MicroRNA-378a-3p is overexpressed in psoriasis and modulates cell cycle arrest in keratinocytes via targeting BMP2 gene

Abstract Psoriasis is a chronic autoimmune skin disease driven by dysregulations at the cellular, genomic and genetic levels. MicroRNAs are key mediators of gene expression regulation. However, how microRNAs control the pathogenesis of psoriasis is still unclear. Here, we reported a significant up-r...

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Autores principales:	Wipasiri Soonthornchai, Pattarin Tangtanatakul, Kornvalee Meesilpavikkai, Virgil Dalm, Patipark Kueanjinda, Jongkonnee Wongpiyabovorn
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/26efc8aafcf94809bc85f58eff36ea7d
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spelling	oai:doaj.org-article:26efc8aafcf94809bc85f58eff36ea7d2021-12-02T15:22:57ZMicroRNA-378a-3p is overexpressed in psoriasis and modulates cell cycle arrest in keratinocytes via targeting BMP2 gene10.1038/s41598-021-93616-82045-2322https://doaj.org/article/26efc8aafcf94809bc85f58eff36ea7d2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93616-8https://doaj.org/toc/2045-2322Abstract Psoriasis is a chronic autoimmune skin disease driven by dysregulations at the cellular, genomic and genetic levels. MicroRNAs are key mediators of gene expression regulation. However, how microRNAs control the pathogenesis of psoriasis is still unclear. Here, we reported a significant up-regulation of miR-378a-3p (miR-378a) in skin biopsies from active psoriatic lesions while it was down-regulated after treatment with methotrexate or narrow-band ultraviolet B phototherapy. Using the keratinocyte in vitro model, we showed that miR-378a disturbed the cell cycle progression, causing cell cycle arrest at G1 phase. Transcriptomic analysis of keratinocytes with miR-378a overexpression and depletion revealed several important biological mechanisms related to inflammation and tight junction. Target mRNA transcript assessed by luciferase assay identified bone morphogenetic protein 2 as a novel target gene of miR-378a. These findings offer a mechanistic model where miR-378a contributes to the pathogenesis of psoriasis.Wipasiri SoonthornchaiPattarin TangtanatakulKornvalee MeesilpavikkaiVirgil DalmPatipark KueanjindaJongkonnee WongpiyabovornNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wipasiri Soonthornchai Pattarin Tangtanatakul Kornvalee Meesilpavikkai Virgil Dalm Patipark Kueanjinda Jongkonnee Wongpiyabovorn MicroRNA-378a-3p is overexpressed in psoriasis and modulates cell cycle arrest in keratinocytes via targeting BMP2 gene
description	Abstract Psoriasis is a chronic autoimmune skin disease driven by dysregulations at the cellular, genomic and genetic levels. MicroRNAs are key mediators of gene expression regulation. However, how microRNAs control the pathogenesis of psoriasis is still unclear. Here, we reported a significant up-regulation of miR-378a-3p (miR-378a) in skin biopsies from active psoriatic lesions while it was down-regulated after treatment with methotrexate or narrow-band ultraviolet B phototherapy. Using the keratinocyte in vitro model, we showed that miR-378a disturbed the cell cycle progression, causing cell cycle arrest at G1 phase. Transcriptomic analysis of keratinocytes with miR-378a overexpression and depletion revealed several important biological mechanisms related to inflammation and tight junction. Target mRNA transcript assessed by luciferase assay identified bone morphogenetic protein 2 as a novel target gene of miR-378a. These findings offer a mechanistic model where miR-378a contributes to the pathogenesis of psoriasis.
format	article
author	Wipasiri Soonthornchai Pattarin Tangtanatakul Kornvalee Meesilpavikkai Virgil Dalm Patipark Kueanjinda Jongkonnee Wongpiyabovorn
author_facet	Wipasiri Soonthornchai Pattarin Tangtanatakul Kornvalee Meesilpavikkai Virgil Dalm Patipark Kueanjinda Jongkonnee Wongpiyabovorn
author_sort	Wipasiri Soonthornchai
title	MicroRNA-378a-3p is overexpressed in psoriasis and modulates cell cycle arrest in keratinocytes via targeting BMP2 gene
title_short	MicroRNA-378a-3p is overexpressed in psoriasis and modulates cell cycle arrest in keratinocytes via targeting BMP2 gene
title_full	MicroRNA-378a-3p is overexpressed in psoriasis and modulates cell cycle arrest in keratinocytes via targeting BMP2 gene
title_fullStr	MicroRNA-378a-3p is overexpressed in psoriasis and modulates cell cycle arrest in keratinocytes via targeting BMP2 gene
title_full_unstemmed	MicroRNA-378a-3p is overexpressed in psoriasis and modulates cell cycle arrest in keratinocytes via targeting BMP2 gene
title_sort	microrna-378a-3p is overexpressed in psoriasis and modulates cell cycle arrest in keratinocytes via targeting bmp2 gene
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/26efc8aafcf94809bc85f58eff36ea7d
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MicroRNA-378a-3p is overexpressed in psoriasis and modulates cell cycle arrest in keratinocytes via targeting BMP2 gene

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