STING mediates immune responses in the closest living relatives of animals

Animals have evolved unique repertoires of innate immune genes and pathways that provide their first line of defense against pathogens. To reconstruct the ancestry of animal innate immunity, we have developed the choanoflagellate Monosiga brevicollis, one of the closest living relatives of animals,...

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Autores principales:	Arielle Woznica, Ashwani Kumar, Carolyn R Sturge, Chao Xing, Nicole King, Julie K Pfeiffer
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	choanoflagellates Monosiga brevicollis Pseudomonas aeruginosa STING Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/26a7afda179541cc8e62ad717b28db18
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id	oai:doaj.org-article:26a7afda179541cc8e62ad717b28db18
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spelling	oai:doaj.org-article:26a7afda179541cc8e62ad717b28db182021-11-15T16:40:20ZSTING mediates immune responses in the closest living relatives of animals10.7554/eLife.704362050-084Xe70436https://doaj.org/article/26a7afda179541cc8e62ad717b28db182021-11-01T00:00:00Zhttps://elifesciences.org/articles/70436https://doaj.org/toc/2050-084XAnimals have evolved unique repertoires of innate immune genes and pathways that provide their first line of defense against pathogens. To reconstruct the ancestry of animal innate immunity, we have developed the choanoflagellate Monosiga brevicollis, one of the closest living relatives of animals, as a model for studying mechanisms underlying pathogen recognition and immune response. We found that M. brevicollis is killed by exposure to Pseudomonas aeruginosa bacteria. Moreover, M. brevicollis expresses STING, which, in animals, activates innate immune pathways in response to cyclic dinucleotides during pathogen sensing. M. brevicollis STING increases the susceptibility of M. brevicollis to P. aeruginosa-induced cell death and is required for responding to the cyclic dinucleotide 2’3’ cGAMP. Furthermore, similar to animals, autophagic signaling in M. brevicollis is induced by 2’3’ cGAMP in a STING-dependent manner. This study provides evidence for a pre-animal role for STING in antibacterial immunity and establishes M. brevicollis as a model system for the study of immune responses.Arielle WoznicaAshwani KumarCarolyn R SturgeChao XingNicole KingJulie K PfeiffereLife Sciences Publications LtdarticlechoanoflagellatesMonosiga brevicollisPseudomonas aeruginosaSTINGMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	choanoflagellates Monosiga brevicollis Pseudomonas aeruginosa STING Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	choanoflagellates Monosiga brevicollis Pseudomonas aeruginosa STING Medicine R Science Q Biology (General) QH301-705.5 Arielle Woznica Ashwani Kumar Carolyn R Sturge Chao Xing Nicole King Julie K Pfeiffer STING mediates immune responses in the closest living relatives of animals
description	Animals have evolved unique repertoires of innate immune genes and pathways that provide their first line of defense against pathogens. To reconstruct the ancestry of animal innate immunity, we have developed the choanoflagellate Monosiga brevicollis, one of the closest living relatives of animals, as a model for studying mechanisms underlying pathogen recognition and immune response. We found that M. brevicollis is killed by exposure to Pseudomonas aeruginosa bacteria. Moreover, M. brevicollis expresses STING, which, in animals, activates innate immune pathways in response to cyclic dinucleotides during pathogen sensing. M. brevicollis STING increases the susceptibility of M. brevicollis to P. aeruginosa-induced cell death and is required for responding to the cyclic dinucleotide 2’3’ cGAMP. Furthermore, similar to animals, autophagic signaling in M. brevicollis is induced by 2’3’ cGAMP in a STING-dependent manner. This study provides evidence for a pre-animal role for STING in antibacterial immunity and establishes M. brevicollis as a model system for the study of immune responses.
format	article
author	Arielle Woznica Ashwani Kumar Carolyn R Sturge Chao Xing Nicole King Julie K Pfeiffer
author_facet	Arielle Woznica Ashwani Kumar Carolyn R Sturge Chao Xing Nicole King Julie K Pfeiffer
author_sort	Arielle Woznica
title	STING mediates immune responses in the closest living relatives of animals
title_short	STING mediates immune responses in the closest living relatives of animals
title_full	STING mediates immune responses in the closest living relatives of animals
title_fullStr	STING mediates immune responses in the closest living relatives of animals
title_full_unstemmed	STING mediates immune responses in the closest living relatives of animals
title_sort	sting mediates immune responses in the closest living relatives of animals
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/26a7afda179541cc8e62ad717b28db18
work_keys_str_mv	AT ariellewoznica stingmediatesimmuneresponsesintheclosestlivingrelativesofanimals AT ashwanikumar stingmediatesimmuneresponsesintheclosestlivingrelativesofanimals AT carolynrsturge stingmediatesimmuneresponsesintheclosestlivingrelativesofanimals AT chaoxing stingmediatesimmuneresponsesintheclosestlivingrelativesofanimals AT nicoleking stingmediatesimmuneresponsesintheclosestlivingrelativesofanimals AT juliekpfeiffer stingmediatesimmuneresponsesintheclosestlivingrelativesofanimals
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STING mediates immune responses in the closest living relatives of animals

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