A potential diagnostic serum immunological marker panel to differentiate between primary and secondary knee osteoarthritis.

Inflammation contributes to knee osteoarthritis (KOA) where many immunological mediators participate in its initiation and progression. Most clinicians manage primary (pKOA) and secondary osteoarthritis (sKOA) alike. Whether immunological profiles of pKOA and sKOA differ remains obscure. Hence, we a...

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Autores principales:	Sakuni Rankothgedera, Inoshi Atukorala, Chandrika Fernando, Duminda Munidasa, Lalith Wijayaratne, Preethi Udagama
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/26a73833bdde4e1a9cfaa4ac292b05af
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spelling	oai:doaj.org-article:26a73833bdde4e1a9cfaa4ac292b05af2021-12-02T20:14:27ZA potential diagnostic serum immunological marker panel to differentiate between primary and secondary knee osteoarthritis.1932-620310.1371/journal.pone.0257507https://doaj.org/article/26a73833bdde4e1a9cfaa4ac292b05af2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0257507https://doaj.org/toc/1932-6203Inflammation contributes to knee osteoarthritis (KOA) where many immunological mediators participate in its initiation and progression. Most clinicians manage primary (pKOA) and secondary osteoarthritis (sKOA) alike. Whether immunological profiles of pKOA and sKOA differ remains obscure. Hence, we aimed to differentially identify potential serum immunologic diagnostic markers of pKOA and of sKOA. This case control study used 46 KOA patients (pKOA, n = 30; sKOA, n = 16), and 60 age, gender matched controls (normal healthy, n = 30; systemic lupus erythematosus [SLE] disease controls, n = 30) where serum was assayed for cytokines (TNF-α, IL-1β, IL-6, IL-10) and nitric oxide derivatives (NOx). Sandwich ELISA assessed cytokine levels, while the 'Griess assay' quantified NOx levels. The diagnostic accuracy of optimal marker combinations was evaluated by the CombiROC web tool. Compared with pKOA, sKOA serum displayed significantly elevated levels of pro inflammatory cytokines (TNF-α, IL-1β, IL-6) with a concurrent decrease in the anti-inflammatory cytokine, IL-10 (P<0.05). This was reiterated by significantly higher Th1:Th2 (TNF-α: IL-10) serum cytokine ratio observed in sKOA compared to that of pKOA. The CombiROC curves identified TNF-α, IL-1β, IL-6 and NOx as the best performing panel of potential diagnostic markers to discriminate pKOA from control groups (~97% accuracy, 90% Sensitivity [SE] and 98% specificity [SP]), while TNF-α, IL-1β and IL-6 discriminated sKOA from control groups (~100% accuracy, 100% SE, and 98% SP). The study identified discrete serum immune biomarker panels to differentiate between pKOA (TNF-α, IL-1β, IL-6 and NOx) and sKOA (TNF-α, IL-1β and IL-6). These findings may assist in developing distinct therapeutic agents for the two types of KOA.Sakuni RankothgederaInoshi AtukoralaChandrika FernandoDuminda MunidasaLalith WijayaratnePreethi UdagamaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0257507 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sakuni Rankothgedera Inoshi Atukorala Chandrika Fernando Duminda Munidasa Lalith Wijayaratne Preethi Udagama A potential diagnostic serum immunological marker panel to differentiate between primary and secondary knee osteoarthritis.
description	Inflammation contributes to knee osteoarthritis (KOA) where many immunological mediators participate in its initiation and progression. Most clinicians manage primary (pKOA) and secondary osteoarthritis (sKOA) alike. Whether immunological profiles of pKOA and sKOA differ remains obscure. Hence, we aimed to differentially identify potential serum immunologic diagnostic markers of pKOA and of sKOA. This case control study used 46 KOA patients (pKOA, n = 30; sKOA, n = 16), and 60 age, gender matched controls (normal healthy, n = 30; systemic lupus erythematosus [SLE] disease controls, n = 30) where serum was assayed for cytokines (TNF-α, IL-1β, IL-6, IL-10) and nitric oxide derivatives (NOx). Sandwich ELISA assessed cytokine levels, while the 'Griess assay' quantified NOx levels. The diagnostic accuracy of optimal marker combinations was evaluated by the CombiROC web tool. Compared with pKOA, sKOA serum displayed significantly elevated levels of pro inflammatory cytokines (TNF-α, IL-1β, IL-6) with a concurrent decrease in the anti-inflammatory cytokine, IL-10 (P<0.05). This was reiterated by significantly higher Th1:Th2 (TNF-α: IL-10) serum cytokine ratio observed in sKOA compared to that of pKOA. The CombiROC curves identified TNF-α, IL-1β, IL-6 and NOx as the best performing panel of potential diagnostic markers to discriminate pKOA from control groups (~97% accuracy, 90% Sensitivity [SE] and 98% specificity [SP]), while TNF-α, IL-1β and IL-6 discriminated sKOA from control groups (~100% accuracy, 100% SE, and 98% SP). The study identified discrete serum immune biomarker panels to differentiate between pKOA (TNF-α, IL-1β, IL-6 and NOx) and sKOA (TNF-α, IL-1β and IL-6). These findings may assist in developing distinct therapeutic agents for the two types of KOA.
format	article
author	Sakuni Rankothgedera Inoshi Atukorala Chandrika Fernando Duminda Munidasa Lalith Wijayaratne Preethi Udagama
author_facet	Sakuni Rankothgedera Inoshi Atukorala Chandrika Fernando Duminda Munidasa Lalith Wijayaratne Preethi Udagama
author_sort	Sakuni Rankothgedera
title	A potential diagnostic serum immunological marker panel to differentiate between primary and secondary knee osteoarthritis.
title_short	A potential diagnostic serum immunological marker panel to differentiate between primary and secondary knee osteoarthritis.
title_full	A potential diagnostic serum immunological marker panel to differentiate between primary and secondary knee osteoarthritis.
title_fullStr	A potential diagnostic serum immunological marker panel to differentiate between primary and secondary knee osteoarthritis.
title_full_unstemmed	A potential diagnostic serum immunological marker panel to differentiate between primary and secondary knee osteoarthritis.
title_sort	potential diagnostic serum immunological marker panel to differentiate between primary and secondary knee osteoarthritis.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/26a73833bdde4e1a9cfaa4ac292b05af
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A potential diagnostic serum immunological marker panel to differentiate between primary and secondary knee osteoarthritis.

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