Surface modifications of silica nanoparticles are crucial for their inert versus proinflammatory and immunomodulatory properties
Viviana Marzaioli,1 Juan Antonio Aguilar-Pimente,1,2 Ingrid Weichenmeier,1 Georg Luxenhofer,3 Martin Wiemann,4 Robert Landsiedel,5 Wendel Wohlleben,5 Stefanie Eiden,6 Martin Mempel,7 Heidrun Behrendt,1 Carsten Schmidt-Weber,1 Jan Gutermuth,1,8 Francesca Alessandrini1 1Center of Allergy and Environm...
Guardado en:
| Autores principales: | , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Dove Medical Press
2014
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/2699b1086bdc4611af97e022449118a1 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:2699b1086bdc4611af97e022449118a1 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:2699b1086bdc4611af97e022449118a12021-12-02T01:04:02ZSurface modifications of silica nanoparticles are crucial for their inert versus proinflammatory and immunomodulatory properties1178-2013https://doaj.org/article/2699b1086bdc4611af97e022449118a12014-06-01T00:00:00Zhttp://www.dovepress.com/surface-modifications-of-silica-nanoparticles-are-crucial-for-their-in-a17112https://doaj.org/toc/1178-2013 Viviana Marzaioli,1 Juan Antonio Aguilar-Pimente,1,2 Ingrid Weichenmeier,1 Georg Luxenhofer,3 Martin Wiemann,4 Robert Landsiedel,5 Wendel Wohlleben,5 Stefanie Eiden,6 Martin Mempel,7 Heidrun Behrendt,1 Carsten Schmidt-Weber,1 Jan Gutermuth,1,8 Francesca Alessandrini1 1Center of Allergy and Environment (ZAUM), Technische Universität and Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Munich, Germany; 2Department of Dermatology and Allergy Biederstein, Technische Universität München (TUM) and German Mouse Clinic, Helmholtz Zentrum München, German Research Center for Environmental Health, Munich-Neuherberg, Germany, 3Institute of Developmental Genetics, Helmholtz Zentrum München, Neuherberg, Germany; 4IBE R&D gGmbH, Münster, Germany; 5BASF, Ludwigshafen, Germany; 6Bayer Technology Services, Leverkusen, Germany; 7Department of Dermatology, Venereology and Allergology, Universitätsmedizin Göttingen (UMG), Göttingen, Germany; 8Department of Dermatology, Vrije Universiteit Brussel, Brussels, Belgium Background: Silica (SiO2) nanoparticles (NPs) are widely used in diverse industrial and biomedical applications. Their applicability depends on surface modifications, which can limit potential health problems. Objective: To assess the potential impact of SiO2 NP exposure and NPs chemical modifications in allergic airway inflammation. Methods: Mice were sensitized by five repetitive intraperitoneal injections of ovalbumin/aluminum hydroxide (1 µg) over 42 days, then intratracheally instilled with plain or modified SiO2 NPs (50 µg/mouse), and subsequently aerosol challenged for 20 minutes with ovalbumin. One or 5 days later, allergic inflammation was evaluated by cell differentiation of bronchoalveolar lavage fluid, lung function and gene expression and histopathology, as well as electron and confocal microscopy of pulmonary tissue. Results: Plain SiO2 NPs induced proinflammatory and immunomodulatory effects in vivo, highlighted by enhanced infiltration of inflammatory cells in the bronchoalveolar lavage fluid, induction of a pulmonary T helper type 2 (Th2) cytokine pattern, differentiation of type 2 macrophages, and by morphological changes in the lung of sensitized mice. These effects were dramatically attenuated using surface-functionalized NPs with amino and phosphate groups, but not with polyethylene glycol. The role of macrophages in taking up SiO2 NPs was confirmed by flow cytometry, confocal microscopy, and gene expression analysis. Conclusion: Our data suggest that amino and phosphate surface modifications, but not polyethylene glycol (PEG), mitigate the proinflammatory and immunomodulatory effect of SiO2 NPs in allergic airway inflammation, paving the way for new strategies in the production of nanomaterials with lower health impact for humans. Keywords: immunomodulation, allergic inflammation, health impactMarzaioli VAguilar-Pimentel JAWeichenmeier ILuxenhofer GWiemann MLandsiedel RWohlleben WEiden SMempel MBehrendt HSchmidt-Weber CGutermuth JAlessandrini FDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 2815-2832 (2014) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine (General) R5-920 |
| spellingShingle |
Medicine (General) R5-920 Marzaioli V Aguilar-Pimentel JA Weichenmeier I Luxenhofer G Wiemann M Landsiedel R Wohlleben W Eiden S Mempel M Behrendt H Schmidt-Weber C Gutermuth J Alessandrini F Surface modifications of silica nanoparticles are crucial for their inert versus proinflammatory and immunomodulatory properties |
| description |
