Investigation of the effects of carbon-based nanomaterials on A53T alpha-synuclein aggregation using a whole-cell recombinant biosensor
Soheila Mohammadi,1 Maryam Nikkhah,1 Saman Hosseinkhani2 1Department of Nanobiotechnology, 2Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran Abstract: The aggregation of alpha-synuclein (αS), natively unstructured presynaptic protein, i...
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oai:doaj.org-article:2707528d23b64880a92716fa6051ac782021-12-02T05:10:25ZInvestigation of the effects of carbon-based nanomaterials on A53T alpha-synuclein aggregation using a whole-cell recombinant biosensor1178-2013https://doaj.org/article/2707528d23b64880a92716fa6051ac782017-12-01T00:00:00Zhttps://www.dovepress.com/investigation-of-the-effects-of-carbon-based-nanomaterials-on-a53t-alp-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Soheila Mohammadi,1 Maryam Nikkhah,1 Saman Hosseinkhani2 1Department of Nanobiotechnology, 2Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran Abstract: The aggregation of alpha-synuclein (αS), natively unstructured presynaptic protein, is a crucial factor leading to the pathogenesis of Parkinson’s disease (PD) and other related disorders. Recent studies have shown prefibrillar and oligomeric intermediates of αS as toxic to the cells. Herein, split-luciferase complementation assay is used to design a “signal-on” biosensor to monitor oligomerization of A53T αS inside the cells. Then, the effect of carbon-based nanomaterials, such as graphene quantum dots (GQDs) and graphene oxide quantum dots (GOQDs), on A53T αS oligomerization in vitro and in living cells is investigated. In this work, for the first time, it was found that GQDs at a concentration of 0.5 µg/mL can promote A53T αS aggregation by shortening the nucleation process, which is the key rate-determining step of fibrillation, thereby making a signal-on biosensor. While these nanomaterials may cross the blood–brain barrier because of their small sizes, the interaction between αS and GQDs may contribute to PD etiology. Keywords: αS, aggregation, split luciferase, luminescent biosensor, GQDs Mohammadi SNikkhah MHosseinkhani SDove Medical Pressarticleα-synuclein (αS)split luciferaseluminescent biosensorgraphene quantum dots (GQDs)Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 8831-8840 (2017) |
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α-synuclein (αS) split luciferase luminescent biosensor graphene quantum dots (GQDs) Medicine (General) R5-920 |
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α-synuclein (αS) split luciferase luminescent biosensor graphene quantum dots (GQDs) Medicine (General) R5-920 Mohammadi S Nikkhah M Hosseinkhani S Investigation of the effects of carbon-based nanomaterials on A53T alpha-synuclein aggregation using a whole-cell recombinant biosensor |
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Soheila Mohammadi,1 Maryam Nikkhah,1 Saman Hosseinkhani2 1Department of Nanobiotechnology, 2Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran Abstract: The aggregation of alpha-synuclein (αS), natively unstructured presynaptic protein, is a crucial factor leading to the pathogenesis of Parkinson’s disease (PD) and other related disorders. Recent studies have shown prefibrillar and oligomeric intermediates of αS as toxic to the cells. Herein, split-luciferase complementation assay is used to design a “signal-on” biosensor to monitor oligomerization of A53T αS inside the cells. Then, the effect of carbon-based nanomaterials, such as graphene quantum dots (GQDs) and graphene oxide quantum dots (GOQDs), on A53T αS oligomerization in vitro and in living cells is investigated. In this work, for the first time, it was found that GQDs at a concentration of 0.5 µg/mL can promote A53T αS aggregation by shortening the nucleation process, which is the key rate-determining step of fibrillation, thereby making a signal-on biosensor. While these nanomaterials may cross the blood–brain barrier because of their small sizes, the interaction between αS and GQDs may contribute to PD etiology. Keywords: αS, aggregation, split luciferase, luminescent biosensor, GQDs |
format |
article |
author |
Mohammadi S Nikkhah M Hosseinkhani S |
author_facet |
Mohammadi S Nikkhah M Hosseinkhani S |
author_sort |
Mohammadi S |
title |
Investigation of the effects of carbon-based nanomaterials on A53T alpha-synuclein aggregation using a whole-cell recombinant biosensor |
title_short |
Investigation of the effects of carbon-based nanomaterials on A53T alpha-synuclein aggregation using a whole-cell recombinant biosensor |
title_full |
Investigation of the effects of carbon-based nanomaterials on A53T alpha-synuclein aggregation using a whole-cell recombinant biosensor |
title_fullStr |
Investigation of the effects of carbon-based nanomaterials on A53T alpha-synuclein aggregation using a whole-cell recombinant biosensor |
title_full_unstemmed |
Investigation of the effects of carbon-based nanomaterials on A53T alpha-synuclein aggregation using a whole-cell recombinant biosensor |
title_sort |
investigation of the effects of carbon-based nanomaterials on a53t alpha-synuclein aggregation using a whole-cell recombinant biosensor |
publisher |
Dove Medical Press |
publishDate |
2017 |
url |
https://doaj.org/article/2707528d23b64880a92716fa6051ac78 |
work_keys_str_mv |
AT mohammadis investigationoftheeffectsofcarbonbasednanomaterialsona53talphasynucleinaggregationusingawholecellrecombinantbiosensor AT nikkhahm investigationoftheeffectsofcarbonbasednanomaterialsona53talphasynucleinaggregationusingawholecellrecombinantbiosensor AT hosseinkhanis investigationoftheeffectsofcarbonbasednanomaterialsona53talphasynucleinaggregationusingawholecellrecombinantbiosensor |
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1718400520749580288 |