Guidelines and Considerations for Metabolic Tolerance Tests in Mice

Raquel Benedé-Ubieto,1,* Olga Estévez-Vázquez,1,* Pierluigi Ramadori,2 Francisco Javier Cubero,3,4 Yulia A Nevzorova1,4,5 1Department of Physiology, Genetics and Microbiology, Faculty of Biology, Complutense University, Madrid, Spain; 2Division of Chronic Inflammatio...

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Autores principales:	Benedé-Ubieto R, Estévez-Vázquez O, Ramadori P, Cubero FJ, Nevzorova YA
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	metabolic tolerance test t2dm obesity metabolic syndrome insulin resistance glucose intolerance. Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/2700436fe7f04a97bb83321911aafcdd
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spelling	oai:doaj.org-article:2700436fe7f04a97bb83321911aafcdd2021-12-02T12:35:00ZGuidelines and Considerations for Metabolic Tolerance Tests in Mice1178-7007https://doaj.org/article/2700436fe7f04a97bb83321911aafcdd2020-02-01T00:00:00Zhttps://www.dovepress.com/guidelines-and-considerations-for-metabolic-tolerance-tests-in-mice-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Raquel Benedé-Ubieto,1,* Olga Estévez-Vázquez,1,* Pierluigi Ramadori,2 Francisco Javier Cubero,3,4 Yulia A Nevzorova1,4,5 1Department of Physiology, Genetics and Microbiology, Faculty of Biology, Complutense University, Madrid, Spain; 2Division of Chronic Inflammation and Cancer, German Cancer Research Center Heidelberg (DKFZ), Heidelberg, Germany; 3Department of Immunology, Ophthalmology & ENT, Complutense University School of Medicine, Madrid, Spain; 4 12 de Octubre Health Research Institute (imas12), Madrid, Spain; 5Department of Internal Medicine III, University Hospital RWTH Aachen, Aachen, Germany*These authors contributed equally to this workCorrespondence: Yulia A NevzorovaDepartment of Physiology, Genetics and Microbiology, Faculty of Biology, Complutense University, c/José A. Novais, 2, Madrid 28040, SpainTel +49-(0)241-80-80662Fax +49-(0)241-80-82455Email ynevzorova@ukaachen.deAbstract: The epidemic of the century, Diabetes Mellitus (DM) is continuously rising. Intensive research is urgently needed whereby experimental models represent an essential tool to optimise the diagnostic strategy and to improve therapy. In this review, we describe the central principles of the metabolic tests available in order to study glucose and insulin homeostasis in mice, focusing on the most widely used – the glucose and insulin tolerance tests. We provide detailed experimental procedures as well as the practical implementation of these methods and discuss the main factors that should be taken into account when using this methodology.Keywords: metabolic tolerance test, T2DM, obesity, metabolic syndrome, insulin resistance, glucose intoleranceBenedé-Ubieto REstévez-Vázquez ORamadori PCubero FJNevzorova YADove Medical Pressarticlemetabolic tolerance testt2dmobesitymetabolic syndromeinsulin resistanceglucose intolerance.Specialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 13, Pp 439-450 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	metabolic tolerance test t2dm obesity metabolic syndrome insulin resistance glucose intolerance. Specialties of internal medicine RC581-951
spellingShingle	metabolic tolerance test t2dm obesity metabolic syndrome insulin resistance glucose intolerance. Specialties of internal medicine RC581-951 Benedé-Ubieto R Estévez-Vázquez O Ramadori P Cubero FJ Nevzorova YA Guidelines and Considerations for Metabolic Tolerance Tests in Mice
description	Raquel Benedé-Ubieto,1,* Olga Estévez-Vázquez,1,* Pierluigi Ramadori,2 Francisco Javier Cubero,3,4 Yulia A Nevzorova1,4,5 1Department of Physiology, Genetics and Microbiology, Faculty of Biology, Complutense University, Madrid, Spain; 2Division of Chronic Inflammation and Cancer, German Cancer Research Center Heidelberg (DKFZ), Heidelberg, Germany; 3Department of Immunology, Ophthalmology & ENT, Complutense University School of Medicine, Madrid, Spain; 4 12 de Octubre Health Research Institute (imas12), Madrid, Spain; 5Department of Internal Medicine III, University Hospital RWTH Aachen, Aachen, Germany*These authors contributed equally to this workCorrespondence: Yulia A NevzorovaDepartment of Physiology, Genetics and Microbiology, Faculty of Biology, Complutense University, c/José A. Novais, 2, Madrid 28040, SpainTel +49-(0)241-80-80662Fax +49-(0)241-80-82455Email ynevzorova@ukaachen.deAbstract: The epidemic of the century, Diabetes Mellitus (DM) is continuously rising. Intensive research is urgently needed whereby experimental models represent an essential tool to optimise the diagnostic strategy and to improve therapy. In this review, we describe the central principles of the metabolic tests available in order to study glucose and insulin homeostasis in mice, focusing on the most widely used – the glucose and insulin tolerance tests. We provide detailed experimental procedures as well as the practical implementation of these methods and discuss the main factors that should be taken into account when using this methodology.Keywords: metabolic tolerance test, T2DM, obesity, metabolic syndrome, insulin resistance, glucose intolerance
format	article
author	Benedé-Ubieto R Estévez-Vázquez O Ramadori P Cubero FJ Nevzorova YA
author_facet	Benedé-Ubieto R Estévez-Vázquez O Ramadori P Cubero FJ Nevzorova YA
author_sort	Benedé-Ubieto R
title	Guidelines and Considerations for Metabolic Tolerance Tests in Mice
title_short	Guidelines and Considerations for Metabolic Tolerance Tests in Mice
title_full	Guidelines and Considerations for Metabolic Tolerance Tests in Mice
title_fullStr	Guidelines and Considerations for Metabolic Tolerance Tests in Mice
title_full_unstemmed	Guidelines and Considerations for Metabolic Tolerance Tests in Mice
title_sort	guidelines and considerations for metabolic tolerance tests in mice
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/2700436fe7f04a97bb83321911aafcdd
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Guidelines and Considerations for Metabolic Tolerance Tests in Mice

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