Agar with embedded channels to study root growth

Abstract Agar have long been used as a growth media for plants. Here, we made agar media with embedded fluidic channels to study the effect of exposure to nutrient solution on root growth and pull-out force. Black Eye bean (Vigna Unguiculata) and Mung bean (Vigna Radiata) were used in this study due...

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Autores principales:	Azlan Abdul Aziz, Kai Boon Lim, Ena Kartina Abdul Rahman, Muhammad Hanafiah Nurmawati, Abu Samah Zuruzi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/26b4337acd314bba855548f8aeb75eb7
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spelling	oai:doaj.org-article:26b4337acd314bba855548f8aeb75eb72021-12-02T19:02:32ZAgar with embedded channels to study root growth10.1038/s41598-020-71076-w2045-2322https://doaj.org/article/26b4337acd314bba855548f8aeb75eb72020-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-71076-whttps://doaj.org/toc/2045-2322Abstract Agar have long been used as a growth media for plants. Here, we made agar media with embedded fluidic channels to study the effect of exposure to nutrient solution on root growth and pull-out force. Black Eye bean (Vigna Unguiculata) and Mung bean (Vigna Radiata) were used in this study due to their rapid root development. Agar media were fabricated using casting process with removable cores to form channels which were subsequently filled with nutrient solution. Upon germination, beans were transplanted onto the agar media and allowed to grow. Pull-out force was determined at 96, 120 and 144 h after germination by applying a force on the hypocotyl above the gel surface. The effect of nutrients was investigated by comparing corresponding data obtained from control plants which have not been exposed to nutrient solution. Pull-out force of Black Eye bean plantlets grown in agar with nutrient solution in channels was greater than those grown in gel without nutrients and was 110% greater after 144 h of germination. Pull-out force of Mung bean plantlets grown in agar with and without nutrient solution was similar. Tap root lengths of Black Eye bean and Mung Bean plantlets grown in agar with nutrient solution are shorter than those grown without nutrient. Azlan Abdul AzizKai Boon LimEna Kartina Abdul RahmanMuhammad Hanafiah NurmawatiAbu Samah ZuruziNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Azlan Abdul Aziz Kai Boon Lim Ena Kartina Abdul Rahman Muhammad Hanafiah Nurmawati Abu Samah Zuruzi Agar with embedded channels to study root growth
description	Abstract Agar have long been used as a growth media for plants. Here, we made agar media with embedded fluidic channels to study the effect of exposure to nutrient solution on root growth and pull-out force. Black Eye bean (Vigna Unguiculata) and Mung bean (Vigna Radiata) were used in this study due to their rapid root development. Agar media were fabricated using casting process with removable cores to form channels which were subsequently filled with nutrient solution. Upon germination, beans were transplanted onto the agar media and allowed to grow. Pull-out force was determined at 96, 120 and 144 h after germination by applying a force on the hypocotyl above the gel surface. The effect of nutrients was investigated by comparing corresponding data obtained from control plants which have not been exposed to nutrient solution. Pull-out force of Black Eye bean plantlets grown in agar with nutrient solution in channels was greater than those grown in gel without nutrients and was 110% greater after 144 h of germination. Pull-out force of Mung bean plantlets grown in agar with and without nutrient solution was similar. Tap root lengths of Black Eye bean and Mung Bean plantlets grown in agar with nutrient solution are shorter than those grown without nutrient.
format	article
author	Azlan Abdul Aziz Kai Boon Lim Ena Kartina Abdul Rahman Muhammad Hanafiah Nurmawati Abu Samah Zuruzi
author_facet	Azlan Abdul Aziz Kai Boon Lim Ena Kartina Abdul Rahman Muhammad Hanafiah Nurmawati Abu Samah Zuruzi
author_sort	Azlan Abdul Aziz
title	Agar with embedded channels to study root growth
title_short	Agar with embedded channels to study root growth
title_full	Agar with embedded channels to study root growth
title_fullStr	Agar with embedded channels to study root growth
title_full_unstemmed	Agar with embedded channels to study root growth
title_sort	agar with embedded channels to study root growth
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/26b4337acd314bba855548f8aeb75eb7
work_keys_str_mv	AT azlanabdulaziz agarwithembeddedchannelstostudyrootgrowth AT kaiboonlim agarwithembeddedchannelstostudyrootgrowth AT enakartinaabdulrahman agarwithembeddedchannelstostudyrootgrowth AT muhammadhanafiahnurmawati agarwithembeddedchannelstostudyrootgrowth AT abusamahzuruzi agarwithembeddedchannelstostudyrootgrowth
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Agar with embedded channels to study root growth

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