The Humoral Immune Response to BNT162b2 Vaccine Is Associated With Circulating CD19+ B Lymphocytes and the Naïve CD45RA to Memory CD45RO CD4+ T Helper Cells Ratio in Hemodialysis Patients and Kidney Transplant Recipients
BackgroundThe humoral and cellular immune responses to SARS-COV-2 vaccination remain to be elucidated in hemodialysis (HD) patients and kidney transplant recipients (KTRs), considering their baseline immunosuppressed status. The aim of our study was to assess the associations of vaccine-induced anti...
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oai:doaj.org-article:26df9da6532049188a5616ea0192b4822021-12-03T06:49:11ZThe Humoral Immune Response to BNT162b2 Vaccine Is Associated With Circulating CD19+ B Lymphocytes and the Naïve CD45RA to Memory CD45RO CD4+ T Helper Cells Ratio in Hemodialysis Patients and Kidney Transplant Recipients1664-322410.3389/fimmu.2021.760249https://doaj.org/article/26df9da6532049188a5616ea0192b4822021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.760249/fullhttps://doaj.org/toc/1664-3224BackgroundThe humoral and cellular immune responses to SARS-COV-2 vaccination remain to be elucidated in hemodialysis (HD) patients and kidney transplant recipients (KTRs), considering their baseline immunosuppressed status. The aim of our study was to assess the associations of vaccine-induced antibody responses with circulating lymphocytes sub-populations and their respective patterns of alterations in maintenance HD patients and KTRs.Materials and MethodsWe included 34 HD patients and 54 KTRs who received two doses of the mRNA-vaccine BNT162b2. Lymphocyte subpopulations were analyzed by flow cytometry before vaccination (T0), before the second vaccine dose (T1) and 2 weeks after the second dose (T2). The anti-SARS-CoV2 antibody response was assessed at T1 and at T2.Results31 HD patients (91.8%) and 16 KTRs (29.6%) became seropositive at T2. HD patients who became seropositive following the first dose displayed higher CD19+ B lymphocytes compared to their seronegative HD counterparts. A positive correlation was established between CD19+ B cells counts and antibody titers at all time-points in both groups (p < 0.001). KTRs showed higher naïve CD4+CD45RA+ T helper cells compared to HD patients at baseline and T2 whereas HD patients displayed higher memory CD45RO+ T cells compared to KTRs at T2. The naïve CD4+CD45RA to memory CD4+CD45RO+ T helper cells fraction was negatively associated with antibody production in both groups.ConclusionsOur study provides a potential conceptual framework for monitoring vaccination efficacy in HD patients and KTRs considering the correlation established between CD19+ B cells, generation of memory CD4+ T helper cells and anti SARS-CoV2 antibody response to vaccination.Anila DuniAnila DuniGeorgios S. MarkopoulosIoannis MalliorasHaralampos PappasHaralampos PappasEfthymios PappasVasileios KoutlasEirini TzalavraGerasimos BaxevanosGerasimos BaxevanosSilvia PriskaKonstantina GartzonikaMichael MitsisEvangelia DounousiEvangelia DounousiEvangelia DounousiFrontiers Media S.A.articleSARS-COV-2 vaccinationhemodialysiskidney transplant recipientsanti-SARS-CoV2 antibodiesCD19+ B lymphocytesnaïve CD4+CD45RA+ T helper cellsImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021) |
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DOAJ |
| collection |
DOAJ |
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EN |
| topic |
SARS-COV-2 vaccination hemodialysis kidney transplant recipients anti-SARS-CoV2 antibodies CD19+ B lymphocytes naïve CD4+CD45RA+ T helper cells Immunologic diseases. Allergy RC581-607 |
| spellingShingle |
SARS-COV-2 vaccination hemodialysis kidney transplant recipients anti-SARS-CoV2 antibodies CD19+ B lymphocytes naïve CD4+CD45RA+ T helper cells Immunologic diseases. Allergy RC581-607 Anila Duni Anila Duni Georgios S. Markopoulos Ioannis Mallioras Haralampos Pappas Haralampos Pappas Efthymios Pappas Vasileios Koutlas Eirini Tzalavra Gerasimos Baxevanos Gerasimos Baxevanos Silvia Priska Konstantina Gartzonika Michael Mitsis Evangelia Dounousi Evangelia Dounousi Evangelia Dounousi The Humoral Immune Response to BNT162b2 Vaccine Is Associated With Circulating CD19+ B Lymphocytes and the Naïve CD45RA to Memory CD45RO CD4+ T Helper Cells Ratio in Hemodialysis Patients and Kidney Transplant Recipients |
