Cytotoxic and anti-excitotoxic effects of selected plant and algal extracts using COMET and cell viability assays

Abstract Excess glutamate in the central nervous system may be a major cause of neurodegenerative diseases with gradual loss and dysfunction of neurons. Primary or secondary metabolites from medicinal plants and algae show potential for treatment of glutamate-induced excitotoxicity. Three plant extr...

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Autores principales:	Abeer Aldbass, Musarat Amina, Nawal M. Al Musayeib, Ramesa Shafi Bhat, Sara Al-Rashed, Najat Marraiki, Rania Fahmy, Afaf El-Ansary
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/26fd58195cdb4967811ca9fcc3a3db29
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spelling	oai:doaj.org-article:26fd58195cdb4967811ca9fcc3a3db292021-12-02T16:45:10ZCytotoxic and anti-excitotoxic effects of selected plant and algal extracts using COMET and cell viability assays10.1038/s41598-021-88089-82045-2322https://doaj.org/article/26fd58195cdb4967811ca9fcc3a3db292021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88089-8https://doaj.org/toc/2045-2322Abstract Excess glutamate in the central nervous system may be a major cause of neurodegenerative diseases with gradual loss and dysfunction of neurons. Primary or secondary metabolites from medicinal plants and algae show potential for treatment of glutamate-induced excitotoxicity. Three plant extracts were evaluated for impact on glutamate excitotoxicity-induced in primary cultures of retinal ganglion cells (RGC). These cells were treated separately in seven groups: control; Plicosepalus. curviflorus treated; Saussurea lappa treated; Cladophora glomerate treated. Cells were treated independently with 5, 10, 50, or 100 µg/ml of extracts of plant or alga material, respectively, for 2 h. Glutamate-treated cells (48 h with 5, 10, 50, or 100 µM glutamate); and P. curviflorus/glutamate; S. lappa/glutamate; C. glomerata/glutamate [pretreatment with extract for 2 h (50 and 100 µg/ml) before glutamate treatment with 100 µM for 48 h]. Comet and MTT assays were used to assess cell damage and cell viability. The number of viable cells fell significantly after glutamate exposure. Exposure to plant extracts caused no notable effect of viability. All tested plants extracts showed a protective effect against glutamate excitotoxicity-induced RGC death. Use of these extracts for neurological conditions related to excitotoxicity and oxidative stress might prove beneficial.Abeer AldbassMusarat AminaNawal M. Al MusayeibRamesa Shafi BhatSara Al-RashedNajat MarraikiRania FahmyAfaf El-AnsaryNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Abeer Aldbass Musarat Amina Nawal M. Al Musayeib Ramesa Shafi Bhat Sara Al-Rashed Najat Marraiki Rania Fahmy Afaf El-Ansary Cytotoxic and anti-excitotoxic effects of selected plant and algal extracts using COMET and cell viability assays
description	Abstract Excess glutamate in the central nervous system may be a major cause of neurodegenerative diseases with gradual loss and dysfunction of neurons. Primary or secondary metabolites from medicinal plants and algae show potential for treatment of glutamate-induced excitotoxicity. Three plant extracts were evaluated for impact on glutamate excitotoxicity-induced in primary cultures of retinal ganglion cells (RGC). These cells were treated separately in seven groups: control; Plicosepalus. curviflorus treated; Saussurea lappa treated; Cladophora glomerate treated. Cells were treated independently with 5, 10, 50, or 100 µg/ml of extracts of plant or alga material, respectively, for 2 h. Glutamate-treated cells (48 h with 5, 10, 50, or 100 µM glutamate); and P. curviflorus/glutamate; S. lappa/glutamate; C. glomerata/glutamate [pretreatment with extract for 2 h (50 and 100 µg/ml) before glutamate treatment with 100 µM for 48 h]. Comet and MTT assays were used to assess cell damage and cell viability. The number of viable cells fell significantly after glutamate exposure. Exposure to plant extracts caused no notable effect of viability. All tested plants extracts showed a protective effect against glutamate excitotoxicity-induced RGC death. Use of these extracts for neurological conditions related to excitotoxicity and oxidative stress might prove beneficial.
format	article
author	Abeer Aldbass Musarat Amina Nawal M. Al Musayeib Ramesa Shafi Bhat Sara Al-Rashed Najat Marraiki Rania Fahmy Afaf El-Ansary
author_facet	Abeer Aldbass Musarat Amina Nawal M. Al Musayeib Ramesa Shafi Bhat Sara Al-Rashed Najat Marraiki Rania Fahmy Afaf El-Ansary
author_sort	Abeer Aldbass
title	Cytotoxic and anti-excitotoxic effects of selected plant and algal extracts using COMET and cell viability assays
title_short	Cytotoxic and anti-excitotoxic effects of selected plant and algal extracts using COMET and cell viability assays
title_full	Cytotoxic and anti-excitotoxic effects of selected plant and algal extracts using COMET and cell viability assays
title_fullStr	Cytotoxic and anti-excitotoxic effects of selected plant and algal extracts using COMET and cell viability assays
title_full_unstemmed	Cytotoxic and anti-excitotoxic effects of selected plant and algal extracts using COMET and cell viability assays
title_sort	cytotoxic and anti-excitotoxic effects of selected plant and algal extracts using comet and cell viability assays
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/26fd58195cdb4967811ca9fcc3a3db29
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Cytotoxic and anti-excitotoxic effects of selected plant and algal extracts using COMET and cell viability assays

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