Detection of a bocavirus circular genome in fecal specimens from children with acute diarrhea in Beijing, China.

To determine if human bocavirus 2 (HBoV2) has a circular genome similar to the head-to-tail sequence of HBoV1 and the episomal form of HBoV3, 15 HBoV2 positive samples identified from 553 stool specimens from children with acute diarrhea were tested for a head-to-tail sequence using TaqMan-based rea...

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Autores principales:	Hui Zhao, Linqing Zhao, Yu Sun, Yuan Qian, Liying Liu, Liping Jia, You Zhang, Huijin Dong
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/26b946449bef44fdb4688021a1fae983
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id	oai:doaj.org-article:26b946449bef44fdb4688021a1fae983
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spelling	oai:doaj.org-article:26b946449bef44fdb4688021a1fae9832021-11-18T08:10:08ZDetection of a bocavirus circular genome in fecal specimens from children with acute diarrhea in Beijing, China.1932-620310.1371/journal.pone.0048980https://doaj.org/article/26b946449bef44fdb4688021a1fae9832012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23133667/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203To determine if human bocavirus 2 (HBoV2) has a circular genome similar to the head-to-tail sequence of HBoV1 and the episomal form of HBoV3, 15 HBoV2 positive samples identified from 553 stool specimens from children with acute diarrhea were tested for a head-to-tail sequence using TaqMan-based real-time PCR. A circular genome with a head-to-tail sequence was identified in one (BJQ435) out of 15 samples tested by nested PCR. The complete circular genome of HBoV2-C1 (BJQ435) was 5307 nt in length and was flanked with a 520 nt-long terminal non-coding region (NCR). The secondary structure of HBoV2 -C1 had some differences compared to HBoV3-E1 (JN086998). Our study indicates that the HBoV genome exists in the form of a head-to-tail monomer and provides more information for understanding the HBoV replication mechanism.Hui ZhaoLinqing ZhaoYu SunYuan QianLiying LiuLiping JiaYou ZhangHuijin DongPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e48980 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hui Zhao Linqing Zhao Yu Sun Yuan Qian Liying Liu Liping Jia You Zhang Huijin Dong Detection of a bocavirus circular genome in fecal specimens from children with acute diarrhea in Beijing, China.
description	To determine if human bocavirus 2 (HBoV2) has a circular genome similar to the head-to-tail sequence of HBoV1 and the episomal form of HBoV3, 15 HBoV2 positive samples identified from 553 stool specimens from children with acute diarrhea were tested for a head-to-tail sequence using TaqMan-based real-time PCR. A circular genome with a head-to-tail sequence was identified in one (BJQ435) out of 15 samples tested by nested PCR. The complete circular genome of HBoV2-C1 (BJQ435) was 5307 nt in length and was flanked with a 520 nt-long terminal non-coding region (NCR). The secondary structure of HBoV2 -C1 had some differences compared to HBoV3-E1 (JN086998). Our study indicates that the HBoV genome exists in the form of a head-to-tail monomer and provides more information for understanding the HBoV replication mechanism.
format	article
author	Hui Zhao Linqing Zhao Yu Sun Yuan Qian Liying Liu Liping Jia You Zhang Huijin Dong
author_facet	Hui Zhao Linqing Zhao Yu Sun Yuan Qian Liying Liu Liping Jia You Zhang Huijin Dong
author_sort	Hui Zhao
title	Detection of a bocavirus circular genome in fecal specimens from children with acute diarrhea in Beijing, China.
title_short	Detection of a bocavirus circular genome in fecal specimens from children with acute diarrhea in Beijing, China.
title_full	Detection of a bocavirus circular genome in fecal specimens from children with acute diarrhea in Beijing, China.
title_fullStr	Detection of a bocavirus circular genome in fecal specimens from children with acute diarrhea in Beijing, China.
title_full_unstemmed	Detection of a bocavirus circular genome in fecal specimens from children with acute diarrhea in Beijing, China.
title_sort	detection of a bocavirus circular genome in fecal specimens from children with acute diarrhea in beijing, china.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/26b946449bef44fdb4688021a1fae983
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Detection of a bocavirus circular genome in fecal specimens from children with acute diarrhea in Beijing, China.

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