In Vivo Ribbon Mobility and Turnover of Ribeye at Zebrafish Hair Cell Synapses

Abstract Ribbons are presynaptic structures that mediate synaptic vesicle release in some sensory cells of the auditory and visual systems. Although composed predominately of the protein Ribeye, very little is known about the structural dynamics of ribbons. Here we describe the in vivo mobility and...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Cole W. Graydon, Uri Manor, Katie S. Kindt
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/26bdf68c27654ec8aca226d0987d5c8c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:26bdf68c27654ec8aca226d0987d5c8c
record_format	dspace
spelling	oai:doaj.org-article:26bdf68c27654ec8aca226d0987d5c8c2021-12-02T15:06:05ZIn Vivo Ribbon Mobility and Turnover of Ribeye at Zebrafish Hair Cell Synapses10.1038/s41598-017-07940-z2045-2322https://doaj.org/article/26bdf68c27654ec8aca226d0987d5c8c2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07940-zhttps://doaj.org/toc/2045-2322Abstract Ribbons are presynaptic structures that mediate synaptic vesicle release in some sensory cells of the auditory and visual systems. Although composed predominately of the protein Ribeye, very little is known about the structural dynamics of ribbons. Here we describe the in vivo mobility and turnover of Ribeye at hair cell ribbon synapses by monitoring fluorescence recovery after photobleaching (FRAP) in transgenic zebrafish with GFP-tagged Ribeye. We show that Ribeye can exchange between halves of a ribbon within ~1 minute in a manner that is consistent with a simple diffusion mechanism. In contrast, exchange of Ribeye between other ribbons via the cell’s cytoplasm takes several hours.Cole W. GraydonUri ManorKatie S. KindtNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cole W. Graydon Uri Manor Katie S. Kindt In Vivo Ribbon Mobility and Turnover of Ribeye at Zebrafish Hair Cell Synapses
description	Abstract Ribbons are presynaptic structures that mediate synaptic vesicle release in some sensory cells of the auditory and visual systems. Although composed predominately of the protein Ribeye, very little is known about the structural dynamics of ribbons. Here we describe the in vivo mobility and turnover of Ribeye at hair cell ribbon synapses by monitoring fluorescence recovery after photobleaching (FRAP) in transgenic zebrafish with GFP-tagged Ribeye. We show that Ribeye can exchange between halves of a ribbon within ~1 minute in a manner that is consistent with a simple diffusion mechanism. In contrast, exchange of Ribeye between other ribbons via the cell’s cytoplasm takes several hours.
format	article
author	Cole W. Graydon Uri Manor Katie S. Kindt
author_facet	Cole W. Graydon Uri Manor Katie S. Kindt
author_sort	Cole W. Graydon
title	In Vivo Ribbon Mobility and Turnover of Ribeye at Zebrafish Hair Cell Synapses
title_short	In Vivo Ribbon Mobility and Turnover of Ribeye at Zebrafish Hair Cell Synapses
title_full	In Vivo Ribbon Mobility and Turnover of Ribeye at Zebrafish Hair Cell Synapses
title_fullStr	In Vivo Ribbon Mobility and Turnover of Ribeye at Zebrafish Hair Cell Synapses
title_full_unstemmed	In Vivo Ribbon Mobility and Turnover of Ribeye at Zebrafish Hair Cell Synapses
title_sort	in vivo ribbon mobility and turnover of ribeye at zebrafish hair cell synapses
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/26bdf68c27654ec8aca226d0987d5c8c
work_keys_str_mv	AT colewgraydon invivoribbonmobilityandturnoverofribeyeatzebrafishhaircellsynapses AT urimanor invivoribbonmobilityandturnoverofribeyeatzebrafishhaircellsynapses AT katieskindt invivoribbonmobilityandturnoverofribeyeatzebrafishhaircellsynapses
_version_	1718388566776610816

In Vivo Ribbon Mobility and Turnover of Ribeye at Zebrafish Hair Cell Synapses

Ejemplares similares