Mucosal IFNγ production and potential role in protection in Escherichia coli O157:H7 vaccinated and challenged cattle

Abstract Shiga-toxin producing Escherichia coli O157:H7 (O157)-based vaccines can provide a potential intervention strategy to limit foodborne zoonotic transmission of O157. While the peripheral antibody response to O157 vaccination has been characterized, O157-specific cellular immunity at the rect...

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Autores principales:	Robert G. Schaut, Mitchell V. Palmer, Paola M. Boggiatto, Indira T. Kudva, Crystal L. Loving, Vijay K. Sharma
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/26d3417dc4354d8bb99347b10cc41818
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spelling	oai:doaj.org-article:26d3417dc4354d8bb99347b10cc418182021-12-02T14:29:04ZMucosal IFNγ production and potential role in protection in Escherichia coli O157:H7 vaccinated and challenged cattle10.1038/s41598-021-89113-72045-2322https://doaj.org/article/26d3417dc4354d8bb99347b10cc418182021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89113-7https://doaj.org/toc/2045-2322Abstract Shiga-toxin producing Escherichia coli O157:H7 (O157)-based vaccines can provide a potential intervention strategy to limit foodborne zoonotic transmission of O157. While the peripheral antibody response to O157 vaccination has been characterized, O157-specific cellular immunity at the rectoanal junction (RAJ), a preferred site for O157 colonization, remains poorly described. Vaccine induced mucosal O157-specific antibodies likely provide some protection, cellular immune responses at the RAJ may also play a role in protection. Distinct lymphoid follicles were increased in the RAJ of vaccinated/challenged animals. Additionally, increased numbers of interferon (IFN)γ-producing cells and γδ + T cells were detected in the follicular region of the RAJ of vaccinated/challenged animals. Likewise, adjuvanted-vaccine formulation is critical in immunogenicity of the O157 parenteral vaccine. Local T cell produced IFNγ may impact epithelial cells, subsequently limiting O157 adherence, which was demonstrated using in vitro attachment assays with bovine epithelial cells. Thus, distinct immune changes induced at the mucosa of vaccinated and challenged animals provide insight of mechanisms associated with limiting O157 fecal shedding. Enhancing mucosal immunity may be critical in the further development of efficacious vaccines for controlling O157 in ruminants and thus limiting O157 transmission to humans.Robert G. SchautMitchell V. PalmerPaola M. BoggiattoIndira T. KudvaCrystal L. LovingVijay K. SharmaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Robert G. Schaut Mitchell V. Palmer Paola M. Boggiatto Indira T. Kudva Crystal L. Loving Vijay K. Sharma Mucosal IFNγ production and potential role in protection in Escherichia coli O157:H7 vaccinated and challenged cattle
description	Abstract Shiga-toxin producing Escherichia coli O157:H7 (O157)-based vaccines can provide a potential intervention strategy to limit foodborne zoonotic transmission of O157. While the peripheral antibody response to O157 vaccination has been characterized, O157-specific cellular immunity at the rectoanal junction (RAJ), a preferred site for O157 colonization, remains poorly described. Vaccine induced mucosal O157-specific antibodies likely provide some protection, cellular immune responses at the RAJ may also play a role in protection. Distinct lymphoid follicles were increased in the RAJ of vaccinated/challenged animals. Additionally, increased numbers of interferon (IFN)γ-producing cells and γδ + T cells were detected in the follicular region of the RAJ of vaccinated/challenged animals. Likewise, adjuvanted-vaccine formulation is critical in immunogenicity of the O157 parenteral vaccine. Local T cell produced IFNγ may impact epithelial cells, subsequently limiting O157 adherence, which was demonstrated using in vitro attachment assays with bovine epithelial cells. Thus, distinct immune changes induced at the mucosa of vaccinated and challenged animals provide insight of mechanisms associated with limiting O157 fecal shedding. Enhancing mucosal immunity may be critical in the further development of efficacious vaccines for controlling O157 in ruminants and thus limiting O157 transmission to humans.
format	article
author	Robert G. Schaut Mitchell V. Palmer Paola M. Boggiatto Indira T. Kudva Crystal L. Loving Vijay K. Sharma
author_facet	Robert G. Schaut Mitchell V. Palmer Paola M. Boggiatto Indira T. Kudva Crystal L. Loving Vijay K. Sharma
author_sort	Robert G. Schaut
title	Mucosal IFNγ production and potential role in protection in Escherichia coli O157:H7 vaccinated and challenged cattle
title_short	Mucosal IFNγ production and potential role in protection in Escherichia coli O157:H7 vaccinated and challenged cattle
title_full	Mucosal IFNγ production and potential role in protection in Escherichia coli O157:H7 vaccinated and challenged cattle
title_fullStr	Mucosal IFNγ production and potential role in protection in Escherichia coli O157:H7 vaccinated and challenged cattle
title_full_unstemmed	Mucosal IFNγ production and potential role in protection in Escherichia coli O157:H7 vaccinated and challenged cattle
title_sort	mucosal ifnγ production and potential role in protection in escherichia coli o157:h7 vaccinated and challenged cattle
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/26d3417dc4354d8bb99347b10cc41818
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Mucosal IFNγ production and potential role in protection in Escherichia coli O157:H7 vaccinated and challenged cattle

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