The zinc transporter ZIP9 (Slc39a9) regulates zinc dynamics essential to egg activation in zebrafish

Abstract The zinc transporter ZIP9 (SLC39A9) was recently characterized as a membrane androgen receptor in various teleost and mammalian cell models. ZIP9 shows the highest expression in ovaries of teleosts, a tissue in which both androgen signaling and zinc dynamics have significant roles. To exami...

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Autores principales:	Aubrey Converse, Peter Thomas
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/26c9ba675b2d41c7be443925d056b183
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spelling	oai:doaj.org-article:26c9ba675b2d41c7be443925d056b1832021-12-02T18:48:01ZThe zinc transporter ZIP9 (Slc39a9) regulates zinc dynamics essential to egg activation in zebrafish10.1038/s41598-020-72515-42045-2322https://doaj.org/article/26c9ba675b2d41c7be443925d056b1832020-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-72515-4https://doaj.org/toc/2045-2322Abstract The zinc transporter ZIP9 (SLC39A9) was recently characterized as a membrane androgen receptor in various teleost and mammalian cell models. ZIP9 shows the highest expression in ovaries of teleosts, a tissue in which both androgen signaling and zinc dynamics have significant roles. To examine the role of ZIP9 in ovarian physiology, we generated a ZIP9-mutant zebrafish strain using a CRISPR/Cas9 system. zip9 -/- females showed significant reductions in fecundity, embryo viability, and growth of their offspring compared to wildtype (WT) fish. Furthermore, a high proportion of zip9 -/- eggs failed to undergo normal chorion elevation during activation. In WT eggs, zinc was detected in cortically-localized vesicles which underwent exocytosis upon activation. zip9 -/- eggs showed abnormal cortical vesicle development and had a significantly depressed activation-induced zinc release compared to WT eggs. Moreover, pharmacologically sustained elevation of zinc in WT eggs prior to activation resulted in abnormal chorion elevation similar to that observed in zip9 -/- eggs. These results indicate that ZIP9 is essential for proper zinc modulation during zebrafish egg activation and presents the first evidence of zinc modulation during egg activation in a non-mammalian species.Aubrey ConversePeter ThomasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-14 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aubrey Converse Peter Thomas The zinc transporter ZIP9 (Slc39a9) regulates zinc dynamics essential to egg activation in zebrafish
description	Abstract The zinc transporter ZIP9 (SLC39A9) was recently characterized as a membrane androgen receptor in various teleost and mammalian cell models. ZIP9 shows the highest expression in ovaries of teleosts, a tissue in which both androgen signaling and zinc dynamics have significant roles. To examine the role of ZIP9 in ovarian physiology, we generated a ZIP9-mutant zebrafish strain using a CRISPR/Cas9 system. zip9 -/- females showed significant reductions in fecundity, embryo viability, and growth of their offspring compared to wildtype (WT) fish. Furthermore, a high proportion of zip9 -/- eggs failed to undergo normal chorion elevation during activation. In WT eggs, zinc was detected in cortically-localized vesicles which underwent exocytosis upon activation. zip9 -/- eggs showed abnormal cortical vesicle development and had a significantly depressed activation-induced zinc release compared to WT eggs. Moreover, pharmacologically sustained elevation of zinc in WT eggs prior to activation resulted in abnormal chorion elevation similar to that observed in zip9 -/- eggs. These results indicate that ZIP9 is essential for proper zinc modulation during zebrafish egg activation and presents the first evidence of zinc modulation during egg activation in a non-mammalian species.
format	article
author	Aubrey Converse Peter Thomas
author_facet	Aubrey Converse Peter Thomas
author_sort	Aubrey Converse
title	The zinc transporter ZIP9 (Slc39a9) regulates zinc dynamics essential to egg activation in zebrafish
title_short	The zinc transporter ZIP9 (Slc39a9) regulates zinc dynamics essential to egg activation in zebrafish
title_full	The zinc transporter ZIP9 (Slc39a9) regulates zinc dynamics essential to egg activation in zebrafish
title_fullStr	The zinc transporter ZIP9 (Slc39a9) regulates zinc dynamics essential to egg activation in zebrafish
title_full_unstemmed	The zinc transporter ZIP9 (Slc39a9) regulates zinc dynamics essential to egg activation in zebrafish
title_sort	zinc transporter zip9 (slc39a9) regulates zinc dynamics essential to egg activation in zebrafish
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/26c9ba675b2d41c7be443925d056b183
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The zinc transporter ZIP9 (Slc39a9) regulates zinc dynamics essential to egg activation in zebrafish

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