Circular RNAs in peripheral blood mononuclear cells from ankylosing spondylitis

Abstract. Background:. Circular RNA (circRNA) is a type of closed circular noncoding RNA (ncRNA), mostly formed by back-splicing or alternative splicing of pre-messenger RNA (mRNA). The aim of this study was to explore the expression profile of circRNA in peripheral blood mononuclear cells (PBMCs) o...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yi-Ping Tang, Quan-Bo Zhang, Fei Dai, Xia Liao, Zeng-Rong Dong, Ting Yi, Yu-Feng Qing, Li-Shao Guo
Formato:	article
Lenguaje:	EN
Publicado:	Wolters Kluwer 2021
Materias:	Medicine R
Acceso en línea:	https://doaj.org/article/26cc1161f6e741a29c94a5e1f8002723
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:26cc1161f6e741a29c94a5e1f8002723
record_format	dspace
spelling	oai:doaj.org-article:26cc1161f6e741a29c94a5e1f80027232021-11-25T07:57:20ZCircular RNAs in peripheral blood mononuclear cells from ankylosing spondylitis0366-69992542-564110.1097/CM9.0000000000001815https://doaj.org/article/26cc1161f6e741a29c94a5e1f80027232021-11-01T00:00:00Zhttp://journals.lww.com/10.1097/CM9.0000000000001815https://doaj.org/toc/0366-6999https://doaj.org/toc/2542-5641Abstract. Background:. Circular RNA (circRNA) is a type of closed circular noncoding RNA (ncRNA), mostly formed by back-splicing or alternative splicing of pre-messenger RNA (mRNA). The aim of this study was to explore the expression profile of circRNA in peripheral blood mononuclear cells (PBMCs) of patients with ankylosing spondylitis (AS) and discover potential molecular markers of AS. Methods:. The circRNA microarray technology was used to detect the expression of circRNAs in the peripheral blood of 6 patients with AS and 6 healthy controls (HC). To screen the differentially expressed circRNAs by fold change (FC) and P value, these differentially expressed circRNAs were analyzed by bioinformatics. In 60 cases of AS and 30 cases of HC, 4 circRNAs were subjected to real-time fluorescence quantitative polymerase chain reaction (RT-qPCR), and their correlation with various clinical indicators was analyzed. Finally, the receiver operating characteristic (ROC) curve was used to analyze their potential as AS diagnostic markers. Results:. The microarray results showed that there were 1369 significantly differently expressed (P < 0.05, FC > 1.5) circRNAs between the AS and HC groups (675 upregulated and 694 downregulated). The results of bioinformatics analysis suggested that they were mainly involved in “enzyme binding,” “adenosine ribonucleotide binding,” “MAPK signaling pathway”, etc. The RT-qPCR results showed that the expressions of hsa_circRNA_001544 (U = 486.5, P < 0.05) and hsa_circRNA_102532 (U = 645, P < 0.05) were significantly different between the AS group and the HC group. The AS group was further divided into two subgroups: active AS (ASA) and stable AS (ASS). After analysis, it was found that compared with the HC group, hsa_circRNA_001544 was significantly increased in both ASA (U = 214, P < 0.05) and ASS groups (U = 273, P < 0.05), while hsa_circRNA_008961 (U = 250, P < 0.05) and hsa_circRNA_102532 (U = 295, P < 0.05) were only significantly increased in the ASA group. Furthermore, hsa_circRNA_012732 was significantly different between the ASA and ASS groups (U = 194, P < 0.05), and there was no statistical significance among the remaining groups. Correlation analysis results showed that hsa_circRNA_012732 was negatively correlated with Bath Ankylosing Spondylitis Disease Activity Index (BASDAI), high-sensitivity C-reactive protein (hsCRP), and globulin (GLOB) and positively correlated with lymphocyte count (LY), mean corpusular volume, and albumin (ALB), and hsa_circRNA_008961 was negatively correlated with platelet (PLT) count. ROC curve analysis showed that hsa_circRNA_001544 (95% CI = 0.610–0.831, P < 0.05) and hsa_circRNA_102532 (95% CI = 0.521–0.762, P < 0.05) were statistically significant, and their area under curve (AUC) values were 0.720 and 0.642, respectively. Conclusions:. There are differentially expressed circRNAs in PBMCs of AS patients, and they may be involved in the occurrence and development of AS. Among these differentially expressed circRNAs, hsa_circRNA_012732 has the potential to become an indicator of disease activity, and hsa_circRNA_001544 has the potential to become a molecular marker for AS diagnosis.Yi-Ping TangQuan-Bo ZhangFei DaiXia LiaoZeng-Rong DongTing YiYu-Feng QingLi-Shao GuoWolters KluwerarticleMedicineRENChinese Medical Journal, Vol 134, Iss 21, Pp 2573-2582 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R
spellingShingle	Medicine R Yi-Ping Tang Quan-Bo Zhang Fei Dai Xia Liao Zeng-Rong Dong Ting Yi Yu-Feng Qing Li-Shao Guo Circular RNAs in peripheral blood mononuclear cells from ankylosing spondylitis
