Distribution of salicifoline in freeze-fixed stems of Magnolia kobus as observed by cryo-TOF-SIMS

Abstract Alkaloids are basic nitrogen-containing chemicals that have important physiological and pharmacological characteristics. Many vascular plant species contain alkaloids, and their roles in planta are of interest. However, the detailed distribution of alkaloids remains unclear because of their...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wakaba Okumura, Dan Aoki, Yasuyuki Matsushita, Masato Yoshida, Kazuhiko Fukushima
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/26d43cd941894deb8ec2adb552b2a3ad
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:26d43cd941894deb8ec2adb552b2a3ad
record_format	dspace
spelling	oai:doaj.org-article:26d43cd941894deb8ec2adb552b2a3ad2021-12-02T12:30:37ZDistribution of salicifoline in freeze-fixed stems of Magnolia kobus as observed by cryo-TOF-SIMS10.1038/s41598-017-06444-02045-2322https://doaj.org/article/26d43cd941894deb8ec2adb552b2a3ad2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06444-0https://doaj.org/toc/2045-2322Abstract Alkaloids are basic nitrogen-containing chemicals that have important physiological and pharmacological characteristics. Many vascular plant species contain alkaloids, and their roles in planta are of interest. However, the detailed distribution of alkaloids remains unclear because of their low water solubility and low concentrations in plants. In this study, we visualized the distribution of salicifoline, a water-soluble quaternary ammonium alkaloid, in the freeze-fixed stems of Magnolia kobus by cryo time-of-flight secondary ion mass spectrometry. Most of the salicifoline was distributed in living phloem tissues. In the xylem, salicifoline was detected in ray cells, lignifying wood fibres, and in vessels in the latest annual ring. The salicifoline distribution in the xylem varied with the cell wall formation stage. These results provide new insights into the storage, transportation, and role of the alkaloid salicifoline in M. kobus.Wakaba OkumuraDan AokiYasuyuki MatsushitaMasato YoshidaKazuhiko FukushimaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wakaba Okumura Dan Aoki Yasuyuki Matsushita Masato Yoshida Kazuhiko Fukushima Distribution of salicifoline in freeze-fixed stems of Magnolia kobus as observed by cryo-TOF-SIMS
description	Abstract Alkaloids are basic nitrogen-containing chemicals that have important physiological and pharmacological characteristics. Many vascular plant species contain alkaloids, and their roles in planta are of interest. However, the detailed distribution of alkaloids remains unclear because of their low water solubility and low concentrations in plants. In this study, we visualized the distribution of salicifoline, a water-soluble quaternary ammonium alkaloid, in the freeze-fixed stems of Magnolia kobus by cryo time-of-flight secondary ion mass spectrometry. Most of the salicifoline was distributed in living phloem tissues. In the xylem, salicifoline was detected in ray cells, lignifying wood fibres, and in vessels in the latest annual ring. The salicifoline distribution in the xylem varied with the cell wall formation stage. These results provide new insights into the storage, transportation, and role of the alkaloid salicifoline in M. kobus.
format	article
author	Wakaba Okumura Dan Aoki Yasuyuki Matsushita Masato Yoshida Kazuhiko Fukushima
author_facet	Wakaba Okumura Dan Aoki Yasuyuki Matsushita Masato Yoshida Kazuhiko Fukushima
author_sort	Wakaba Okumura
title	Distribution of salicifoline in freeze-fixed stems of Magnolia kobus as observed by cryo-TOF-SIMS
title_short	Distribution of salicifoline in freeze-fixed stems of Magnolia kobus as observed by cryo-TOF-SIMS
title_full	Distribution of salicifoline in freeze-fixed stems of Magnolia kobus as observed by cryo-TOF-SIMS
title_fullStr	Distribution of salicifoline in freeze-fixed stems of Magnolia kobus as observed by cryo-TOF-SIMS
title_full_unstemmed	Distribution of salicifoline in freeze-fixed stems of Magnolia kobus as observed by cryo-TOF-SIMS
title_sort	distribution of salicifoline in freeze-fixed stems of magnolia kobus as observed by cryo-tof-sims
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/26d43cd941894deb8ec2adb552b2a3ad
work_keys_str_mv	AT wakabaokumura distributionofsalicifolineinfreezefixedstemsofmagnoliakobusasobservedbycryotofsims AT danaoki distributionofsalicifolineinfreezefixedstemsofmagnoliakobusasobservedbycryotofsims AT yasuyukimatsushita distributionofsalicifolineinfreezefixedstemsofmagnoliakobusasobservedbycryotofsims AT masatoyoshida distributionofsalicifolineinfreezefixedstemsofmagnoliakobusasobservedbycryotofsims AT kazuhikofukushima distributionofsalicifolineinfreezefixedstemsofmagnoliakobusasobservedbycryotofsims
_version_	1718394323693731840

Distribution of salicifoline in freeze-fixed stems of Magnolia kobus as observed by cryo-TOF-SIMS

Ejemplares similares