Is thermosensing property of RNA thermometers unique?

A large number of studies have been dedicated to identify the structural and sequence based features of RNA thermometers, mRNAs that regulate their translation initiation rate with temperature. It has been shown that the melting of the ribosome-binding site (RBS) plays a prominent role in this therm...

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Autores principales:	Premal Shah, Michael A Gilchrist
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/26bce4f6e468467c8197bcdfdfacce5c
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spelling	oai:doaj.org-article:26bce4f6e468467c8197bcdfdfacce5c2021-12-02T20:20:20ZIs thermosensing property of RNA thermometers unique?1932-620310.1371/journal.pone.0011308https://doaj.org/article/26bce4f6e468467c8197bcdfdfacce5c2010-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20625392/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203A large number of studies have been dedicated to identify the structural and sequence based features of RNA thermometers, mRNAs that regulate their translation initiation rate with temperature. It has been shown that the melting of the ribosome-binding site (RBS) plays a prominent role in this thermosensing process. However, little is known as to how widespread this melting phenomenon is as earlier studies on the subject have worked with a small sample of known RNA thermometers. We have developed a novel method of studying the melting of RNAs with temperature by computationally sampling the distribution of the RNA structures at various temperatures using the RNA folding software Vienna. In this study, we compared the thermosensing property of 100 randomly selected mRNAs and three well known thermometers--rpoH, ibpA and agsA sequences from E. coli. We also compared the rpoH sequences from 81 mesophilic proteobacteria. Although both rpoH and ibpA show a higher rate of melting at their RBS compared with the mean of non-thermometers, contrary to our expectations these higher rates are not significant. Surprisingly, we also do not find any significant differences between rpoH thermometers from other gamma-proteobacteria and E. coli non-thermometers.Premal ShahMichael A GilchristPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 7, p e11308 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Premal Shah Michael A Gilchrist Is thermosensing property of RNA thermometers unique?
description	A large number of studies have been dedicated to identify the structural and sequence based features of RNA thermometers, mRNAs that regulate their translation initiation rate with temperature. It has been shown that the melting of the ribosome-binding site (RBS) plays a prominent role in this thermosensing process. However, little is known as to how widespread this melting phenomenon is as earlier studies on the subject have worked with a small sample of known RNA thermometers. We have developed a novel method of studying the melting of RNAs with temperature by computationally sampling the distribution of the RNA structures at various temperatures using the RNA folding software Vienna. In this study, we compared the thermosensing property of 100 randomly selected mRNAs and three well known thermometers--rpoH, ibpA and agsA sequences from E. coli. We also compared the rpoH sequences from 81 mesophilic proteobacteria. Although both rpoH and ibpA show a higher rate of melting at their RBS compared with the mean of non-thermometers, contrary to our expectations these higher rates are not significant. Surprisingly, we also do not find any significant differences between rpoH thermometers from other gamma-proteobacteria and E. coli non-thermometers.
format	article
author	Premal Shah Michael A Gilchrist
author_facet	Premal Shah Michael A Gilchrist
author_sort	Premal Shah
title	Is thermosensing property of RNA thermometers unique?
title_short	Is thermosensing property of RNA thermometers unique?
title_full	Is thermosensing property of RNA thermometers unique?
title_fullStr	Is thermosensing property of RNA thermometers unique?
title_full_unstemmed	Is thermosensing property of RNA thermometers unique?
title_sort	is thermosensing property of rna thermometers unique?
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/26bce4f6e468467c8197bcdfdfacce5c
work_keys_str_mv	AT premalshah isthermosensingpropertyofrnathermometersunique AT michaelagilchrist isthermosensingpropertyofrnathermometersunique
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Is thermosensing property of RNA thermometers unique?

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