description	Abstract. Background:. Circular RNA (circRNA) is a type of closed circular noncoding RNA (ncRNA), mostly formed by back-splicing or alternative splicing of pre-messenger RNA (mRNA). The aim of this study was to explore the expression profile of circRNA in peripheral blood mononuclear cells (PBMCs) of patients with ankylosing spondylitis (AS) and discover potential molecular markers of AS. Methods:. The circRNA microarray technology was used to detect the expression of circRNAs in the peripheral blood of 6 patients with AS and 6 healthy controls (HC). To screen the differentially expressed circRNAs by fold change (FC) and P value, these differentially expressed circRNAs were analyzed by bioinformatics. In 60 cases of AS and 30 cases of HC, 4 circRNAs were subjected to real-time fluorescence quantitative polymerase chain reaction (RT-qPCR), and their correlation with various clinical indicators was analyzed. Finally, the receiver operating characteristic (ROC) curve was used to analyze their potential as AS diagnostic markers. Results:. The microarray results showed that there were 1369 significantly differently expressed (P < 0.05, FC > 1.5) circRNAs between the AS and HC groups (675 upregulated and 694 downregulated). The results of bioinformatics analysis suggested that they were mainly involved in “enzyme binding,” “adenosine ribonucleotide binding,” “MAPK signaling pathway”, etc. The RT-qPCR results showed that the expressions of hsa_circRNA_001544 (U = 486.5, P < 0.05) and hsa_circRNA_102532 (U = 645, P < 0.05) were significantly different between the AS group and the HC group. The AS group was further divided into two subgroups: active AS (ASA) and stable AS (ASS). After analysis, it was found that compared with the HC group, hsa_circRNA_001544 was significantly increased in both ASA (U = 214, P < 0.05) and ASS groups (U = 273, P < 0.05), while hsa_circRNA_008961 (U = 250, P < 0.05) and hsa_circRNA_102532 (U = 295, P < 0.05) were only significantly increased in the ASA group. Furthermore, hsa_circRNA_012732 was significantly different between the ASA and ASS groups (U = 194, P < 0.05), and there was no statistical significance among the remaining groups. Correlation analysis results showed that hsa_circRNA_012732 was negatively correlated with Bath Ankylosing Spondylitis Disease Activity Index (BASDAI), high-sensitivity C-reactive protein (hsCRP), and globulin (GLOB) and positively correlated with lymphocyte count (LY), mean corpusular volume, and albumin (ALB), and hsa_circRNA_008961 was negatively correlated with platelet (PLT) count. ROC curve analysis showed that hsa_circRNA_001544 (95% CI = 0.610–0.831, P < 0.05) and hsa_circRNA_102532 (95% CI = 0.521–0.762, P < 0.05) were statistically significant, and their area under curve (AUC) values were 0.720 and 0.642, respectively. Conclusions:. There are differentially expressed circRNAs in PBMCs of AS patients, and they may be involved in the occurrence and development of AS. Among these differentially expressed circRNAs, hsa_circRNA_012732 has the potential to become an indicator of disease activity, and hsa_circRNA_001544 has the potential to become a molecular marker for AS diagnosis.
format	article
author	Yi-Ping Tang Quan-Bo Zhang Fei Dai Xia Liao Zeng-Rong Dong Ting Yi Yu-Feng Qing Li-Shao Guo
author_facet	Yi-Ping Tang Quan-Bo Zhang Fei Dai Xia Liao Zeng-Rong Dong Ting Yi Yu-Feng Qing Li-Shao Guo
author_sort	Yi-Ping Tang
title	Circular RNAs in peripheral blood mononuclear cells from ankylosing spondylitis
title_short	Circular RNAs in peripheral blood mononuclear cells from ankylosing spondylitis
title_full	Circular RNAs in peripheral blood mononuclear cells from ankylosing spondylitis
title_fullStr	Circular RNAs in peripheral blood mononuclear cells from ankylosing spondylitis
title_full_unstemmed	Circular RNAs in peripheral blood mononuclear cells from ankylosing spondylitis
title_sort	circular rnas in peripheral blood mononuclear cells from ankylosing spondylitis
publisher	Wolters Kluwer
publishDate	2021
url	https://doaj.org/article/26cc1161f6e741a29c94a5e1f8002723
work_keys_str_mv	AT yipingtang circularrnasinperipheralbloodmononuclearcellsfromankylosingspondylitis AT quanbozhang circularrnasinperipheralbloodmononuclearcellsfromankylosingspondylitis AT feidai circularrnasinperipheralbloodmononuclearcellsfromankylosingspondylitis AT xialiao circularrnasinperipheralbloodmononuclearcellsfromankylosingspondylitis AT zengrongdong circularrnasinperipheralbloodmononuclearcellsfromankylosingspondylitis AT tingyi circularrnasinperipheralbloodmononuclearcellsfromankylosingspondylitis AT yufengqing circularrnasinperipheralbloodmononuclearcellsfromankylosingspondylitis AT lishaoguo circularrnasinperipheralbloodmononuclearcellsfromankylosingspondylitis
_version_	1718413606395052032

Circular RNAs in peripheral blood mononuclear cells from ankylosing spondylitis

Ejemplares similares