Aβ1–40-Induced Platelet Adhesion Is Ameliorated by Rosmarinic Acid through Inhibition of NADPH Oxidase/PKC-δ/Integrin αIIbβ3 Signaling

In platelets, oxidative stress reportedly increases platelet adhesion to vessels, thus promoting the vascular pathology of various neurodegenerative diseases, including Alzheimer’s disease (AD). Recently, it has been shown that β-amyloid (Aβ) can increase oxidative stress in platelets; however, the...

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Autores principales:	Bo Kyung Lee, Hye Jin Jee, Yi-Sook Jung
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	platelet rosmarinic acid (RA) β-amyloid (Aβ) NADPH oxidase integrin α<sub>IIb</sub>β<sub>3</sub> Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/26ea9b04d8714705bc2a1daa0ac45495
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spelling	oai:doaj.org-article:26ea9b04d8714705bc2a1daa0ac454952021-11-25T16:25:45ZAβ<sub>1–40</sub>-Induced Platelet Adhesion Is Ameliorated by Rosmarinic Acid through Inhibition of NADPH Oxidase/PKC-δ/Integrin α<sub>IIb</sub>β<sub>3</sub> Signaling10.3390/antiox101116712076-3921https://doaj.org/article/26ea9b04d8714705bc2a1daa0ac454952021-10-01T00:00:00Zhttps://www.mdpi.com/2076-3921/10/11/1671https://doaj.org/toc/2076-3921In platelets, oxidative stress reportedly increases platelet adhesion to vessels, thus promoting the vascular pathology of various neurodegenerative diseases, including Alzheimer’s disease (AD). Recently, it has been shown that β-amyloid (Aβ) can increase oxidative stress in platelets; however, the underlying mechanism remains elusive. In the present study, we aimed to elucidate the signaling pathway of platelet adhesion induced by Aβ<sub>1–40</sub>, the major form of circulating Aβ, through Western blotting, immunofluorescence confocal microscopy, and fluorescence-activated cell sorting analysis. Additionally, we examined whether rosmarinic acid (RA), a natural polyphenol antioxidant, can modulate these processes. Our results show that Aβ<sub>1–40</sub>-induced platelet adhesion is mediated through NADPH oxidase/ROS/PKC-δ/integrin α<sub>IIb</sub>β<sub>3</sub> signaling, and these signaling pathways are significantly inhibited by RA. Collectively, these results suggest that RA may have beneficial effects on platelet-associated vascular pathology in AD.Bo Kyung LeeHye Jin JeeYi-Sook JungMDPI AGarticleplateletrosmarinic acid (RA)β-amyloid (Aβ)NADPH oxidaseintegrin α<sub>IIb</sub>β<sub>3</sub>Therapeutics. PharmacologyRM1-950ENAntioxidants, Vol 10, Iss 1671, p 1671 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	platelet rosmarinic acid (RA) β-amyloid (Aβ) NADPH oxidase integrin α<sub>IIb</sub>β<sub>3</sub> Therapeutics. Pharmacology RM1-950
spellingShingle	platelet rosmarinic acid (RA) β-amyloid (Aβ) NADPH oxidase integrin α<sub>IIb</sub>β<sub>3</sub> Therapeutics. Pharmacology RM1-950 Bo Kyung Lee Hye Jin Jee Yi-Sook Jung Aβ<sub>1–40</sub>-Induced Platelet Adhesion Is Ameliorated by Rosmarinic Acid through Inhibition of NADPH Oxidase/PKC-δ/Integrin α<sub>IIb</sub>β<sub>3</sub> Signaling
description	In platelets, oxidative stress reportedly increases platelet adhesion to vessels, thus promoting the vascular pathology of various neurodegenerative diseases, including Alzheimer’s disease (AD). Recently, it has been shown that β-amyloid (Aβ) can increase oxidative stress in platelets; however, the underlying mechanism remains elusive. In the present study, we aimed to elucidate the signaling pathway of platelet adhesion induced by Aβ<sub>1–40</sub>, the major form of circulating Aβ, through Western blotting, immunofluorescence confocal microscopy, and fluorescence-activated cell sorting analysis. Additionally, we examined whether rosmarinic acid (RA), a natural polyphenol antioxidant, can modulate these processes. Our results show that Aβ<sub>1–40</sub>-induced platelet adhesion is mediated through NADPH oxidase/ROS/PKC-δ/integrin α<sub>IIb</sub>β<sub>3</sub> signaling, and these signaling pathways are significantly inhibited by RA. Collectively, these results suggest that RA may have beneficial effects on platelet-associated vascular pathology in AD.
format	article
author	Bo Kyung Lee Hye Jin Jee Yi-Sook Jung
author_facet	Bo Kyung Lee Hye Jin Jee Yi-Sook Jung
author_sort	Bo Kyung Lee
title	Aβ<sub>1–40</sub>-Induced Platelet Adhesion Is Ameliorated by Rosmarinic Acid through Inhibition of NADPH Oxidase/PKC-δ/Integrin α<sub>IIb</sub>β<sub>3</sub> Signaling
title_short	Aβ<sub>1–40</sub>-Induced Platelet Adhesion Is Ameliorated by Rosmarinic Acid through Inhibition of NADPH Oxidase/PKC-δ/Integrin α<sub>IIb</sub>β<sub>3</sub> Signaling
title_full	Aβ<sub>1–40</sub>-Induced Platelet Adhesion Is Ameliorated by Rosmarinic Acid through Inhibition of NADPH Oxidase/PKC-δ/Integrin α<sub>IIb</sub>β<sub>3</sub> Signaling
title_fullStr	Aβ<sub>1–40</sub>-Induced Platelet Adhesion Is Ameliorated by Rosmarinic Acid through Inhibition of NADPH Oxidase/PKC-δ/Integrin α<sub>IIb</sub>β<sub>3</sub> Signaling
title_full_unstemmed	Aβ<sub>1–40</sub>-Induced Platelet Adhesion Is Ameliorated by Rosmarinic Acid through Inhibition of NADPH Oxidase/PKC-δ/Integrin α<sub>IIb</sub>β<sub>3</sub> Signaling
title_sort	aβ<sub>1–40</sub>-induced platelet adhesion is ameliorated by rosmarinic acid through inhibition of nadph oxidase/pkc-δ/integrin α<sub>iib</sub>β<sub>3</sub> signaling
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/26ea9b04d8714705bc2a1daa0ac45495
work_keys_str_mv	AT bokyunglee absub140subinducedplateletadhesionisamelioratedbyrosmarinicacidthroughinhibitionofnadphoxidasepkcdintegrinasubiibsubbsub3subsignaling AT hyejinjee absub140subinducedplateletadhesionisamelioratedbyrosmarinicacidthroughinhibitionofnadphoxidasepkcdintegrinasubiibsubbsub3subsignaling AT yisookjung absub140subinducedplateletadhesionisamelioratedbyrosmarinicacidthroughinhibitionofnadphoxidasepkcdintegrinasubiibsubbsub3subsignaling
_version_	1718413188409589760

Aβ<sub>1–40</sub>-Induced Platelet Adhesion Is Ameliorated by Rosmarinic Acid through Inhibition of NADPH Oxidase/PKC-δ/Integrin α<sub>IIb</sub>β<sub>3</sub> Signaling

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