was decreased > 2-fold within effector memory Th subpopulation. The data obtained may be applied for diagnostics of different immunopathological conditions and could be used as a comparison group in further studies.I. V. KudryavtsevA. G. BorisovI. I. KrobinetsA. A. SavchenkoM. K. SerebriakovaAreg A. TotolianSPb RAACIarticlechemokine receptorst helper subsetsccr4 and cxcr5ccr6 and cxcr3Immunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 18, Iss 3, Pp 239-250 (2016)
institution	DOAJ
collection	DOAJ
language	RU
topic	chemokine receptors t helper subsets ccr4 and cxcr5 ccr6 and cxcr3 Immunologic diseases. Allergy RC581-607
spellingShingle	chemokine receptors t helper subsets ccr4 and cxcr5 ccr6 and cxcr3 Immunologic diseases. Allergy RC581-607 I. V. Kudryavtsev A. G. Borisov I. I. Krobinets A. A. Savchenko M. K. Serebriakova Areg A. Totolian CHEMOKINE RECEPTORS AT DISTINCT DIFFERENTIATION STAGES OF T-HELPERS FROM PERIPHERAL BLOOD
description	Expression of chemokine receptors (CCR4, CCR6, CXCR3 and CXCR5) on T-helper (Th) cells at various levels of differentiation in a group of healthy volunteers (n = 52) was assessed on the basis of CD45RA and CD62L expression, using the eight-color flow cytometry. It was found that the “naive” T helper cells (N) with CD45RA+CD62L+ phenotype express CXCR3 (4.94±0.39%), and CXCR5 (3.63±0.25%). About 50% of central memory T helpers (CD45RA–CD62L+, CM) were CXCR3 positive, and 43.72±1.27% of CM cells expressed CCR6, whereas CXCR5 and CCR4 levels were about 30%. Furthermore, CXCR3 was expressed by 76.76±0.75% of the CD3+CD4+CD45RA–CD62L– (EM) population, and similar values were obtained for CCR6, while the relative abundance of CXCR5+ cells decreased to 13.68±0.50%, and CCR4 levels did not change and accounted for 33.26±1.13% positive cells. Likewise, co-expression of the chemokine receptors was studied for the abovementioned subpopulations of T helper cells. Among the CXCR5– Th, Th1 cells were identified as CXCR3+CCR6–CCR4– (this subset also contained Th9), and CXCR3+CCR6+CCR4– subsets, referred to as Th1/Th17. Th2 were detected on the basis of CCR4 expression in absence of all other chemokine receptors. In addition to the mentioned Th1/Th17 populations, Th 17 cells were found in the subsets of Th17 CXCR3–CCR6+CCR4– and CXCR3–CR6+CCR4+. The latter also contained a Th22 population. Follicular Th cell populations (CXCR5+) consisted of, at least, six different subsets: CXCR3–CCR6–CCR4– (Tfh/Tfh2), CXCR3–CCR6–CCR4+ (Tfh2), CXCR3-CCR6+CCR4–(Tfh17), CXCR3–CCR6+CCR4+ (Tfh17), CXCR3+CCR6–CCR4– (Tfh1) and CXCR3+CCR6+CCR4–(Tfh1/Tfh17). The cells with Th1/Th9 and Th1/Th17 phenotypes dominated among CM (about 13%), whereas their relative abundance within EM increased to 22.37±1.69% and 31.69±1.52%, respectively. The amounts of Th2 were 8.15±0.46% within CM, and only 1.72±0.15% for EM population. For the cells with Th17/Th22 phenotype, these values are 8.07±0.30% and 12.03±0.57%, respectively. The main Tfh subsets were represented among the CM T-helpers: the relative content of Tfh/Tfh2 was 5.79 0.26%, Tfh2, 1.34±0.07%; Tfh17 with CXCR3-CCR6+CCR4– and CXCR3-CCR6+CCR4+ phenotypes made up to 6.22±0.28% and 3.28±0.16%, as well as Tfh1 (7.68±0.31%), and Tfh1/Tfh17 (4.02±0.17%), respectively. Relative content of the mentioned Tfh subsets was decreased > 2-fold within effector memory Th subpopulation. The data obtained may be applied for diagnostics of different immunopathological conditions and could be used as a comparison group in further studies.
format	article
author	I. V. Kudryavtsev A. G. Borisov I. I. Krobinets A. A. Savchenko M. K. Serebriakova Areg A. Totolian
author_facet	I. V. Kudryavtsev A. G. Borisov I. I. Krobinets A. A. Savchenko M. K. Serebriakova Areg A. Totolian
author_sort	I. V. Kudryavtsev
title	CHEMOKINE RECEPTORS AT DISTINCT DIFFERENTIATION STAGES OF T-HELPERS FROM PERIPHERAL BLOOD
title_short	CHEMOKINE RECEPTORS AT DISTINCT DIFFERENTIATION STAGES OF T-HELPERS FROM PERIPHERAL BLOOD
title_full	CHEMOKINE RECEPTORS AT DISTINCT DIFFERENTIATION STAGES OF T-HELPERS FROM PERIPHERAL BLOOD
title_fullStr	CHEMOKINE RECEPTORS AT DISTINCT DIFFERENTIATION STAGES OF T-HELPERS FROM PERIPHERAL BLOOD
title_full_unstemmed	CHEMOKINE RECEPTORS AT DISTINCT DIFFERENTIATION STAGES OF T-HELPERS FROM PERIPHERAL BLOOD
title_sort	chemokine receptors at distinct differentiation stages of t-helpers from peripheral blood
publisher	SPb RAACI
publishDate	2016
url	https://doaj.org/article/26981136c2054b55858782ab3e0eb3b7
work_keys_str_mv	AT ivkudryavtsev chemokinereceptorsatdistinctdifferentiationstagesofthelpersfromperipheralblood AT agborisov chemokinereceptorsatdistinctdifferentiationstagesofthelpersfromperipheralblood AT iikrobinets chemokinereceptorsatdistinctdifferentiationstagesofthelpersfromperipheralblood AT aasavchenko chemokinereceptorsatdistinctdifferentiationstagesofthelpersfromperipheralblood AT mkserebriakova chemokinereceptorsatdistinctdifferentiationstagesofthelpersfromperipheralblood AT aregatotolian chemokinereceptorsatdistinctdifferentiationstagesofthelpersfromperipheralblood
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CHEMOKINE RECEPTORS AT DISTINCT DIFFERENTIATION STAGES OF T-HELPERS FROM PERIPHERAL BLOOD

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