| description |
BackgroundThe humoral and cellular immune responses to SARS-COV-2 vaccination remain to be elucidated in hemodialysis (HD) patients and kidney transplant recipients (KTRs), considering their baseline immunosuppressed status. The aim of our study was to assess the associations of vaccine-induced antibody responses with circulating lymphocytes sub-populations and their respective patterns of alterations in maintenance HD patients and KTRs.Materials and MethodsWe included 34 HD patients and 54 KTRs who received two doses of the mRNA-vaccine BNT162b2. Lymphocyte subpopulations were analyzed by flow cytometry before vaccination (T0), before the second vaccine dose (T1) and 2 weeks after the second dose (T2). The anti-SARS-CoV2 antibody response was assessed at T1 and at T2.Results31 HD patients (91.8%) and 16 KTRs (29.6%) became seropositive at T2. HD patients who became seropositive following the first dose displayed higher CD19+ B lymphocytes compared to their seronegative HD counterparts. A positive correlation was established between CD19+ B cells counts and antibody titers at all time-points in both groups (p < 0.001). KTRs showed higher naïve CD4+CD45RA+ T helper cells compared to HD patients at baseline and T2 whereas HD patients displayed higher memory CD45RO+ T cells compared to KTRs at T2. The naïve CD4+CD45RA to memory CD4+CD45RO+ T helper cells fraction was negatively associated with antibody production in both groups.ConclusionsOur study provides a potential conceptual framework for monitoring vaccination efficacy in HD patients and KTRs considering the correlation established between CD19+ B cells, generation of memory CD4+ T helper cells and anti SARS-CoV2 antibody response to vaccination. |
| format |
article |
| author |
Anila Duni Anila Duni Georgios S. Markopoulos Ioannis Mallioras Haralampos Pappas Haralampos Pappas Efthymios Pappas Vasileios Koutlas Eirini Tzalavra Gerasimos Baxevanos Gerasimos Baxevanos Silvia Priska Konstantina Gartzonika Michael Mitsis Evangelia Dounousi Evangelia Dounousi Evangelia Dounousi |
| author_facet |
Anila Duni Anila Duni Georgios S. Markopoulos Ioannis Mallioras Haralampos Pappas Haralampos Pappas Efthymios Pappas Vasileios Koutlas Eirini Tzalavra Gerasimos Baxevanos Gerasimos Baxevanos Silvia Priska Konstantina Gartzonika Michael Mitsis Evangelia Dounousi Evangelia Dounousi Evangelia Dounousi |
| author_sort |
Anila Duni |
| title |
The Humoral Immune Response to BNT162b2 Vaccine Is Associated With Circulating CD19+ B Lymphocytes and the Naïve CD45RA to Memory CD45RO CD4+ T Helper Cells Ratio in Hemodialysis Patients and Kidney Transplant Recipients |
| title_short |
The Humoral Immune Response to BNT162b2 Vaccine Is Associated With Circulating CD19+ B Lymphocytes and the Naïve CD45RA to Memory CD45RO CD4+ T Helper Cells Ratio in Hemodialysis Patients and Kidney Transplant Recipients |
| title_full |
The Humoral Immune Response to BNT162b2 Vaccine Is Associated With Circulating CD19+ B Lymphocytes and the Naïve CD45RA to Memory CD45RO CD4+ T Helper Cells Ratio in Hemodialysis Patients and Kidney Transplant Recipients |
| title_fullStr |
The Humoral Immune Response to BNT162b2 Vaccine Is Associated With Circulating CD19+ B Lymphocytes and the Naïve CD45RA to Memory CD45RO CD4+ T Helper Cells Ratio in Hemodialysis Patients and Kidney Transplant Recipients |
| title_full_unstemmed |
The Humoral Immune Response to BNT162b2 Vaccine Is Associated With Circulating CD19+ B Lymphocytes and the Naïve CD45RA to Memory CD45RO CD4+ T Helper Cells Ratio in Hemodialysis Patients and Kidney Transplant Recipients |
| title_sort |
humoral immune response to bnt162b2 vaccine is associated with circulating cd19+ b lymphocytes and the naïve cd45ra to memory cd45ro cd4+ t helper cells ratio in hemodialysis patients and kidney transplant recipients |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/26df9da6532049188a5616ea0192b482 |
| work_keys_str_mv |
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