Glucose inhibits haemostasis and accelerates diet-induced hyperlipidaemia in zebrafish larvae

Abstract Hyperglycaemia damages the microvasculature in part through the reduced recruitment of immune cells and interference with platelet signalling, leading to poor wound healing and accelerated lipid deposition in mammals. We investigated the utility of zebrafish larvae to model the effect of ex...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Simone Morris, Pradeep Manuneedhi Cholan, Warwick J. Britton, Stefan H. Oehlers
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2707e23cd20d44468938a193ff98e615
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2707e23cd20d44468938a193ff98e615
record_format	dspace
spelling	oai:doaj.org-article:2707e23cd20d44468938a193ff98e6152021-12-02T15:15:13ZGlucose inhibits haemostasis and accelerates diet-induced hyperlipidaemia in zebrafish larvae10.1038/s41598-021-98566-92045-2322https://doaj.org/article/2707e23cd20d44468938a193ff98e6152021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98566-9https://doaj.org/toc/2045-2322Abstract Hyperglycaemia damages the microvasculature in part through the reduced recruitment of immune cells and interference with platelet signalling, leading to poor wound healing and accelerated lipid deposition in mammals. We investigated the utility of zebrafish larvae to model the effect of exogenous glucose on neutrophil and macrophage recruitment to a tail wound, wound-induced haemostasis, and chicken egg yolk feed challenge-induced hyperlipidaemia by supplementing larvae with exogenous glucose by immersion or injection. Neither method of glucose supplementation affected the recruitment of neutrophils and macrophages following tail transection. Glucose injection reduced thrombocyte retention and fibrin plug formation while only thrombocyte retention was reduced by glucose immersion following tail transection. We observed accelerated lipid accumulation in glucose-injected larvae challenged with high fat chicken egg yolk feeding. Our study identifies conserved and divergent effects of high glucose on inflammation, haemostasis, and hyperlipidaemia in zebrafish larvae compared to mammals.Simone MorrisPradeep Manuneedhi CholanWarwick J. BrittonStefan H. OehlersNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Simone Morris Pradeep Manuneedhi Cholan Warwick J. Britton Stefan H. Oehlers Glucose inhibits haemostasis and accelerates diet-induced hyperlipidaemia in zebrafish larvae
description	Abstract Hyperglycaemia damages the microvasculature in part through the reduced recruitment of immune cells and interference with platelet signalling, leading to poor wound healing and accelerated lipid deposition in mammals. We investigated the utility of zebrafish larvae to model the effect of exogenous glucose on neutrophil and macrophage recruitment to a tail wound, wound-induced haemostasis, and chicken egg yolk feed challenge-induced hyperlipidaemia by supplementing larvae with exogenous glucose by immersion or injection. Neither method of glucose supplementation affected the recruitment of neutrophils and macrophages following tail transection. Glucose injection reduced thrombocyte retention and fibrin plug formation while only thrombocyte retention was reduced by glucose immersion following tail transection. We observed accelerated lipid accumulation in glucose-injected larvae challenged with high fat chicken egg yolk feeding. Our study identifies conserved and divergent effects of high glucose on inflammation, haemostasis, and hyperlipidaemia in zebrafish larvae compared to mammals.
format	article
author	Simone Morris Pradeep Manuneedhi Cholan Warwick J. Britton Stefan H. Oehlers
author_facet	Simone Morris Pradeep Manuneedhi Cholan Warwick J. Britton Stefan H. Oehlers
author_sort	Simone Morris
title	Glucose inhibits haemostasis and accelerates diet-induced hyperlipidaemia in zebrafish larvae
title_short	Glucose inhibits haemostasis and accelerates diet-induced hyperlipidaemia in zebrafish larvae
title_full	Glucose inhibits haemostasis and accelerates diet-induced hyperlipidaemia in zebrafish larvae
title_fullStr	Glucose inhibits haemostasis and accelerates diet-induced hyperlipidaemia in zebrafish larvae
title_full_unstemmed	Glucose inhibits haemostasis and accelerates diet-induced hyperlipidaemia in zebrafish larvae
title_sort	glucose inhibits haemostasis and accelerates diet-induced hyperlipidaemia in zebrafish larvae
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2707e23cd20d44468938a193ff98e615
work_keys_str_mv	AT simonemorris glucoseinhibitshaemostasisandacceleratesdietinducedhyperlipidaemiainzebrafishlarvae AT pradeepmanuneedhicholan glucoseinhibitshaemostasisandacceleratesdietinducedhyperlipidaemiainzebrafishlarvae AT warwickjbritton glucoseinhibitshaemostasisandacceleratesdietinducedhyperlipidaemiainzebrafishlarvae AT stefanhoehlers glucoseinhibitshaemostasisandacceleratesdietinducedhyperlipidaemiainzebrafishlarvae
_version_	1718387557375410176

Glucose inhibits haemostasis and accelerates diet-induced hyperlipidaemia in zebrafish larvae

Ejemplares similares