Viviana Marzaioli,1 Juan Antonio Aguilar-Pimente,1,2 Ingrid Weichenmeier,1 Georg Luxenhofer,3 Martin Wiemann,4 Robert Landsiedel,5 Wendel Wohlleben,5 Stefanie Eiden,6 Martin Mempel,7 Heidrun Behrendt,1 Carsten Schmidt-Weber,1 Jan Gutermuth,1,8 Francesca Alessandrini1 1Center of Allergy and Environment (ZAUM), Technische Universität and Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Munich, Germany; 2Department of Dermatology and Allergy Biederstein, Technische Universität München (TUM) and German Mouse Clinic, Helmholtz Zentrum München, German Research Center for Environmental Health, Munich-Neuherberg, Germany, 3Institute of Developmental Genetics, Helmholtz Zentrum München, Neuherberg, Germany; 4IBE R&D gGmbH, Münster, Germany; 5BASF, Ludwigshafen, Germany; 6Bayer Technology Services, Leverkusen, Germany; 7Department of Dermatology, Venereology and Allergology, Universitätsmedizin Göttingen (UMG), Göttingen, Germany; 8Department of Dermatology, Vrije Universiteit Brussel, Brussels, Belgium Background: Silica (SiO2) nanoparticles (NPs) are widely used in diverse industrial and biomedical applications. Their applicability depends on surface modifications, which can limit potential health problems. Objective: To assess the potential impact of SiO2 NP exposure and NPs chemical modifications in allergic airway inflammation. Methods: Mice were sensitized by five repetitive intraperitoneal injections of ovalbumin/aluminum hydroxide (1 µg) over 42 days, then intratracheally instilled with plain or modified SiO2 NPs (50 µg/mouse), and subsequently aerosol challenged for 20 minutes with ovalbumin. One or 5 days later, allergic inflammation was evaluated by cell differentiation of bronchoalveolar lavage fluid, lung function and gene expression and histopathology, as well as electron and confocal microscopy of pulmonary tissue. Results: Plain SiO2 NPs induced proinflammatory and immunomodulatory effects in vivo, highlighted by enhanced infiltration of inflammatory cells in the bronchoalveolar lavage fluid, induction of a pulmonary T helper type 2 (Th2) cytokine pattern, differentiation of type 2 macrophages, and by morphological changes in the lung of sensitized mice. These effects were dramatically attenuated using surface-functionalized NPs with amino and phosphate groups, but not with polyethylene glycol. The role of macrophages in taking up SiO2 NPs was confirmed by flow cytometry, confocal microscopy, and gene expression analysis. Conclusion: Our data suggest that amino and phosphate surface modifications, but not polyethylene glycol (PEG), mitigate the proinflammatory and immunomodulatory effect of SiO2 NPs in allergic airway inflammation, paving the way for new strategies in the production of nanomaterials with lower health impact for humans. Keywords: immunomodulation, allergic inflammation, health impact |
| format |
article |
| author |
Marzaioli V Aguilar-Pimentel JA Weichenmeier I Luxenhofer G Wiemann M Landsiedel R Wohlleben W Eiden S Mempel M Behrendt H Schmidt-Weber C Gutermuth J Alessandrini F |
| author_facet |
Marzaioli V Aguilar-Pimentel JA Weichenmeier I Luxenhofer G Wiemann M Landsiedel R Wohlleben W Eiden S Mempel M Behrendt H Schmidt-Weber C Gutermuth J Alessandrini F |
| author_sort |
Marzaioli V |
| title |
Surface modifications of silica nanoparticles are crucial for their inert versus proinflammatory and immunomodulatory properties |
| title_short |
Surface modifications of silica nanoparticles are crucial for their inert versus proinflammatory and immunomodulatory properties |
| title_full |
Surface modifications of silica nanoparticles are crucial for their inert versus proinflammatory and immunomodulatory properties |
| title_fullStr |
Surface modifications of silica nanoparticles are crucial for their inert versus proinflammatory and immunomodulatory properties |
| title_full_unstemmed |
Surface modifications of silica nanoparticles are crucial for their inert versus proinflammatory and immunomodulatory properties |
| title_sort |
surface modifications of silica nanoparticles are crucial for their inert versus proinflammatory and immunomodulatory properties |
| publisher |
Dove Medical Press |
| publishDate |
2014 |
| url |
https://doaj.org/article/2699b1086bdc4611af97e022449118a1 |
| work_keys_str_mv |
AT marzaioliv surfacemodificationsofsilicananoparticlesarecrucialfortheirinertversusproinflammatoryandimmunomodulatoryproperties AT aguilarpimentelja surfacemodificationsofsilicananoparticlesarecrucialfortheirinertversusproinflammatoryandimmunomodulatoryproperties AT weichenmeieri surfacemodificationsofsilicananoparticlesarecrucialfortheirinertversusproinflammatoryandimmunomodulatoryproperties AT luxenhoferg surfacemodificationsofsilicananoparticlesarecrucialfortheirinertversusproinflammatoryandimmunomodulatoryproperties AT wiemannm surfacemodificationsofsilicananoparticlesarecrucialfortheirinertversusproinflammatoryandimmunomodulatoryproperties AT landsiedelr surfacemodificationsofsilicananoparticlesarecrucialfortheirinertversusproinflammatoryandimmunomodulatoryproperties AT wohllebenw surfacemodificationsofsilicananoparticlesarecrucialfortheirinertversusproinflammatoryandimmunomodulatoryproperties AT eidens surfacemodificationsofsilicananoparticlesarecrucialfortheirinertversusproinflammatoryandimmunomodulatoryproperties AT mempelm surfacemodificationsofsilicananoparticlesarecrucialfortheirinertversusproinflammatoryandimmunomodulatoryproperties AT behrendth surfacemodificationsofsilicananoparticlesarecrucialfortheirinertversusproinflammatoryandimmunomodulatoryproperties AT schmidtweberc surfacemodificationsofsilicananoparticlesarecrucialfortheirinertversusproinflammatoryandimmunomodulatoryproperties AT gutermuthj surfacemodificationsofsilicananoparticlesarecrucialfortheirinertversusproinflammatoryandimmunomodulatoryproperties AT alessandrinif surfacemodificationsofsilicananoparticlesarecrucialfortheirinertversusproinflammatoryandimmunomodulatoryproperties |
| _version_ |
1718403305180233728 |