The level of Ets-1 protein is regulated by poly(ADP-ribose) polymerase-1 (PARP-1) in cancer cells to prevent DNA damage.

Ets-1 is a transcription factor that regulates many genes involved in cancer progression and in tumour invasion. It is a poor prognostic marker for breast, lung, colorectal and ovary carcinomas. Here, we identified poly(ADP-ribose) polymerase-1 (PARP-1) as a novel interaction partner of Ets-1. We sh...

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Autores principales:	Arnaud J Legrand, Souhaila Choul-Li, Corentin Spriet, Thierry Idziorek, Dorothée Vicogne, Hervé Drobecq, Françoise Dantzer, Vincent Villeret, Marc Aumercier
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/269959ef26424e268abc49222b335294
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spelling	oai:doaj.org-article:269959ef26424e268abc49222b3352942021-11-18T07:58:29ZThe level of Ets-1 protein is regulated by poly(ADP-ribose) polymerase-1 (PARP-1) in cancer cells to prevent DNA damage.1932-620310.1371/journal.pone.0055883https://doaj.org/article/269959ef26424e268abc49222b3352942013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23405229/?tool=EBIhttps://doaj.org/toc/1932-6203Ets-1 is a transcription factor that regulates many genes involved in cancer progression and in tumour invasion. It is a poor prognostic marker for breast, lung, colorectal and ovary carcinomas. Here, we identified poly(ADP-ribose) polymerase-1 (PARP-1) as a novel interaction partner of Ets-1. We show that Ets-1 activates, by direct interaction, the catalytic activity of PARP-1 and is then poly(ADP-ribosyl)ated in a DNA-independent manner. The catalytic inhibition of PARP-1 enhanced Ets-1 transcriptional activity and caused its massive accumulation in cell nuclei. Ets-1 expression was correlated with an increase in DNA damage when PARP-1 was inhibited, leading to cancer cell death. Moreover, PARP-1 inhibitors caused only Ets-1-expressing cells to accumulate DNA damage. These results provide new insight into Ets-1 regulation in cancer cells and its link with DNA repair proteins. Furthermore, our findings suggest that PARP-1 inhibitors would be useful in a new therapeutic strategy that specifically targets Ets-1-expressing tumours.Arnaud J LegrandSouhaila Choul-LiCorentin SprietThierry IdziorekDorothée VicogneHervé DrobecqFrançoise DantzerVincent VilleretMarc AumercierPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e55883 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Arnaud J Legrand Souhaila Choul-Li Corentin Spriet Thierry Idziorek Dorothée Vicogne Hervé Drobecq Françoise Dantzer Vincent Villeret Marc Aumercier The level of Ets-1 protein is regulated by poly(ADP-ribose) polymerase-1 (PARP-1) in cancer cells to prevent DNA damage.
description	Ets-1 is a transcription factor that regulates many genes involved in cancer progression and in tumour invasion. It is a poor prognostic marker for breast, lung, colorectal and ovary carcinomas. Here, we identified poly(ADP-ribose) polymerase-1 (PARP-1) as a novel interaction partner of Ets-1. We show that Ets-1 activates, by direct interaction, the catalytic activity of PARP-1 and is then poly(ADP-ribosyl)ated in a DNA-independent manner. The catalytic inhibition of PARP-1 enhanced Ets-1 transcriptional activity and caused its massive accumulation in cell nuclei. Ets-1 expression was correlated with an increase in DNA damage when PARP-1 was inhibited, leading to cancer cell death. Moreover, PARP-1 inhibitors caused only Ets-1-expressing cells to accumulate DNA damage. These results provide new insight into Ets-1 regulation in cancer cells and its link with DNA repair proteins. Furthermore, our findings suggest that PARP-1 inhibitors would be useful in a new therapeutic strategy that specifically targets Ets-1-expressing tumours.
format	article
author	Arnaud J Legrand Souhaila Choul-Li Corentin Spriet Thierry Idziorek Dorothée Vicogne Hervé Drobecq Françoise Dantzer Vincent Villeret Marc Aumercier
author_facet	Arnaud J Legrand Souhaila Choul-Li Corentin Spriet Thierry Idziorek Dorothée Vicogne Hervé Drobecq Françoise Dantzer Vincent Villeret Marc Aumercier
author_sort	Arnaud J Legrand
title	The level of Ets-1 protein is regulated by poly(ADP-ribose) polymerase-1 (PARP-1) in cancer cells to prevent DNA damage.
title_short	The level of Ets-1 protein is regulated by poly(ADP-ribose) polymerase-1 (PARP-1) in cancer cells to prevent DNA damage.
title_full	The level of Ets-1 protein is regulated by poly(ADP-ribose) polymerase-1 (PARP-1) in cancer cells to prevent DNA damage.
title_fullStr	The level of Ets-1 protein is regulated by poly(ADP-ribose) polymerase-1 (PARP-1) in cancer cells to prevent DNA damage.
title_full_unstemmed	The level of Ets-1 protein is regulated by poly(ADP-ribose) polymerase-1 (PARP-1) in cancer cells to prevent DNA damage.
title_sort	level of ets-1 protein is regulated by poly(adp-ribose) polymerase-1 (parp-1) in cancer cells to prevent dna damage.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/269959ef26424e268abc49222b335294
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The level of Ets-1 protein is regulated by poly(ADP-ribose) polymerase-1 (PARP-1) in cancer cells to prevent DNA damage.

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