Evaluation of the Differences in the Expression of Biogenic Amine-Related mRNAs and Proteins in Endometrioid Endometrial Cancer

Biogenic amines, such as adrenaline, noradrenaline, histamine, dopamine, and serotonin are important neurotransmitters that also regulate cell viability. Their detection and analysis are helpful in the diagnosis of many diseases, including cancer. The aim of this study was to determine the expressio...

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Autores principales:	Michał Czerwiński, Anna Bednarska-Czerwińska, Nikola Zmarzły, Dariusz Boroń, Marcin Oplawski, Beniamin Oskar Grabarek
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	endometrial cancer biogenic amines histamine receptors dopamine receptors expression profile Medicine R
Acceso en línea:	https://doaj.org/article/26fab4487c9644348bd0fc1dd5b9d71e
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id	oai:doaj.org-article:26fab4487c9644348bd0fc1dd5b9d71e
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spelling	oai:doaj.org-article:26fab4487c9644348bd0fc1dd5b9d71e2021-11-11T17:31:56ZEvaluation of the Differences in the Expression of Biogenic Amine-Related mRNAs and Proteins in Endometrioid Endometrial Cancer10.3390/jcm102148722077-0383https://doaj.org/article/26fab4487c9644348bd0fc1dd5b9d71e2021-10-01T00:00:00Zhttps://www.mdpi.com/2077-0383/10/21/4872https://doaj.org/toc/2077-0383Biogenic amines, such as adrenaline, noradrenaline, histamine, dopamine, and serotonin are important neurotransmitters that also regulate cell viability. Their detection and analysis are helpful in the diagnosis of many diseases, including cancer. The aim of this study was to determine the expression profile of the biogenic amine-related genes and proteins in endometrioid endometrial cancer compared to the control group. The material consisted of endometrial tissue samples and whole blood collected from 30 endometrioid endometrial cancer patients and 30 cancer-free patients. The gene expression was determined by the mRNA microarrays and validated by qRT-PCR. Protein levels were determined in the serum by the enzyme-linked immunosorbent assay (ELISA). Overexpression of histamine H1–H3 receptors and early growth response 1 and silencing of calmodulin, the histamine H4 receptor, and the dopamine D5 receptor have been reported in endometrioid endometrial cancer. The obtained results indicate disturbances in the signaling activated by histamine and dopamine receptors, which could potentially contribute to the progression of endometrioid endometrial cancer.Michał CzerwińskiAnna Bednarska-CzerwińskaNikola ZmarzłyDariusz BorońMarcin OplawskiBeniamin Oskar GrabarekMDPI AGarticleendometrial cancerbiogenic amineshistamine receptorsdopamine receptorsexpression profileMedicineRENJournal of Clinical Medicine, Vol 10, Iss 4872, p 4872 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	endometrial cancer biogenic amines histamine receptors dopamine receptors expression profile Medicine R
spellingShingle	endometrial cancer biogenic amines histamine receptors dopamine receptors expression profile Medicine R Michał Czerwiński Anna Bednarska-Czerwińska Nikola Zmarzły Dariusz Boroń Marcin Oplawski Beniamin Oskar Grabarek Evaluation of the Differences in the Expression of Biogenic Amine-Related mRNAs and Proteins in Endometrioid Endometrial Cancer
description	Biogenic amines, such as adrenaline, noradrenaline, histamine, dopamine, and serotonin are important neurotransmitters that also regulate cell viability. Their detection and analysis are helpful in the diagnosis of many diseases, including cancer. The aim of this study was to determine the expression profile of the biogenic amine-related genes and proteins in endometrioid endometrial cancer compared to the control group. The material consisted of endometrial tissue samples and whole blood collected from 30 endometrioid endometrial cancer patients and 30 cancer-free patients. The gene expression was determined by the mRNA microarrays and validated by qRT-PCR. Protein levels were determined in the serum by the enzyme-linked immunosorbent assay (ELISA). Overexpression of histamine H1–H3 receptors and early growth response 1 and silencing of calmodulin, the histamine H4 receptor, and the dopamine D5 receptor have been reported in endometrioid endometrial cancer. The obtained results indicate disturbances in the signaling activated by histamine and dopamine receptors, which could potentially contribute to the progression of endometrioid endometrial cancer.
format	article
author	Michał Czerwiński Anna Bednarska-Czerwińska Nikola Zmarzły Dariusz Boroń Marcin Oplawski Beniamin Oskar Grabarek
author_facet	Michał Czerwiński Anna Bednarska-Czerwińska Nikola Zmarzły Dariusz Boroń Marcin Oplawski Beniamin Oskar Grabarek
author_sort	Michał Czerwiński
title	Evaluation of the Differences in the Expression of Biogenic Amine-Related mRNAs and Proteins in Endometrioid Endometrial Cancer
title_short	Evaluation of the Differences in the Expression of Biogenic Amine-Related mRNAs and Proteins in Endometrioid Endometrial Cancer
title_full	Evaluation of the Differences in the Expression of Biogenic Amine-Related mRNAs and Proteins in Endometrioid Endometrial Cancer
title_fullStr	Evaluation of the Differences in the Expression of Biogenic Amine-Related mRNAs and Proteins in Endometrioid Endometrial Cancer
title_full_unstemmed	Evaluation of the Differences in the Expression of Biogenic Amine-Related mRNAs and Proteins in Endometrioid Endometrial Cancer
title_sort	evaluation of the differences in the expression of biogenic amine-related mrnas and proteins in endometrioid endometrial cancer
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/26fab4487c9644348bd0fc1dd5b9d71e
work_keys_str_mv	AT michałczerwinski evaluationofthedifferencesintheexpressionofbiogenicaminerelatedmrnasandproteinsinendometrioidendometrialcancer AT annabednarskaczerwinska evaluationofthedifferencesintheexpressionofbiogenicaminerelatedmrnasandproteinsinendometrioidendometrialcancer AT nikolazmarzły evaluationofthedifferencesintheexpressionofbiogenicaminerelatedmrnasandproteinsinendometrioidendometrialcancer AT dariuszboron evaluationofthedifferencesintheexpressionofbiogenicaminerelatedmrnasandproteinsinendometrioidendometrialcancer AT marcinoplawski evaluationofthedifferencesintheexpressionofbiogenicaminerelatedmrnasandproteinsinendometrioidendometrialcancer AT beniaminoskargrabarek evaluationofthedifferencesintheexpressionofbiogenicaminerelatedmrnasandproteinsinendometrioidendometrialcancer
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Evaluation of the Differences in the Expression of Biogenic Amine-Related mRNAs and Proteins in Endometrioid Endometrial Cancer

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