Effect of Pasteurella multocida Soluble Antigen Stimulation on the In Vitro Response of Peripheral Blood Mononuclear Cells of Holstein Calves

The expressions of cytokines mRNA, including interleukin-4 (IL-4), interleukin- 17A (IL-17A) and interferon-gamma (IFN-γ), their master regulatory transcription factors, and signal transducers and activator of transcription (STAT) stimulated in vitro with Pasteurella (P.) multocida soluble antigen w...

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Autores principales:	Ohtsuka Hiromichi, Inoue Maki, Maeda Yosuke, Tanabe Taishi, Tajima Motoshi
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2018
Materias:	calves cytokine immune function pasteurella multocida Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/26a75da17e0a468bb1d33e2bcb9f23ed
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spelling	oai:doaj.org-article:26a75da17e0a468bb1d33e2bcb9f23ed2021-11-17T21:27:52ZEffect of Pasteurella multocida Soluble Antigen Stimulation on the In Vitro Response of Peripheral Blood Mononuclear Cells of Holstein Calves1820-744810.2478/acve-2018-0014https://doaj.org/article/26a75da17e0a468bb1d33e2bcb9f23ed2018-06-01T00:00:00Zhttps://doi.org/10.2478/acve-2018-0014https://doaj.org/toc/1820-7448The expressions of cytokines mRNA, including interleukin-4 (IL-4), interleukin- 17A (IL-17A) and interferon-gamma (IFN-γ), their master regulatory transcription factors, and signal transducers and activator of transcription (STAT) stimulated in vitro with Pasteurella (P.) multocida soluble antigen were examined in peripheral blood mononuclear cells (PBMC) from Holstein calves. The healthy Holstein calves were divided into three groups; 2 weeks old (2W Group, N=8), 6 weeks old (6W Group, N=8), and 10 weeks old (10W Group, N=8). PBMC were stimulated in vitro by soluble antigen of P. multocida. There were significantly lower expressions of IFN-γ, IL-4, and STAT-6 mRNA of PBMC stimulated with P. multocida soluble antigen in the 2W Group compared to that in the 10W Group. Expression of IL-17A and IFN-γ in PBMC stimulated with P. multocida soluble antigen were significantly higher compared with the PBMC without stimulation in the 6W groups. The results of the present study demonstrated that 2W old calves had decreased cytokine expression of PBMC when in vitro stimulated with P. multocida soluble antigen in vitro.Ohtsuka HiromichiInoue MakiMaeda YosukeTanabe TaishiTajima MotoshiSciendoarticlecalvescytokineimmune functionpasteurella multocidaVeterinary medicineSF600-1100ENActa Veterinaria, Vol 68, Iss 2, Pp 201-210 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	calves cytokine immune function pasteurella multocida Veterinary medicine SF600-1100
spellingShingle	calves cytokine immune function pasteurella multocida Veterinary medicine SF600-1100 Ohtsuka Hiromichi Inoue Maki Maeda Yosuke Tanabe Taishi Tajima Motoshi Effect of Pasteurella multocida Soluble Antigen Stimulation on the In Vitro Response of Peripheral Blood Mononuclear Cells of Holstein Calves
description	The expressions of cytokines mRNA, including interleukin-4 (IL-4), interleukin- 17A (IL-17A) and interferon-gamma (IFN-γ), their master regulatory transcription factors, and signal transducers and activator of transcription (STAT) stimulated in vitro with Pasteurella (P.) multocida soluble antigen were examined in peripheral blood mononuclear cells (PBMC) from Holstein calves. The healthy Holstein calves were divided into three groups; 2 weeks old (2W Group, N=8), 6 weeks old (6W Group, N=8), and 10 weeks old (10W Group, N=8). PBMC were stimulated in vitro by soluble antigen of P. multocida. There were significantly lower expressions of IFN-γ, IL-4, and STAT-6 mRNA of PBMC stimulated with P. multocida soluble antigen in the 2W Group compared to that in the 10W Group. Expression of IL-17A and IFN-γ in PBMC stimulated with P. multocida soluble antigen were significantly higher compared with the PBMC without stimulation in the 6W groups. The results of the present study demonstrated that 2W old calves had decreased cytokine expression of PBMC when in vitro stimulated with P. multocida soluble antigen in vitro.
format	article
author	Ohtsuka Hiromichi Inoue Maki Maeda Yosuke Tanabe Taishi Tajima Motoshi
author_facet	Ohtsuka Hiromichi Inoue Maki Maeda Yosuke Tanabe Taishi Tajima Motoshi
author_sort	Ohtsuka Hiromichi
title	Effect of Pasteurella multocida Soluble Antigen Stimulation on the In Vitro Response of Peripheral Blood Mononuclear Cells of Holstein Calves
title_short	Effect of Pasteurella multocida Soluble Antigen Stimulation on the In Vitro Response of Peripheral Blood Mononuclear Cells of Holstein Calves
title_full	Effect of Pasteurella multocida Soluble Antigen Stimulation on the In Vitro Response of Peripheral Blood Mononuclear Cells of Holstein Calves
title_fullStr	Effect of Pasteurella multocida Soluble Antigen Stimulation on the In Vitro Response of Peripheral Blood Mononuclear Cells of Holstein Calves
title_full_unstemmed	Effect of Pasteurella multocida Soluble Antigen Stimulation on the In Vitro Response of Peripheral Blood Mononuclear Cells of Holstein Calves
title_sort	effect of pasteurella multocida soluble antigen stimulation on the in vitro response of peripheral blood mononuclear cells of holstein calves
publisher	Sciendo
publishDate	2018
url	https://doaj.org/article/26a75da17e0a468bb1d33e2bcb9f23ed
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Effect of Pasteurella multocida Soluble Antigen Stimulation on the In Vitro Response of Peripheral Blood Mononuclear Cells of Holstein Calves

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